基于GQDs的荧光猝灭检测抗坏血酸的新方法(4),医学检验论文

基于GQDs的荧光猝灭检测抗坏血酸的新方法(4),医学检验论文4结论。建立了一种基于GQDs荧光猝灭检测AA的新方式方法，该方式方法操作简便，灵敏度高，检出限低至1.010-6mol/L,特异性强，一些糖类和氨基酸的存在均不干扰AA的测定，甚至是与AA有类似电化学性质的DA、UA的存在对AA的检测也没有影响。本实验用到的所有原料都价廉易得且不需要任何修饰经过，GQDs相对于其他量子点的合成更简单而且具有低毒性，有望应用于生物体内生物活性物质的检测。参考文献：[1]PONOMARENKOLA,SCHEDINF,KATSNELSONMI,etal..Chaoticdiracbilliardingraphenequantumdots[J].Science,2008,320〔5874〕:356-358.[2]PANDY,ZHANGJC,LIZ,etal..Hydrothermalrouteforcuttinggraphenesheetsintoblue-luminescentgraphenequantumdots[J].Adv.Mater.,2018,22〔6〕:734-738.[3]CHENGHH,ZHAOY,FANYQ,etal..Graphene-quantum-dotassemblednanotubes:anewplatformforefficientRa-manenhancement[J].ACSNano,2020,6〔3〕:2237-2244.[4]DONGYQ,CHENCQ,ZHENGXT,etal..One-stepandhighyieldsimultaneouspreparationofsingle-andmulti-layergraphenequantumdotsfromCX-72carbonblack[J].J.Mater.Chem.,2020,22〔18〕:8764-8766.[5]SHENJH,ZHUYH,YANGXL,etal..Graphenequantumdots:emergentnanolightsforbioimaging,sensors,cataly-sisandphotovoltaicdevices[J].Chem.Commun.,2020,48〔31〕:3686-3699.[6]LUJ,YEOPSE,GANCK,etal..TransformingC60moleculesintographenequantumdots[J].Nat.Nanotechnol.,2018,6〔4〕:247-252.[7]LIURL,WUDQ,FENGXL,etal..Bottom-upfabricationofphotoluminescentgraphenequantumdotswithuniformmorphology[J].J.Am.Chem.Soc.,2018,133〔39〕:15221-15223.[8]NIUZQ,CHENJ,HNGHH,etal..Aleaveningstrategytopreparereducedgrapheneoxidefoams[J].Adv.Mater.,2020,24〔30〕:4144-4150.[9]DONGYQ,LIGL,ZHOUNN,etal..Graphenequantumdotasagreenandfacilesensorforfreechlorineindrinkingwater[J].Anal.Chem.,2020,84〔19〕:8378-8382.[10]BAIJM,ZHANGL,LIANGRP,etal..Graphenequantumdotscombinedwitheuropiumionsasphotoluminescentprobesforphosphatesensing[J].Chem.Eur.J.,2020,19〔12〕:3822-3826.[11]LIUJJ,ZHANGXL,CONGZX,etal..Glutathione-functionalizedgraphenequantumdotsasselectivefluorescentprobesforphosphate-containingmetabolites[J].Nanoscale,2020,5〔5〕:1810-1815.[12]WANGD,WANGL,DONGXY,etal..ChemicallytailoringgrapheneoxidesintofluorescentnanosheetsforFe3+iondetection[J].Carbon,2020,50〔6〕:2147-2154.[13]ZHANGY,WUCY,ZHOUXJ,etal..Graphenequantumdots/goldelectrodeanditsapplicationinlivingcellH2O2detection[J].Nanoscale,2020,5〔5〕:1816-1819.[14]LIYH,ZHANGL,HUANGJ,etal.Fluorescentgraphenequantumdotswithaboronicacidappendedbipyridiniumsalttosensemonosaccharidesinaqueoussolution[J].Chem.Commun.,2020,49〔45〕:5180-5182.[15]RAZMIH,MOHAMMAD-REZAEIR.Graphenequantumdotsasanewsubstrateforimmobilizationanddirectelectro-chemistryofglucoseoxidase:applicationtosensitiveglucosedetermination[J].Biosens.Bioelectron.,2020,41:498-504.