菌藻共生系统中胞外聚合物与环丙沙星作用机制研究

-[36]。图3.8CANON和ABS提取的EPS与CIP相互作用红外光谱结论本论文研究了菌藻共生系统内胞外聚合物（EPS）跟环丙沙星（CIP）的作用机制，依靠多维度实验进行分析探究，得出了以下结论：就去除效果这个层面而言，菌藻共生污水处理系统彰显出高效的CIP去除能力，EPS在系统中发挥核心功效，实验结果显示，菌藻共生系统中的EPS可采用吸附、络合等多种方式和环丙沙星发生相互作用，EPS的多糖、蛋白质等成分依靠着氢键、静电吸引及配位键等作用力，明显带动了环丙沙星的去除，和单一微生物体系比，菌藻共生体系的EPS分泌量更高，这增添了对环丙沙星的协同去除能力，给抗生素污染治理带来了新途径。机制研究证实，EPS中的多糖、蛋白质等物质跟CIP分子之间存在多重相互作用，FTIR以及3D-EEM分析显示，EPS中的羟基、羧基等官能团跟CIP的羰基等发生氢键结合及静电吸附作用；微生物代谢产生的活性基团让CIP出现部分氧化降解，二者协同配合工作，达成对CIP的有效去除。和传统处理技术相比，菌藻共生污水处理系统跟EPS作用相耦合具有明显优势，此系统无需额外添加碳源，用光能推动自养代谢维持系统的运行，既符合绿色低碳要求，又具经济可行性；且菌藻共生体系具有自我修复与再生的本领，把处理周期给延长了，减少运行开支，在抗生素污染治理领域展示出不错的应用潜力。参考文献刘洋.微藻及其胞外聚合物对水中喹诺酮抗生素去除途径的研究[D].西北农林科技大学,2024.张焕军,王席席,李轶.水体中抗生素污染现状及其对氮转化过程的影响研究进展[J].环境化学,2022,41(4):1168-1181.CaoDQ,YangWY,WangZ,etal.Roleofextracellularpolymericsubstanceinadsorptionofquinoloneantibioticsbymicrobialcellsinexcesssludge[J].ChemicalEngineeringJournal,2019,370:684-694.蔡意祥.环丙沙星在厌氧和好氧活性污泥上的吸附降解研究[D].湖南农业大学,2017.KartalB,KuenenJG,VanLoosdrechtMCM.Sewagetreatmentwithanammox[J].Science,2010,328(5979):702-703.LacknerS,GilbertEM,VlaeminckSE,etal.Full-scalepartialnitritation/anammoxexperiences–anapplicationsurvey[J].Waterresearch,2014,55:292-303.巫小丹,阮榕生,王辉,等.菌藻共生系统处理废水研究现状及发展前景[J].环境工程,2014,32(3):34-37.AlavianghavaniniA,ShayestehH,BahriPA,etal.Microalgaecultivationfortreatingagriculturaleffluentandproducingvalue-addedproducts[J].ScienceofTheTotalEnvironment,2024,912:169369.张萌,曹瑞瑞,肖唐佳,等.菌藻共生系统去除废水抗生素机理及相关研究进展[J].化肥设计,2025,63(01):11-14.应璐瑶,王荣昌.菌藻共生系统削减抗生素类污染物的去除途径及胁迫响应[J].化工进展,2023,42(1):469-479.MathaiS,VeghteD,KovarikL,etal.Opticalpropertiesofindividualtarballsinthefreetroposphere[J].Environmentalscience&technology,2023,57(44):16834-16842.ZhaoW,YouJ,YinS,etal.Extracellularpolymericsubstances-antibioticsinteractioninactivatedsludge:areview[J].EnvironmentalScienceandEcotechnology,2023,13:100212.宋悦,魏亮亮,赵庆良,等.活性污泥胞外聚合物的组成与结构特点及环境行为[J].环境保护科学,2017,43(2):35-40.柯楠,倪莹莹,何嘉淇,等.微生物胞外聚合物引起的金属腐蚀的研究进展[J].中国腐蚀与防护学报,2023,44(2):278-294.魏东.短程硝化反硝化工艺过程控制及N2O释放研究[D].山东大学,2016.任恬.藻菌共生系统中胞外聚合物提取条件优化及定量分析[D].华中农业大学,2024.袁宇杰,杨英,储明,等.胞外聚合物对重金属及抗生素吸附研究进展[J].水处理技术,2022,48(05):24-28.JiangHL,TayJH,YuL,etal.Ca2+augmentationforenhancementofaerobicallygrownmicrobialgranulesinsludgeblanketreactors[J].BiotechnologyLetters,2003,25(2):95-99.翟方帅.全程自养-菌藻共生生物膜系统构建及脱氮除磷性能研究[D].济南大学,2024.张宏扬.基于紫外三维荧光光谱的TOC检测关键技术研究[D].