荧光探针与分子传感器教学课件

郭祥群博士,博士生导师全国高校化学教育研究中心第四届学术委员会副主任教育部高等学校本科教学工作水平评估专家,中国化学会理事◆先后获得国家级奖2项,省部级奖4项,发表科研与教学研究论文100余篇。◆研究兴趣:荧光光谱分析的原理与技术,在研项目涉及生物活性自由基的荧光表征,海洋污染生物监测技术研究,食品安全等◆联系方式:X三m20592-2181637(L),2187288(O,2182355(H20050705UORESCENCESPECTROSCOPFluorescentProbes◆Introduction◆Fluorescentsensor荧光传感◆FluorescentTracer荧光示踪◆FRET共振能量转移荧光◆Application1.Radicalprobes5.Proteinprobes2.lonprobes6.DNAprobes三m23.NIRFFluorescenceimage7.Membraneprobes4.DNAsequence8.pHprobes20050705UORESCENCESPECTROSCOPFluorescenceresearch◆Keyword:FluorescenPeriod:Jan/2005-Jun/2005◆Database:美国化学会(ACS)数据库356篇WPS电子期刊全文数据库206篇>SD0s全文数据库(上交镜像)1890篇英国皇家化学会(RSC)数据库120篇研究与应用领域涉及:生命环境材料等三m2讲座要求了解:荧光探针表征应用研究意义20050705UORESCENCESPECTROSCOPMolecularProbeMolecularreporterFg-1.Fluorescentproberepresentsamolecularreporterinthe2biologicalsample.FromJFluorescence0050705aMolecularProbesMicrosoftInternetExplorerlecularprobeso3.38.D000地址①的t!/wphe囝转到链“快译国圃回圖图上网时手国·急的aBoutusProductsMandbookLiteratureGalleryResources(cmlactareerMbYv图B1Da550ysProbes/ewsvailableonline(viewNeiNItemMicrobiologyCatalogcfGenamicsandtMiclecularBiologyPreductsNewTheLeaderinfiuorescencetechnoloMicRobe:44GenomicsandSYERSafeDNAgelstain-TheroteInBlochemistry5eReactiveDyesandRecantAdditonstoCurpraductesecondaryDetectionB下SignalTransduction9SubscribetoprobesonlineTerharrJablonskidiagramVIbrationalrelax,VR,10-12-10-1sInternalconversionC,10-1110-13sLUMOintersystemcrossing.ISC,10-2.10-5s10-15T1hfluorescencehvE10-8svPhosphorescence氵lsc103-105hvHOMOUORESCENCESPECTROSCOPCharacteristicsofFluorophoreExcitationwavelength激发波长λaxEmissionwavelength发射波长enExtinction(absorption)coefficient吸光系数EStoke'sshiftFluorescencelifetime荧光寿命τFluorescencequantumyield荧光量子产率ΦFluorescenceAnisotropy各项异性r5fluorescencepolarizat光偏振p220050705UORESCENCESPECTROSCOPEXcitationandemissionspectraEXcitationspectrumEmissionspectra三m三20050705FluorescencelifetimesimplifiedJablonskidiagramrelaxation(10-12sT:Emissionratehv/DKnonradiativedecayrate●Definition●ExpressionAveragelifetime:theaveragetimethemoleculespendsintheexcitedstatepriortoreturntothegroundstate厂+kn7FluorescenceQuantumYieldDefiniti