穴位肥大细胞及其颗粒物在针刺镇痛中的作用课件

Outline穴位肥大细胞在针刺镇痛效应中的作用穴位胶原纤维在针刺镇痛效应中的作用穴位注射组胺的镇痛效应手针和电针穴位信号的启动机制MastcellsinacupointsandsidewardComparisonofNumberofMCsinZhushanliandnon-acupointsofRatGroupNameMCsNumberSkinMuscleAcupoint（n＝6）34.2±2.512.4±1.4Non-acupoint（n＝6）22.1±1.7*8.5±1.0*THEJOURNALOFSCIENCE&HEALING,2008，4（3），170-1771.Therelationshipbetween

mastcellfunctionandacupunctureeffectAnimalmodel：AA(acutearthritis)RatCompleteFreunds’adjuvantAcupointselected：Zushanli(ST36)

DST

36C1.Therelationshipbetween

mastcellfunctionandacupunctureeffectTreateffect：Easepain，Painthreshold1.Therelationshipbetween

mastcellfunctionandacupunctureeffectThedegranulationofmastcellsSDRatsTailFlickModelAnatomyPositionofZusanliAcupunctureInjectiondrugsforpreventdegranulationofMCABDEST

36CPainthresholdofratwhenshieldmastcelldegranulation1.Therelationshipbetween

mastcellfunctionandacupunctureeffectIDGroupName组别Average±SEM(%)AControl正常对照组–1.9±0.1B1AcupunctureatST36穴位针刺组11.6±0.6*B2AcupunctureatNearby-ST36旁开针刺组8.9±0.3*△△C1InjectionofDSCGatST36色甘酸钠注射组1.7±0.1*△C2InjectionofNaClatST36生理盐水注射组1.4±0.1*△D1AcupunctureafterDSCGatST36色甘酸钠注射＋针刺组6.2±0.4*△△D2AcupunctureafterNaClatST36生理盐水注射＋针刺组8.9±0.4*▲D3AcupunctureoppositesideafterDSCG色甘酸钠注射＋对侧针刺组9.3±0.1*▲▲各组别大鼠痛阈变化率峰值比较（20分钟时）(n=6)Note:*P<0.05vs.GroupA;△P<0.01,

△△P<0.05vs.GroupB1;▲P<0.01,

▲▲P<0.05vs.GroupD11.Therelationshipbetween

mastcellfunctionandacupunctureeffectGroupName组别MCDegranulationRatio

(Average±SEM)(％)SkinMuscleControl正常对照组29.2±1.226.3±1.8Acupuncture穴位针刺组48.9±3.9*32.2±3.1**AcupunctureafterDSCG色甘酸钠注射＋针刺组33.7±2.8△28.5±2.3AcupunctureafterNaCl生理盐水注射＋针刺组50.4±2.5*29.7±2.3穴区组织肥大细胞脱颗粒率统计

(n=6)Note:*P<0.01,**P<0.05vs.ControlGroup;△P<0.01vs.AcupunctureGroup.ResultsfromExperimentStainingofMastCellsinAcupointonRatsMuscleSkinBeforeAcupunctureAfterAcupunctureDegranulationofcellsAfterSDCGStabilizedTHEJOURNALOFSCIENCE&HEALING,2008，4（3），170-177WhatisthekeyfactortodegranulationTheacupunctureeffectispositiverelationshiptothedegranulationofmastcellatacupoint.Whatiskeyfactorconducethedegranulationofmastcellduringacupuncturemanipulation?Ourfocusisonthecollagenatacupoint.2023/6/912ToobservethefunctionofcollagenfibersinMAanalgesia.7

Animalgroupssetting:normalgroup(N):Normalhealthyanimalcontrolgroup(M):AnimalwithAAinjectionofcollagenaseatZusanli(ST36)group(C)manualacupuncturegroupwithrotationmanipulation(R)orlift-thrustmanipulation(L),rotationgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CR),lift-thrustgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CL),lift-thrustgrouppretreatedwithcollagenasetodestroyDubi(ST35)collagenfibers(CDL)(R)(L)2023/6/913AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：较正常组痛阈：*P<0.01；较处理前痛阈：

P<0.05；较捻转组(R)痛阈变化率：▽P<0.05；较捻转组(L)痛阈变化率：▼P<0.05；较正常组或模型组:

×P<0.05；较捻转组或提插组:

△P<0.05。2023/6/914AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：较正常组痛阈：*P<0.01；较处理前痛阈：

P<0.05；较捻转组(R)痛阈变化率：▽P<0.05；较捻转组(L)痛阈变化率：▼P<0.05；较正常组或模型组:

×P<0.05；较捻转组或提插组:

△P<0.05。01与组胺穴位注射组比较，□P<0.Note:*P<0.01与手针针刺组比较（vs.通过H1受体进一步传递针刺信号01与手针针刺组比较（vs.较捻转组(R)痛阈变化率：▽P<0.PainThreshold(PT)组胺H1拮抗剂穴位注射组注：与正常组相比较：*P<0.01与生理盐水穴位注射组比较；MCDegranulationRatio(%)AfterSDCGStabilizedAfterMA(s)PainthresholdofratwhenshieldmastcelldegranulationInjectiondrugsforpreventdegranulationofMC2023/6/915AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：较正常组痛阈：*P<0.01；较处理前痛阈：

