中药药剂学课件：Peppermint Oil β-CD Complex and ointment

ExperimentforPharmaceuticsofTCMForgrade3,semester2ChinaPharmaceuticalUniversity

ChinaPharmaceuticalUniversityExercise1

CyclodextrinInclusionComplexes——PeppermintOilβ-CDComplex

αβγ

6

7

8ChinaPharmaceuticalUniversityIntroductionWhatareCyclodextrins(CDs)?

ChinaPharmaceuticalUniversityWhatisthestructureoftheβ-CD?β-CDaremolecularcapsuleswithahydrophobicinsideandahydrophilicoutside.-OH-O--OHIntroduction

ChinaPharmaceuticalUniversityWhataretheinclusioncomplexes?MoleculesofappropriatesizeandshapefitintotheCDcavitytoformtheinclusioncomplexes.GuestmoleculesHostmoleculesSolidmoleculesLiquidmoleculesGaseousmoleculesIntroduction

ChinaPharmaceuticalUniversityHowtoproducecyclodextrininclusioncomplexes?(1)Saturatedwatersolution/Co-precipitation/Re-crystallizationMethod(2)

Grinding/kneadingMethod(3)

Freeze-dryingMethod(4)

SpraydryingMethod(5)

UltrasonicMethodIntroduction

β-CD8g

InclusionCompound

Peppermintoil

2mL

APIWater

16mL

solvent

ChinaPharmaceuticalUniversityFormulation

β-CD+16mL

water

addpeppermintoil

vacuumfiltrationWashtheresiduewith5ml95%

ethanolDrytheinclusioncomplexesat

40℃Storetheproductinadesiccator

MixingGrindingfor30minChinaPharmaceuticalUniversitySection1:Processpeppermintoil2mL95%Ethanol2mL+

ChinaPharmaceuticalUniversityIdentificationMethodHowtoidentifythecyclodextrininclusioncomplexes?(1)x-raydiffractometry(2)Differentialthermalanalysis/Differentialscanningcalorimetry(3)Microscopicexamination(4)FluorescentSpectrometry(5)TLC(6)UV,IR,NMR,HPLC,GC,etal

1.Solutionpreparation

Thesample:0.5ginclusioncomplexes+

2mL95%

ethanol

ultrasonicationfor30minthesupernatantasthetestsolution

Thereference:2dpeppermintoil+

2mL95%

ethanol

2.TLCIdentification

Applyseparatelytotheplate2μLofthesamplesolutionandreferencesolution.

Developingsolvent:acetyl-petroleum(15:85,V/V)

Colordeveloping:drytheplateinair,spraywitha1%solutionofvanillaldehydeinsulphuricacidandheatuntilthespotsaredistinct.

ChinaPharmaceuticalUniversitySection2:Identification

ChinaPharmaceuticalUniversityExercise2:Ointment——UnguentumGeranii(CommonHeron’sBillOintment)

ChinaPharmaceuticalUniversityWhatareointments?Ointmentsarepaste-likepreparationswithasuitableconsistency,usuallycontainingmedicaments,crudedrugsandtheirextractsincorporatedinsuitablebase,andintendedforexternalapplicationtotheskin.Thetypesoftheointments(1)Fattybases(2)Watersolublebases(3)EmulsifyingbasesIntroduction

HerbaErodiiseuGeranii

50g

API

Woolfat2.5g

base

Ethylp-hydroxybenzoate

0.015g(2d)preservative

Vaseline

Q.S

base

Makeupto

50g

ChinaPharmaceuticalUniversityFormulation

Section1:ExtractionHerbaErodiiseuGeranii

extract

50ml

Standfor7dsupernatantConcentrateto3-4gSection2:OintmentPreparation

Theextract

+

Ethylp-hydroxybenzoate+

Woolfat+Vaseline

mixthoroughlyandth