燕山大学,2020.XuC,WeiH,MengqingF,etal.Novelinsightsintosourceapportionmentofdissolvedorganicmatterinaquiferaffectedbyanthropogenicgroundwaterrecharge:Applicabilityofend-membermixinganalysisbasedopticalindices[J].TheScienceofthetotalenvironment,2022,863160885-160885.陈唐冬.傅里叶变换红外光谱(FTIR)技术在鉴别低温致死小鼠中的应用研究[D].中国人民解放军空军军医大学,2024.ChenW,WesterhoffP,LeenheerJA,etal.Fluorescenceexcitation−emissionmatrixregionalintegrationtoquantifyspectrafordissolvedorganicmatter[J].Environmentalscience&technology,2003,37(24):5701-5710.ShengG,YuH.Characterizationofextracellularpolymericsubstancesofaerobicandanaerobicsludgeusingthree-dimensionalexcitationandemissionmatrixfluorescencespectroscopy[J].WaterResearch,2006,40(6):1233-1239.AdavSS,LeeDJ.Extractionofextracellularpolymericsubstancesfromaerobicgranulewithcompactinteriorstructure[J].Journalofhazardousmaterials,2008,154(1-3):1120-1126.YamashitaY,TanoueE.Chemicalcharacterizationofprotein-likefluorophoresinDOMinrelationtoaromaticaminoacids[J].MarineChemistry,2003,82(3-4):255-271.BakerA,InverarityR.Protein‐likefluorescenceintensityasapossibletoolfordeterminingriverwaterquality[J].HydrologicalProcesses,2004,18(15):2927-2945.孙雅萍.基于硫铁矿自养反硝化的城镇尾水溶解性有机氮去除特性研究[D].济南大学,2024.ZhangT,LuJ,MaJ,etal.FluorescencespectroscopiccharacterizationofDOMfractionsisolatedfromafilteredriverwaterafterozonationandcatalyticozonation[J].Chemosphere,2008,71(5):911-921.XuJ,ShengG,MaY,etal.Rolesofextracellularpolymericsubstances(EPS)inthemigrationandremovalofsulfamethazineinactivatedsludgesystem[J].WaterResearch,2013,47(14):5298-5306.PapadopoulouA,GreenRJ,FrazierRA.InteractionofFlavonoidswithBovineSerumAlbumin: AFluorescenceQuenchingStudy[J].JournalofAgriculturalandFoodChemistry,2005,53(1):158-163.HuY,LiuY,XiaoX.InvestigationoftheInteractionbetweenBerberineandHumanSerumAlbumin[J].Biomacromolecules,2009,10(3):517-521.WeiD,WangB,NgoHH,etal.Roleofextracellularpolymericsubstancesinbiosorptionofdyewastewaterusingaerobicgranularsludge[J].BioresourceTechnology,2015,185:14-20.ZhangH,JiaY,KhanalSK,etal.UnderstandingtheRoleofExtracellularPolymericSubstancesonCiprofloxacinAdsorptioninAerobicSludge,AnaerobicSludge,andSulfate-ReducingBacteriaSludgeSystems[J].EnvironmentalScience&Technology,2018,52(11):6476-6486.YanL,LiuY,WenY,etal.Roleandsignificanceofextracellularpolymericsubstancesfromgranularsludgeforsimultaneousremovaloforganicmatterandammonianit