P<0.05；较捻转组(R)痛阈变化率：▽P<0.05；较捻转组(L)痛阈变化率：▼P<0.05；较正常组或模型组:

×P<0.05；较捻转组或提插组:

△P<0.05。collagenfibersofskinandmuscleMastcellsinskinGroupNormalGroupInjectControlcollagenfibersofskinandmuscleMastcellsinskinConnectiveTissueResearch,2008，50（2）:110-120Acupunc.whirlingAcupunc.liftandthrustcollagenfibersofskinandmuscleMastcellsinskincollagenfibersofskinandmuscleMastcellsinskinConnectiveTissueResearch,2008，50（2）:110-120

Mastcellsdegranulationbeforeandaftercollagendestroyed

CollagendestroyedCollagendestroyedConnectiveTissueResearch,2008，50（2）:110-120collagenfibersandmastcellsinnormalacupointcollagenfibersenlacementandmastcellsdegranulationwhilewhirlingcollagenfiberstightenedandmastcellsdegranulatedwhileliftingandthrustingcollagenfibersweredestroyedandmastcellshadnodegranulationConnectiveTissueResearch,2008，50（2）:110-120较捻转组或提插组:△P<0.注：较正常组痛阈：*P<0.较正常组或模型组:×P<0.75.degranulatedwhileliftingandthrustingAnimalmodel：AA(acutearthritis)RatDubi(ST35)collagenfibers(CDL)与模型组相比较：△P<0.穴位注射组胺的镇痛效应Therelationshipbetween

mastcellfunctionandacupunctureeffectPainThreshold(PT)Thedegranulationofmastcells较处理前痛阈：

P<0.WhatisthekeyfactortodegranulationConnectiveTissueResearch,2008，50（2）:110-1202023/6/920Themastcellsinacupointmaytranslatethemechanicalsignalintothebiologicalinformationofneuralsystem.Thecollagenandmastcellsdegranulationareveryimportanttothemanualacupunctureanalgesiaeffect.Isthisthesameaselectricacupuncture?2023/6/921组别n痛阈(s)痛阈变化率(%)肥大细胞脱颗粒率(%)正常组89.19±0.65-10.6模型组85.61±0.48*-14.1手针组87.52±0.65△38.575.4△电针组88.67±1.06△45.973.3△胶原酶+手针组85.39±0.57×3.210.4××胶原酶+电针组88.30±1.2347.619.0▲色甘酸钠+手针组86.00±0.48××5.825.1××色甘酸钠+电针组87.34±0.70▲27.529.5▲手针及电针治疗前后AA大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化注：与正常组相比较：*P<0.05；与模型组相比较：△P<0.05；与手针组相比较：××P<0.05；×P<0.01；与电针组相比较：▲P<0.05。3.穴位皮下注射组胺的镇痛疗效组别n痛阈(s)基础痛阈造模后痛阈治疗后痛阈空白对照组129.04±0.209.38±0.199.27±0.17模型组128.90±0.406.48±0.28﹡6.58±0.35△生理盐水对照组129.20±0.286.09±0.30﹡6.61±0.31△手针组129.52±0.186.58±0.17﹡8.77±0.26＊组胺注射组129.21±0.206.27±0.22﹡8.50±0.28＊☆各组痛阈测量比较3.穴位皮下注射组胺的镇痛疗效组别n痛阈(s)基础痛阈造模后痛阈治疗后痛阈空白对照组129.04±0.209.38±0.199.27±0.17模型组128.90±0.406.48±0.28﹡6.58±0.35△生理盐水对照组129.20±0.286.09±0.30﹡6.61±0.31△手针组129.52±0.186.58±0.17﹡8.77±0.26＊组胺注射组129.21±0.206.27±0.22﹡8.50±0.28＊☆各组痛阈测量比较针刺研究，2010，35（2）：99-1033.穴位皮下注射组胺的镇痛疗效组别n痛阈(s)基础痛阈造模后痛阈治疗后痛阈空白对照组129.04±0.209.38±0.199.27±0.17模型组128.90±0.406.48±0.28﹡6.58±0.35△生理盐水对照组129.20±0.286.09±0.30﹡6.61±0.31△手针组129.52±0.186.58±0.17﹡8.77±0.26＊组胺注射组129.21±0.206.27±0.22﹡8.50±0.28＊☆各组痛阈测量比较针刺研究，2010，35（2）：99-103lift-thrustgrouppretreatedwithcollagenasetodestroy针刺研究，2010，35（2）：99-103色甘酸钠预处理胺注射组Zusanli(ST36)collagenfibers(CR),01与空白组比较（vs.Zusanli(ST36)collagenfibers(CL),Acupuncture穴位皮下注射组胺的镇痛疗效各组别大鼠痛阈变化率峰值比较（20分钟时）(n=6)collagenfibersandmastcellsBeforeAcupuncture色甘酸钠预处理胺注射组较处理前痛阈：

