甘蓝型油菜BnaWOX45基因在拟南芥中的功能研究

REF_Ref164945318\r\h[55]。我们对BnaWOX45超量表达转基因拟南芥植株和野生型拟南芥的活性氧染色结果表明，活性氧的积累在BnaWOX45超量表达转基因拟南芥植株中显著增加，尤其是在主根和侧根的根尖区域。这一现象暗示了BnaWOX45基因可能通过调节根尖区域的活性氧水平来影响细胞的分化过程，从而影响根的生长。虽然这种作用机制的细节还未完全明了，但这一发现为进一步探究该基因如何控制植物根的生长提供了新的线索。6结论本研究对BnaWOX45基因功能进行了初步探究，具体结论如下：超量表达转基因拟南芥根长表型实验表明，BnaWOX45基因能够抑制植物主根的生长，并且这一效应受到磷缺乏信号的影响。根尖淀粉粒和活性氧染色结果显示，BnaWOX45基因通过降低根尖分生组织中干细胞的分化程度来负向调控主根的伸长。

参考文献Salazar-HenaoJE,Vélez-BermúdezIC,SchmidtW.Theregulationandplasticityofroothairpatterningandmorphogenesis.Development,2016,143(11):1848-1858.MayerKF,SchoofH,HaeckerA,LenhardM,JürgensGL.TheRoleofWUSCHELinregulatingstemcellfateintheArabidopsisshootmeristem[J].Cell,1998,95,805.冯珊珊,王璐,周益等.WOX家族基因调控植物生长发育和非生物胁迫响应的研究进展[J].生物技术通报,2023,39(05):1-13.IkedaM,MitsudaN,Ohme-TakagiM.ArabidopsisWUSCHELisabifunctionaltranscriptionfactorthatactsasarepressorinstemcellregulationandasanactivatorinfloralpatterning［J］.PlantCell,2009,21（11）:3493-3505.vanderGraaffE,LauxT,RensingSA.TheWUShomeobox-containing（WOX）proteinfamily［J］.GenomeBiol,2009,10（12）:248.FisherK,andTurnerS.PXY,areceptor-likekinaseessentialformaintainingpolarityduringplantvascular-tissuedevelopment[J].Curr.Biol,2007,17:1061–1066.KawaiT,ShibataK,AkahoshiR,NishiuchiS,TakahashiH,NakazonoM,KojimaT,Nosaka-TakahashiM,SatoY,ToyodaA,Lucob-AgustinN,Kano-NakataM,SuraltaRR,NionesJM,ChenY,SiddiqueKHM,YamauchiA,InukaiY.WUSCHEL-relatedhomeoboxfamilygenesinricecontrollateralrootprimordiumsize.ProcNatlAcadSciUSA.2022Jan4;119(1):e2101846119.HirakawaY,KondoY,andFukudaH.TDIFpeptidesignalingregulatesvascularstemcellproliferationviatheWOX4homeoboxgeneinArabidopsis[J].PlantCell,2010,22:2618–2629.FletcherJC,BrandU,RunningMP,SimonR,MeyerowitzEM.SignalingofcellfatedecisionsbyCLAVATA3inArabidopsisshootmeristems[J].Science,1999,283,1911−1914HamantO,HeislerMG,JonssonH,KrupinskiP,UyttewaalM,BokovP,CorsonF,SahlinP,BoudaoudA,MeyerowitzEM.DevelopmentalpatterningbymechanicalsignalsinArabidopsis[J].ScienceSignaling,2008,322(5908).1650.BreuningerH,RikirschE,HermannM,etal.DifferentialexpressionofWo×genesmediatesapical-basalaxismationintheArabidopsisembryo[J].revCell,2008,14(6):867-876.LiuJ,ShengL,XuY,LiJ,YangZ,HuangH,XuL.WOX11and12areinvolvedinthefirst-stepcellfatetransitionduringdenovorootorganogenesisinArabidopsis.PlantCell.2014Mar;26(3):1081-93.IkedaM,andOhme-TakagiM.ArabidopsisWUSCHELisabifunctionaltranscriptionfactorthatactsasarepressorinstemcellregulationandasanactivatorinfloralpatterning[J].PlantCell,2009,21,3493–3505.SarkarAK,LuijtenM,MiyashimaS,LenhardM,HashimotoT,NakajimaK.,etal.ConservedfactorsregulatesignallinginArabidopsisthalianashootandrootstemcellorganizers.Nature,2007,446,811–814.PeralesM,ReddyGV.Stemcellmaintenanceinshootapicalmeristems[J].CurrOpinPlantBiol,2012,15,10−16.ShinoharaH,MatsubayashiY.ReevaluationoftheCLV3-receptorinteractionintheshootapicalmeristem:dissectionoftheCLV3signalingpathwayfromadirectligand-bindingpointofview[J].PlantJ,2015,82,328−336.ChengS,HuangY,ZhuN,etal.ThericeWUSCHEL-relatedhomeoboxgenesareinvolvedinreproductiveorgandevelopment,hormonesignalingandabioticstressresponse[J].Gene