雨课堂学堂在线学堂云《Molecular Biology（中南）》单元测试考核答案

第1题ThegeneticmaterialofalllivingthingsisDNA.第2题Allgenesencodeproteinorpolypeptidechains第3题SomestructuralgenesonlyencodeRNA第4题Thepromoterisdirectional.第5题TheretroviralgenomeisRNA.第6题Structuralcharacteristicsofthehumangenome

ALargenumberofrepetitivesequencesBGeneoverlapCOperonstructureD

PolycistronicmRNA第7题Genomeis

AThesumofthegeneticmaterialofacompletesetofhaploidsinacellBAllDNAinthecellCAllRNAinthecellDAllchromosomesinthecell第8题Whichofthefollowingisacis-actingelement

ApromoterB

intronC

exonD

operon第9题

ViralgenomestructurecharacteristicsAgeneoverlapBSomeviralgenomesareRNAC

SomearesegmentgenomeD

Largenumberofrepetitivesequences正确答案：ABC第10题Bacteriagenomestructurecharacteristics

AOperonstructureB

IsogeneCAnoriginofreplicationDgeneoverlap正确答案：ABC第1题MismatchrepaironlyrepairsthemismatchedbaseincorporatedduringDNAreplicationineukaryoticcells.第2题SpontaneoushydrolysisofbasescancausepurinesorpyrimidinestoshedfromtheDNAchainribosephosphatebackbone.第3题Inadditiontoeasilycausingtheformationofpyrimidinedimers,ultravioletradiationcanalsocausedamagesuchasDNAstrandbreaks.第4题Cross-linkinginDNAdamageonlyreferstocross-linkingbetweenDNAmolecules.第5题DNAdamagecausedbyexogenouschemicalsismainlyrepairedbymismatchrepair.第6题UltravioletradiationcausesdimersbetweenthebasesofDNAmolecules,themostcommonformisAT-TBC-CCC-T

DT-U第7题IntheprocessofE.colimismatchrepair,themismatchednucleotidesareidentifiedbyAmutSBRecJCmutLD

mutH

第8题Whenadenineexistsintheformofiminotautomer,itcanbepairedwith

ACytosineBThymineCGuanineDHypoxanthine第9题IntheprocessofE.colinucleotideexcisionrepair,theroleofUvrBis

AIdentifythedamagesiteB

Unwindthedoublestrand

CCutson3ˊsideDCutson5ˊside正确答案：BC第10题DNAdamagecausedbyalkylatingagent

ABasealkylation

BBasesheddingC

DNAstrandbreakD

Pyrimidinedimer正确答案：ABCUnitassignmentforweek4第1题TheyieldofPCRproductsisonlyrelatedtothenumberofinitialtemplates.第2题Inthereal-timequantitativePCRreaction,theCtvalueofeachtemplatehasalinearrelationshipwiththelogarithmoftheinitialcopynumberofthetemplate.Thehighertheinitialcopynumber,thesmallertheCtvalue.第3题ThesecondaryantibodyusedinWesternblottingistoprotecttheprimaryantibody,therebyimprovingthesensitivityoftheprimaryantibody.第4题cDNAlibraryreferstoallcDNAclonesinaspecificcellandinaspecificstate.

第5题VectorisaDNAmoleculethatcancarrytheforeignDNAsegmentsandmakeitreplicateorexpressinthehostcell.

第6题

PlasmidsusuallyA

existwidelyinavarietyofmicroorganisms,includingbacteriaandyeastBcan'tbeinheritedinastablewayoutsidethechromosomesofhostcells.C

areextrachromosomalsingle-strandedcircularDNAmoleculesofthebacterialcell.Darevectorsforgenecloning,andtheymayholdforeignDNAfragmentsmorethan10kb.第7题NorthernblothybridizationisthatAItcanidentifythecontentandsizeofspecificmRNA.B

Itcandigestthenucleicacidtobetestedwithrestrictionendonucleases.CItcantransfertheprobetoasolidsupport.DItisaDNAanalysistechnology.第8题WhichisthecorrectdescriptionofSangersequencing?