[16]ZHAOHM,CHANGYY,LIUM,etal..Auniversalimmunosensingstrategybasedonregulationoftheinteractionbe-tweengrapheneandgraphenequantumdots[J].Chem.Commun.,2020,49〔3〕:234-236.[17]RANX,SUNHJ,PUF,etal..Agnanoparticle-decoratedgraphenequantumdotsforlabel-free,rapidandsensitivede-tectionofAg+andbiothiols[J].Chem.Commun.,2020,49〔11〕:1079-1081.[18]ZHOUL,LINYH,HUANGZZ,etal..Carbonnanodotsasfluorescenceprobesforrapid,sensitive,andlabel-freede-tectionofHg2+andbiothiolsincomplexmatrices[J].Chem.Commun.,2020,48〔8〕:1147-1149.[19]ZHAOJ,CHENGF,ZHUL,etal..Graphenequantumdots-basedplatformforthefabricationofelectrochemicalbiosen-sors[J].Electrochem.Commun.,2018,13〔1〕:31-33.[20]LIX,ZHUSJ,XUB,etal.Self-assembledgraphenequantumdotsinducedbycytochromeC:anovelbiosensorfortryp-sinwithremarkablefluorescenceenhancement[J].Nanoscale,2020,5〔17〕:7776-7779.[21]JINGYJ,ZHUYH,YANGXL,etal..Ultrasound-triggeredsmartdrugreleasefrommultifunctionalcore-shellcapsulesone-stepfabricatedbycoaxialelectrospraymethod[J].Langmuir,2018,27〔3〕:1175-1180.[22]PENGJ,GAOW,GUPTABK,etal..Graphenequantumdotsderivedfromcarbonfibers[J].NanoLett.,2020,12〔2〕:844-849.[23]ZHUSJ,ZHANGJH,QIAOCY,etal..Stronglygreen-photoluminescentgraphenequantumdotsforbioimagingappli-cations[J].Chem.Commun.,2018,47〔24〕:6858-6860.[24]ZHANGM,BAILL,SHANGWH,etal..Facilesynthesisofwater-soluble,highlyfluorescentgraphenequantumdotsasarobustbiologicallabelforstemcells[J].J.Mater.Chem.,2020,22〔15〕:7461-7467.[25]XIAOLF,CHENJ,CHACS.Eliminationoftheinterferenceofascorbicacidintheamperometricdetectionofbiomole-culesinbodyfluidsamplesandthesimpledetectionofuricacidinhumanserumandurinebyusingthepowdermicroelec-trodetechnique[J].J.Electroanal.Chem.,2000,495〔1〕:27-35.[26]SOODSP,SARTORILE,WITTMERDP,etal..High-pressureliquidchromatographicdeterminationofascorbicacidinselectedfoodsandmultivitaminproducts[J].Anal.Chem.,1976,48〔6〕:796-798.[27]WANGZH,LIUJ,LIANGQL,etal..Carbonnanotube-modifiedelectrodesforthesimultaneousdeterminationofdopa-mineandascorbicacid[J].Analyst,2002,127〔5〕:653-658.[28]CHENH,LIRB,LINL,etal..DeterminationofL-ascorbicacidinhumanserumbychemiluminescencebasedonhy-drogenperoxide-sodiumhydrogencarbonate-CdSe/CdSquantumdotssystem[J].Talanta,2018,81〔4-5〕:1688-1696.[29]SHAKYAR,NAVARREDA.Rapidscreeningofascorbicacid,glycoalkaloids,andphenolicsinpotatousinghigh-per-formanceliquidchromatography[J].J.Agric.FoodChem.,2006,54〔15〕:5253-5260.[30]BOSSIA,PILETSKYSA,PILETSKAEV,etal..Anassayforascorbicacidbasedonpolyaniline-coatedmicroplates[J].Anal.Chem.,2000,72〔18〕:4296-4300.[31]CASTELLETT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