,2014,549(2):266-274.Minh-ThuPT,KimJS,ChaeS,JunKM,LeeGS,KimDE,CheongJJ,SongSI,NahmBH,KimYK.AWUSCHELHomeoboxTranscriptionFactor,OsWOX13,EnhancesDroughtToleranceandTriggersEarlyFloweringinRice.MolCells.2018Aug31;41(8):781-798.ZhuJ,ShiH,LeeBH,DamszB,ChengS,StirmV,ZhuJK,HasegawaPM,BressanRA.AnArabidopsishomeodomaintranscriptionfactorgene,HOS9,mediatescoldtolerancethroughaCBF-independentpathway.ProcNatlAcadSciUSA.2004Jun29;101(26):9873-8.ZhaoY,HuY,DaiM,HuangL,ZhouDX.TheWU-SCHEL-relatedhomeoboxgeneWOX11isrequiredtoac-tivateshoot-bornecrownrootdevelopmentinrice[J].PlantCell,2009,21,736−748.段陈兆波，余健.我国油菜生产形势分析及科研对策研究［J］.中国油料作物学报，2010，32（2）：303-308.徐子先.甘蓝型油菜氮效率评价及其差异的生理机制探究［D］.北京：中国农业科学院，2017．韩配配，秦璐，李银水等.不同营养元素缺乏对甘蓝型油菜苗期生长和根系形态的影响［J］.中国油料作物学报，2016，38（1）：88-97.张哲，殷艳，刘芳等.我国油菜多功能开发利用现状及发展对策［J］.中国油料作物学报，2018，40（5）：618-623.PostmaJA,SchurrU,FioraniF.Dynamicrootgrowthandarchitectureresponsestolimitingnutrientavailability:linkingphysiologicalmodelsandexperimentation[J].BiotechnologyAdvances,2014,32(1):53-65.DunbabinV,RengelZ,DiggleAJ.Simulatingformandfunctionofrootsystems:efficiencyofnitrateuptakeisdependentonrootsystemarchitectureandthespatialandtemporalvariabilityofnitratesupply[J].Functionalecology.2004.18(2).204-211.陈金慧,王鹏凯,张艳娟等.植物根尖分生组织干细胞调控模式［J］.南京林业大学学报(自然科学版),2012,36(04):127-132.ForzaniC,AichingerE,SornayE,etal.WOX5SuppressesCYCLINDActivitytoEstablishQuiescenceattheCenteroftheRootStemCellNiche[J].CurrentBiology,2014,24(16):1939-1944.HelariuttaY,FukakiH,Wysocka-DillerJ,NakajimaK,JungJ,SenaG,HauserMT,BenfeyPN.TheSHORT-ROOTgenecontrolsradialpatterningoftheArabidopsisrootthroughradialsignaling.Cell.2000May26;101(5):555-67.LevesqueMP,TevaV,WolfgangB,etal.Correction:Whole-GenomeAnalysisoftheSHORT-ROOTDevelopmentalPathwayinArabidopsis[J].PLoSBiology,2006,4(7):e249.SabatiniS,HeidstraR,WildwaterM,ScheresB.SCARECROWisinvolvedinpositioningthestemcellnicheintheArabidopsisrootmeristem[J].Genes,2003,Dev17:354–358.GalinhaC,HofhuisH,LuijtenM,WillemsenV,BlilouI,HeidstraR,etal.PLETHORAproteinsasdose-dependentmasterregulatorsofArabidopsisrootdevelopment[J].Nature,2007,449:1053-1057.CausierB,AshworthM,GuoW,DaviesB.TheTOPLESSInteractome:AFrameworkforGeneRepressioninArabidopsis[J].PlantPhysiology,2012,158,423–438.MeyerMR,ShahS,ZhangJ,etal.EvidenceforIntermolecularInteractionsbetweentheIntracellularDomainsoftheArabidopsisReceptor-LikeKinaseACR4,ItsHomologsandtheWox5TranscriptionFactor[J].PLOSONE,2015,10(3):e0118861.Takai,K.TheNitrogenCycle:ALarge,Fast,andMystifyingCycle.MicrobesEnviron.Vol.34,No.3,223-225,2019.田丽华.氮对作物生长发育的影响及其施肥方法[J].河北农业科技,2008,No.342(15):40.HerbertJ.Kronzucker,M.YaeeshSiddiqi,AnthonyD.M.Glass.Coniferrootdiscriminationagainstsoilnitrateandtheecologyofforestsuccession[J].Nature:Internationalweeklyjournalofscience,1997,385(6611).JiaZ,vonWirénN.Signalingpathwaysunderlyingnitrogen-dependentchangesinrootsystemarchitecture:frommodeltocropspecies.JExpBot.2020;71(15):4393-4404.HansMotte,SteffenVannesteandTomBeeckman1.MolecularandEnvironmentalRegulationofRootDevelopment.AnnualReviewofPlantBiology,2019.70:22.1–22.24WangJ,QinQ,PanJ.

