药理学全英实验课件：第一次课Handing Animals and Administration

HandingAnimalsandAdministrationAnimals

Differentanimalsareusedinpharmacologicalexperiments.Commonlytheyarerabbits,ratsandmice.Rabbit

Rabbitsarecommonlyusedintheresearchoffever,cardiovasculardiseasesandinfectiousdiseases.Astheirbigeye-balls,theycanalsobeusedintheophthalmologicalresearches..Catchandhold

Thecorrectholding:

Raisetherabbitslightlybyholdingthenuchalskinwithonehand,andsupportthebuttockwiththeotherhand.

①and②Ifyouholdtherabbitwiththeback,itis

likelythatyouwillhurttheirkidneys.

③Ifyouholdtherabbitbytheirears,you

willhurttheirearsbecausetheearsare

tootendertosupporttheirweight.TheerrorholdingFixingarabbit

1.Weight2.Fixarabbitintherabbitbox.3.Anesthesiaofanimal4.Fixation

Laytherabbitonitsback.Thenfixtherabbitontheoperationplatformbybindingtheextremitiesanditsheadwithropesbyanotherperson.

Intravenousinjection

Weusuallyusetheveinoftheinnermarginoftheearasarouteofadministration.Intravenousinjection

Clipthefursontheveinwettheintendedpartwithwaterpresstheveinrootorfliptheveinlightlydotheinjectionfromtheremoteendofveinwithdrawtheneedleandstopbleedingwithgauzestepsmouse

Micearemostcommonlyusedanimalsinpharmacologicalexperiment.Theycanbeusedinthetoxicitytests,theresearchofphymatologyandendocrinology,etc.TonumbermouseLiesonbellyThesolutionofpicricacidisusedtonumberthemouse.Wenumberthemousefromtherightsidetotheleftside.HoldingamouseTherearetwometholdstoholdthemouse.Picture1Holdthemousewithtwohands.Beginnerscanusethismethod.Picture2Holethemousewithonehand.hand.This

metholdisrecommendedforskillfulpeople.(1)Holdthemousewithonehand.

Thismetholdisrecommendedforskillfulpeople.Catchthetailwithforefingerandthumb,clampthetailwithpalm,littlefingerandringfingerandthencatchtwoearsandbackskinwiththumbandforefinger.(2)Holdthemousewithtwohands.

Beginnerscanusethismethod.Firstly,catchthemouse'stailwithrighthandandputthemouseonaroughobjectatthesametime.Secondly,pinchtwoearsandheadskinwiththumb,forefinger

andmiddlefingeroflefthand.Lastly,clampthedorsalandtailskinwithringfinger,littlefinger.Makesureitsheadandneckstraight;payattentiontoitsbreath..AnintraperitonealinjectionInjectingplace:leftlowerquadrantPushtheneedleatanangleof30-40degrees.Youcansenseabreaktroughwhentheneedlepushthroughtheabdomimalmuscle.Thenadvancetheneedleforabout1.2cm.Rotateoutneedleafterinjection.AsubcutaneousinjectionInjectingplace:backPushtheneedleatanangleof30-40degrees.Tomakesuretheneedlecanbeflexibleundertheskin.Afterinjectionthereisalumponthesurfaceoftheskin.Rotateoutneedleafterinjection.AintramuscularinjectionInjectingplace:theoutsideoftheupperpartofthehindlimbPushtheneedleatanangleof30-40degrees.Thenadvancetheneedleforabout0.5cm.Afterinjectionthereisnolumponthesurfaceoftheskin.Rotateoutneedleafterinjection.OralinjectionInserttheneedlefromthecornerofthemouse.Presstheheadwiththeneedlesothattheoralcavityandesophagusformastraightpassage.Thenadvancetheneedleforabout2cm.Ifthereisresistance,withdrawtheneedleanddoitagainatadifferentangle.Afterinjection,withdrawtheneedlealongesophagus.Collectingbloodfromeyeball

Clipsomeofthebeard.Paintalittleheparinaroundtheeyesandonthereactionplate.Pulltheneckskinwiththethumbandsecondfingertothelefttomaketherighteyeballpopout.Inserttheneedlefromtheeyecornernearthenose.Stirtheneedletopuncturethevenousplexusbehindtheeyeball.Lifttheeyeballgentlytoletthebloodoutsmoothly.Pullouttheneedleimmediatelyandletgothemouse.Thebloodwillstopautomatically.stepsKillingamouse

Withtheleftthumbandsecondfingerpresshardthemouseneck,withtherighthandtakethetailandpullupatanangleof45degrees,sothatthemousemaydiefromdisjointoftheneck.RatRatscanbeusedintheresearchofmedica,cardiovascular

disease,oncology,etc.Ratsaremoredangerousthanmice.Youhavetowearacanvasgloveorcoveraclothontheratbeforeholdingarat.Sothatyouwon’tbebittenbythem.Weighingarat

Putthecagewiththeratinontheleftscalepanandtheweightsontherightscalepan.Readtheresultsafterbalance.Holdingarat

Ratsmaybeliftedbytheirtailswithrighthandandputontheroofofthecage.Wearcanvasgloveonyourlefthandorcovertherat’sheadandback.Grasptheskinoftheneckandtheback.Setitfirmlyinyourleftpalm.TheotherhandingsThehandingofratssuchastheintraperitonealinjection,thesubcutaneousinjection,theintramuscularinjectionandoralinjection,arejustlikethatofmice.Thedifferentthingisitisneedtwostudentstocollaboratewitheachother.Onestudentholdtherat,andtheotherstudentdothehandings.Requirements1.Rabbit:holding,intravenousinjection2.Rat: