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TrendsandNeedsinFoodDiagnosticsLarissaKahr-BIT,AustriaMalinBrännback-TEKES,FinlandGabrielavonBlankenfeld-Enkvist-TEKES,FinlandTrendsandNeedsinFoodDiagnAimoftheStudyInvestigatetechnologicaltrendsandneedsinFoodDiagnosticsUnderstandthefactorsthatshapeandinfluencethedevelopmentofthisfield.BureauforInternationalResearchandTechnologyCooperation-AustriaAimoftheStudyInvestigatete2FocusBackgroundonfoodsafetymanagementtoolsDescriptionofthemethodscurrentlyusedandnewmethodsdevelopedThemarketforfooddiagnostics

BureauforInternationalResearchandTechnologyCooperation-AustriaFocusBackgroundonfoodsafety3DefinitionsFoodDiagnosticsappliesmodernmethodsdevelopedinhumaninvitrodiagnostictofoodRapidMethodscomprisesdifferenttypesoftestsMiniaturisedbiochemicalkitsAntibody-basedtestsNucleicacidhybridisation-basedtestsManual,semi-automatedorfullyautomated

BureauforInternationalResearchandTechnologyCooperation-AustriaDefinitionsFoodDiagnosticsap4DetectionBacteria,viruses,parasites,chemicals,biotoxins,heavymetalsandprionsInallstepsofthefoodchain-fromrawmaterialstoendproducts

BureauforInternationalResearchandTechnologyCooperation-AustriaDetectionBacteria,viruses,pa5FoodastestmatrixExtremelyvariedinitschemicalcompositionContainsmanydifferentingredientsMightcontainanintrinsicmicrobialfloraBureauforInternationalResearchandTechnologyCooperation-AustriaFoodastestmatrixExtremelyv6FrameFoodSafetyProcesscontrolismoreefficientthanproductcontrolThesafetyofaproductwithlowdefectratescannotbeguaranteedthroughsamplingandtestingThefocusischangingfromend-producttestingtoHACCPThishastobeconsideredwhendevelopingnewmethodswithintheconceptualframeoffoodsafetyBureauforInternationalResearchandTechnologyCooperation-AustriaFrameFoodSafetyProcesscontr7Microbiologicalmethodscanbedividedinto“traditional”or“conventional”methodsandrapidmethods

BureauforInternationalResearchandTechnologyCooperation-AustriaMicrobiologicalmethodscanbe8ConventionalMethodsConventionalmethodsrelyonculturingmicroorgansimsontoagarplatesLaboriousandtime-consuming84%ofalltestsarebasedoncountingviablecellsRequiredbynationalandinternationalregulatoryagenciesforofficialcontrolBureauforInternationalResearchandTechnologyCooperation-AustriaConventionalMethodsConvention9RapidMethodsAdvantagesinanalysistimePossibilitytoeliminatelabourintensivestepsPotentialforautomationHowever,lengthyincubationproceduresarestillnecessaryFirstone-shiftmethodsunderdevelopmentELISAandPCRaredominatingLackofacommonvalidationprocedureMostlynotacceptedforofficialcontrolpurposesBureauforInternationalResearchandTechnologyCooperation-AustriaRapidMethodsAdvantagesinana10MethodsAdvancesinViableCellCountMethodsMiniaturisationandDiagnosticKits,BiochemicalIdentificationTechniquesAntibody-basedmethodsImmunomagneticSeparationNucleicacid-basedassaysBiosensorsMicroarraysFlowCytometryBacteriophage-BasedTechniquesATPbioluminescenceAdenylatekinasebioluminescenceRiboprintingandPulse-FieldGelElectrophoresis

BureauforInternationalResearchandTechnologyCooperation-AustriaMethodsAdvancesinViableCell11Viablecellcounting

Willremainimportantfortheassessmentofsafetyandqualityoffoodproductsdevelopmentofsemi-automatedalternativemethodsnewmethodsarebasedongrowthandmetabolicactivityMicroscopicmethodsflowcytometryandcellsorting(FACS)BureauforInternationalResearchandTechnologyCooperation-AustriaViablecellcounting