P<0.ResultsfromExperiment穴位肥大细胞在针刺镇痛效应中的作用3.组胺H1受体在传递针刺信号中的作用

各组足三里穴位处脱颗粒比较组别N脱颗粒率空白组1233.59±0.72模型组1239.71±2.09﹡生理盐水穴位注射组1236.67±2.36手针针刺组1257.61±1.42＊组胺穴位注射组1257.03±2.95＊◆色甘酸钠预处理胺注射组1325.40±1.80▼色甘酸钠预处理针刺组1236.03±2.28△组胺H1拮抗剂预处理针刺组1251.54±2.32□组胺H1拮抗剂组胺注射组1237.13±1.90▼组胺H1拮抗剂穴位注射组2232.24±1.40﹡P<0.01与空白组比较（vs.control）；＊P<0.01与模型组比较（vs.model）；△P<0.01与手针针刺组比较（vs.control）；◆P<0.01与生理盐水穴位注射组比较；▼P<0.01与组胺穴位注射组比较，□P<0.01与组胺H1拮抗剂穴位注射组比较。针刺研究，2010，35（2）：99-1033.组胺H1受体在传递针刺信号中的作用

各组足三里穴位处脱颗粒比较组别N脱颗粒率空白组1233.59±0.72模型组1239.71±2.09﹡生理盐水穴位注射组1236.67±2.36手针针刺组1257.61±1.42＊组胺穴位注射组1257.03±2.95＊◆色甘酸钠预处理胺注射组1325.40±1.80▼色甘酸钠预处理针刺组1236.03±2.28△组胺H1拮抗剂预处理针刺组1251.54±2.32□组胺H1拮抗剂组胺注射组1237.13±1.90▼组胺H1拮抗剂穴位注射组2232.24±1.40﹡P<0.01与空白组比较（vs.control）；＊P<0.01与模型组比较（vs.model）；△P<0.01与手针针刺组比较（vs.control）；◆P<0.01与生理盐水穴位注射组比较；▼P<0.01与组胺穴位注射组比较，□P<0.01与组胺H1拮抗剂穴位注射组比较。针刺研究，2010，35（2）：99-103穴位处组胺及H1受体组胺参与到了针刺麻醉的穴位局部机制通过H1受体进一步传递针刺信号研究阐明了手针和电针穴位针刺有效信号的启动机制MAEAElectricA.ThankyouResultsfromExperimentStainingofMastCellsinAcupointonRatsMuscleSkinBeforeAcupunctureAfterAcupunctureDegranulationofcellsAfterSDCGStabilizedTHEJOURNALOFSCIENCE&HEALING,2008，4（3），170-177WhatisthekeyfactortodegranulationTheacupunctureeffectispositiverelationshiptothedegranulationofmastcellatacupoint.Whatiskeyfactorconducethedegranulationofmastcellduringacupuncturemanipulation?Ourfocusisonthecollagenatacupoint.2023/6/932ToobservethefunctionofcollagenfibersinMAanalgesia.7

Animalgroupssetting:normalgroup(N):Normalhealthyanimalcontrolgroup(M):AnimalwithAAinjectionofcollagenaseatZusanli(ST36)group(C)manualacupuncturegroupwithrotationmanipulation(R)orlift-thrustmanipulation(L),rotationgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CR),lift-thrustgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CL),lift-thrustgrouppretreatedwithcollagenasetodestroyDubi(ST35)collagenfibers(CDL)(R)(L)Mastcellsinskincollagenfibersofskinandmuscleorlift-thrustmanipulation(L),InjectiondrugsforpreventdegranulationofMCNon-acupointWhatisthekeyfactortodegranulationTHEJOURNALOFSCIENCE&HEALING,2008，4（3），170-177Thedegranulationofmastcells10.CompleteFreunds’adjuvantAnalgesiaeffectsobservedindifferentgroups73.ChangeRate(%)较正常组或模型组:×P<0.注：较正常组痛阈：*P<0.2023/6/933AnalgesiaeffectsobservedindifferentgroupsGroupnPainThreshold(PT)4dBeforeMA(s)AfterMA(s)ChangeRate(%)MCDegranulationRatio

(%)N89.19±0.65--10.6±2.6M85.61±0.48*--14.1±5.9C85.90±0.24*5.59±0.52-5.111.9±4.7△R85.44±0.37*7.52±0.65

38.575.7±4.4×L86.13±0.82*8.50±1.56

39.176.9±5.9×CR85.27±0.62*5.39±0.573.3▽10.4±2.4△CL86.05±0.43*6.26±0.793.8▼12.4±2.3△注：较正常组痛阈：*P<0.01；较处理前痛阈：

P<0.05；较捻转组(R)痛阈变化率：▽P<0.05；较捻转组(L)痛阈变化率：▼P<0.05；较正常组或模型组:

×P<0.05；较捻转组或提插组:

△P<0.05。Acupunc.whirlingAcupunc.liftandthrustcollagenfibersofskinandmuscleMastcellsinskincollagenfibersofskinandmuscleMastcellsinskinConnectiveTissueResearch,2008，50（2）:110-120InjectiondrugsforpreventdegranulationofMCResult