钮灰蝶线粒体基因组测序及系统发育分析

[9]，产生此现象的原因仍需进一步研究。控制区中出现了类似微卫星的重复序列，这与大多数鳞翅目昆虫的控制区相同。本文基于13个PCGs数据集对钮灰蝶进行BI和ML分析，结果均支持钮灰蝶是熏衣琉璃灰蝶以及大紫琉璃灰蝶的姐妹群,且都属于利灰蝶亚族。本研究丰富了灰蝶科线粒体基因组信息，为蝴蝶的物种鉴定提供新的分子标记，为解决灰蝶科各级阶元的系统发育关系提供了分子依据。如需进一步探讨钮灰蝶属和琉璃灰蝶属的系统发育关系，未来研究需进一步扩大取样范围，并补充更多的线粒体基因组序列数据。参考文献魏书军,陈学新.昆虫比较线粒体基因组学研究进展[J].应用昆虫学报,2006,48(6):1573–1585.CameronSL.Insectmitochondrialgenomics:implicationsforevolutionandphylogeny[J].TheAnnualReviewofEntomology,2014,59(1):95–117.BooreJL.Animalmitochondrialgenomes[J].NucleicAcidsResearch,1999,27(8):1767–1780.NassMMK,NassS.IntramitochondrialfiberswithDNAcharacteristics[J].CellBiology,19(3):593–611.SalvtoP,SimonatoM,BattistiA,NegrisoloE.Thecompletemitochondrialgenomeofthebag-sheltermothOchrogasterlunifer(Lepidoptera,Notodontidae)[J].BMCGenomics,2008,9(1):1.王备新,杨莲芳.线粒体DNA序列特点与昆虫系统学研究[J].昆虫知识,2002,39(2):88–92.何海燕,余伟东,蒋韦斌.蝶类线粒体基因组学研究进展[J].生命科学,2016,29(7):978–985.赵乐.三种直翅目昆虫全长转录组和线粒体转录组分析[D].陕西:陕西师范大学,2018.张兰兰.五种灰蝶线粒体基因组比较研究及其系统发生分析[D].安徽：安徽师范大学,2013.StradomskyB.V.,YakovlevR.V.,FominaE.S.MoleculargeneticconfirmationofthesubspeciesstatusofBlues[J].RussianEntomologicalJournal,2019,28(2):186–188.李景光.深圳市蝴蝶资源调查[D].广东：华南农业大学,2007.夏雪秦,陈晓,夏琛琛,石庆会,郝家胜.基于线粒体CO1和CYTB基因的中国灰蝶三亚科主要类群间的系统发育关系分析[J].昆虫学报,2016,59(1):77–84.江世宏.深圳蝴蝶图鉴[M].广东：科学出版社,2019.PatelRK,JainM.NGSQCToolkit:Atoolkitforqualitycontrolofnextgenerationsequencingdata[J].PLoSONE,2012,7(2):e30619.JinJJ,YuWB,SongY,dePamphilisCW,YiTS,LiDZ.GetOrganelle:Afastandversatiletoolkitforaccuratedenovoassemblyoforganellegenomes[J].GenomeBilogy,2020,21(1):241.BerntM,DonathA,JühlingF,ExternbrinkF,FlorentzC,FritzschG,PützJ,MiddendorfM,StadlerP.MITOS:Improveddenovometazoanmitochondrialgenomeannotation[J].MolecularPhylogeneticsandEvolution,2013,69(2):313–319.MengG,LiY,YangC,LiuS.MitoZ:atoolkitforanimalmitochondrialgenomeassembly,annotationandvisualization[J].NucleicAcidsResearch,2019,47(11).ZhangD,GaoFL,JakovlićI,ZouH,etal.PhyloSuite:Anintegratedandscalabledesktopplatformforstreamlinedmolecularsequencedatamanagementandevolutionaryphylogeneticsstudies[J].MolecularEcologyResources,2019,20(1):348–355.PernaN,KocherT.Patternsofnucleotidecompositionatfourfolddegeneratesitesofanimalmitochondrialgenomes[J].JournalofMolecularEvolution,1995,41(3):353–358.Stamatakis,Alexandros.“RAxMLversion8:atoolforphylogeneticanalysisandpost-analysisoflargephylogenies.”

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