免疫浊度测定及其临床应用课件

免疫浊度测定及其临床应用1免疫测定分类免疫浊度测定

g~

ng标记免疫测定ng~

pg免疫浊度测定免疫沉淀反应海德堡曲线Heidelberg’s

Curve抗原浓度测定范围抗体过剩抗原过剩Ag

*Ab免疫浊度测定分类透射浊度法散射浊度法透射浊度法和散射浊度法的光路检测器A(透射浊度法)检测器B(散射浊度法)θI0I浑浊样品光源散射浊度法AnalyteAntibodyOther

stuffAnalyte-antibody

complexingNow

big

enough

to

scatter

lightToo

small

to

detect

opticallyWhat

happens

inside

the

flowcell?31ScatterScatter

Signal

Versus

TimeNephelometry1.

Buffer

additionTime2.

Sample

Addition3.

Antibody

additionFluid

in

the

cell

has

causes

little

scatter2Adding

sample

increases

scatter

slightlyAdding

antibody

causes

a

largeincrease

in

light

scattering免疫浊度测定分类2终点法速率法终

点

法固定时间法速率散射浊度法速率峰的测定速率峰的测定ScatterMeasure

the

RATE

After

Antibody

AdditionRate

Nephelometry321Time1.

Buffer

addition 2.

Sample

Addition 3.

Antibody

additionFirst

derivative:

rate

of

change

of

scatter

over

time;this

is

the

SLOPE

of

thecurveRatePEAKRATETIMERATERate

of

Change

of

Scatter

Signal

versus

TimeCONCENTRATIONRATE在速率峰基础上完成的标准曲线抗原过剩的测定Analyte-antibody

complexingAnalyteAntibodyAntigen

ExcessRATEofSCATTER抗体过量时的反应抗原过量时的反应60085反应时间(秒)ARRAY抗原过量自动检测加入校正液药物浓度测定免疫浊度抑制测定(小分子物质)12Conjugate-antibody

complexing213Inhibition

of

complexing

by

hapten

(drug)Conjugate

(hapten

on

carrier)AntibodyHapten

(drug)免疫浊度测定试剂抗体试剂的要求1特异性2亲和力3反应时保持抗体过剩455测定范围应包括高于及低于正常值的50%测定的精密度应在5%左右校正曲线：聚乙二醇的增强作用抗原浓度C1C2C3吸光度4%聚乙二醇不加聚乙二醇试剂组成配方举例~

8.2试剂1100mmol Tris或磷酸缓冲液

pH7.24% 聚乙二醇(分子量6000或10000)1%非离子型表面活性剂150mmol氯化钠或硫酸钠0.1

g/l

叠氮钠~

8.2试剂2100mmol Tris或磷酸缓冲液

pH7.220% 多克隆羊抗血清150mmol氯化钠0.1

g/l

叠氮钠抗原浓度测定范围抗体过剩抗原过剩Ag

*Ab技术特性并行的检测系统:Rate

NIPIARate

NephelometryNIPIA：近红外颗粒速率法免疫分析BenefitsAbility

to

expand

the

menuinto

high

sensitivity

assaysincluding:

Ferritin,

Digoxin,

and

IgE.NIR

eliminates

the

interferenceof

biological

substancessuch

as

biliribin

andhemoglobin.NIPIA：近红外颗粒速率法免疫分析优点扩展检测菜单，增加高敏项目分析：

如铁蛋白，地高辛 ，IgE，超敏CRP等近红外波长分析消除生物物质如胆红素和血色素的干扰NIR：940nm波长近0oC检测角检测颗粒大小：200~500nm直径速率法测定NIPIA：940nmLED光源速率散射法：670nm激光光源蛋白应用分类心血管功能监测载脂蛋白A1载脂蛋白B脂蛋白a11、最灵繁的人也看不见自己的背脊。、最灵繁的人也看不见自己

的背脊。————非洲非洲22、最困难的事情就是认识自己。、最困难的事情就是认识自己。————希腊希腊33、有勇气承担命运这才是英雄好汉。、有勇气承担命运这才是英

雄好汉。————黑塞黑塞44、与肝胆人共事，无字句处读书。、与肝胆人共事，无字句处读