etal.

Transcriptomeanalysisinrootsandleavesofwheatseedlingsinresponsetolow-phosphorusstress.

ScientificReport,2019,

9,19802.李万梅.作物缺素症状简介［J］.青海农技推广,2012,No.65(02):46+45.汪翠莲.农作物常见缺磷诊断及防治措施［J］.农技服务,2008,No.238(07):61+63.ZhouM,ZhuS,MoX,GuoQ,LiY,TianJ,LiangC.ProteomicAnalysisDissectsMolecularMechanismsUnderlyingPlantResponsestoPhosphorusDeficiency.Cells.2022;11(4):651.RichardsonA.E.“Soilmicroorganismsandphosphorusavailability,”inSoilBiota:ManagementinSustainableFarmingSystems,edsV.V.S.R.GuptaandE.R.Grace(Melbourne,VIC:CSIROPublishing),1994,50–62.MüllerJ,ToevT,HeistersM,TellerJ,MooreKL,HauseG,DineshDC,BürstenbinderK,AbelS.Iron-dependentcallosedepositionadjustsrootmeristemmaintenancetophosphateavailability.DevCell.2015Apr20;33(2):216-30.GruberBD,GiehlRF,FriedelS,vonWirénN.PlasticityoftheArabidopsisrootsystemundernutrientdeficiencies.PlantPhysiol.2013Sep;163(1):161-79.WangX,WangZ,ZhengZ,etal.GeneticDissectionofFe-DependentSignalinginRootDevelopmentalResponsestoPhosphateDeficiency.PlantPhysiol.2019Jan;179(1):300-316.Gutiérrez-AlanísD,Yong-VillalobosL,Jiménez-SandovalP,etal.PhosphateStarvation-DependentIronMobilizationInducesCLE14ExpressiontoTriggerRootMeristemDifferentiationthroughCLV2/PEPR2Signaling.DevCell.2017Jun5;41(5):555-570.e3谢德体,蒋先军,王昌全.土壤肥料学[M].中国林业出版社,2015;15-18．SarkarAK,LuijtenM,MiyashimaS,LenhardM,HashimotoT,NakajimaK,ScheresB,HeidstraR,LauxT.ConservedfactorsregulatesignallinginArabidopsisthalianashootandrootstemce