Willrema12Immunoassays

ELISA(withdipsticktechnology),ImmunochromatographyFullyautomatedsystemsalreadyonthemarketNewrecombinantantibodiesandmolecularimprintingtechniquestoimprovesensitivityandversatilityBureauforInternationalResearchandTechnologyCooperation-AustriaImmunoassays

ELISA(withdipst13ImmunomagneticSeparation

SpeedupenrichmentstepsNodetectionmethoditselfCombinationwithdifferentend-detectionmethodstoimprove -Speedandsensitivity -Samplethroughput -Userfriendliness -AutomationexpectedinthenextfutureBureauforInternationalResearchandTechnologyCooperation-AustriaImmunomagneticSeparation

Spe14Nucleicacidbasedassay

DirectlyorafteramplificationwithPCR

DevelopmentofhomogenousassaysandautomationimprovesroutineuseandreducesfalsepositiveresultsafterPCR

NewassayformatsunderdevelopmentBureauforInternationalResearchandTechnologyCooperation-AustriaNucleicacidbasedassay

Direc15BiosensorsandMicroarraysOn-lineoron-site,sensitive,low-cost,rapidmethodsforroutine-usePrototypesunderdevelopmentHavehighpotentialforautomationSimpleandportableequipmentcanbedesignedApplicationswithinqualityandprocesscontrol,controloffermentationprocesses,qualityandsafetycontrolofrawmaterialsandforHACCPmonitoring

BureauforInternationalResearchandTechnologyCooperation-AustriaBiosensorsandMicroarraysOn-l16Hygienemonitoring

NeedtodeveloprealtimemethodstomonitorcleaningprocedureMethodsshouldbecheap,robustATPbioluminescence,Adenylatekinase,biosensorapplicationsmightbefurtherdevelopedBureauforInternationalResearchandTechnologyCooperation-AustriaHygienemonitoring

Needtodev17MethodsImplementation

InhousealternativemethodsforviablecellcountshygienemonitoringchemicalanalysisOutsourcingpathogentestingBureauforInternationalResearchandTechnologyCooperation-AustriaMethodsImplementation

Inhous18TheMarketforFoodDiagnostics

DefinitionsFoodAnalysis:allfoodtestingFoodDiagnostics:foodtestingwithrapidmethodsMicrobiologicalTesting:alltestingformicroorganismsFoodMicrobiologicalTesting:alltestingformicroorganismsinfoodandbeveragesBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnostic19TheMarketforFoodDiagnostics

SomefiguresFoodanalysisglobalmarket€1.1billionSharedbyabout50companiesUSAandEuropemakeupabout2/3€565millionglobalsalesforFoodmicrobiologicaltestsRapidmethods€115millionora20%totalmarketshareBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnostic20

TechnologySales($USmillions)MarketShare(%)ELISA/EIA7061DNA2522Impedance109FlowCytometry54ATP54Total115100BureauforInternationalResearchandTechnologyCooperation-Austria

TechnologySales($USmillions21

FoodAnalysis(€million)

FoodDiagnostics(€million)%FoodDiagnostics(€million)Foodpathogens97,51315%Spoilage22037,517%Chemicalcontaminants5319,540%Processchemicals1201610%Total490,586

BureauforInternationalResearchandTechnologyCooperation-Austria

Foodpathogens97,51315%Spoil22Validation

AOACvalidationbodyintheU.S.

NordValandMicroValaretheNordicandEuropeanbodies

Problem:lackofacommonvalidationprocedureInhibitsestablishmentofrapidmethods

BureauforInternationalResearchandTechnologyCooperation-AustriaValidation

AOACvalidationbod23ConclusionsManynewmethodsandtechnologiesarebeingdevelopedIncreasedsamplethroughputandefficiencythroughautomationThemarketforrapidmethodsissmallPenetrationofrapidmethodshasbeenslowLackofacommonvalidationprocedureinhibitsacceptanceofnewmethodsTheincentivetotransfertechnologiesmightbetoolowBureauforInternationalResearchandTechnologyCooperation-AustriaConclusionsManynewmethodsan24RecommendationsMicroarraysandbiosensors

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