A

ddNTPsubstrateterminatetheextension.BUsinghightemperaturetoterminatetheextensionreaction.C

ItisbasedonRNAstrandsynthesisreaction.DThereactionsubstrateisdNTPorNTP.第9题Reversetranscriptase

Ahas5ˊ→3ˊpolymeraseactivityBhas3ˊ→5ˊexonucleaseactivityCisanRNA-dependentDNApolymeraseDisaDNA-dependentDNApolymerase正确答案：AC第10题Processofgenecloningincludes

A

preparationoftargetgeneandappropriatevectorBinsertingtargetgeneintovector

C

introductionofrecombinantDNAintohostcellsDscreeningforandidentifyingrecombinantDNA正确答案：ABCD第11题

WhichofthefollowingiscorrectaboutPCR?

A

Theamplifiedfragmentisbeyondapairofprimers.BItisakindofinvitroamplificationtechnologyofDNAfragments.CTheproducthasbeenaccumulatingexponentially.DItincludesthreebasicprocesses,namelydenaturation,annealingandextension.正确答案：BCD第12题

Whichofthefollowingiscorrectaboutthegenechip?AItcansimultaneouslyanalyzethevariationofmultiplegenes.BMicroarraysampledensityisverysmall.C

Itcanbeusedtostudytheexpressionofmultiplegenes.DItisaregularlyarrangedcDNAoroligonucleotidearray.正确答案：ACDUnitassignmentforweek5第1题SDSisanelectrophoretictechniquetoseparateproteinsaccordingtotheirpolarity.第2题Proteinchipcanbeusedforantigen-antibodyscreening.第3题TheresultsofproteinseparationinSDSandnon-denaturingelectrophoresisarethesame.第4题Isoelectricfocusingisatechniquetoseparateproteinsaccordingtotheirmass-to-chargeratio.第5题Theyeasttwo-hybridsystemtostudyproteininteractionsrequirestheseparationoftargetproteins.第6题TheincorrectdescriptionabouttheprincipleofSDSis

A

SDScanseparateproteinswithdifferentisoelectricpoints.BSDSisananionicdetergent.CSDScanbecombinedwiththehydrophobicpartofprotein,sothattheproteincanobtainnegativecharge.DThemobilityofproteinsinSDSarerelatedtotheirmolecularsizes.第7题Theexperimentaltechniqueusedtostudyproteininteractionsis

Ayeasttwo-hybridizationB

InsituhybridizationCSAGE

Ddotblothybridization第8题Asameansofhigh-throughputproteinanalysis,themainadvantagesof2-DEliesin

ATheresultsof2-DEcontainingthousandsofproteinspotsharbouringdifferentisoelectricpointand/ormolecularsize.Bcontinuousandautomatedsamplepreparationandtwo-dimensionalgelelectrophoresis.Citscapabilityindetectingparticularlylowprotein.Ditscapabilityindetectinghydrophobicproteins.第9题Post-translationalmodificationsofproteinsincludeAglycosylationBmethylationCphosphorylationDacetylation正确答案：ABCD第10题Themainresearchmethodsofproteininteractionbasedonmassspectrometryinclude

AProteinchipBImmunocoprecipitationcoupledmassspectrometryCAffinitychromatographycoupledmassspectrometryDBiosensorcoupledmassspectrometry正确答案：ABCDUnitassignmentforweek6第1题Nonsensemutationistheproductionofmeaninglessproteinaftermutation.

第2题Missensemutationscansignificantlyaffectproteinfunctionandphenotype,leadingtodisease.第3题Restrictionendonucleaseisabase-specificendonuclease.

第4题Whichofthefollowingmethodsisnotincludedingenediagnosis?AGenetargeting

BPCR

CNucleicacidhybridizationDGenechip

第5题Whichiscorrectaboutindirectgenediagnosis

ADetectionoflinkagegeneticmarkersBDetectionofgeneexpressionproductsCBetterthandirectdiagnosisDThepathogenicgenemustbeknown第6题ThefollowingstatementaboutgenerecombinationisincorrectAGenerecombinationoccursonlyduringsexualreproductionBGenerecombinationcanproducemultiplegenotypesCNonallelesonnonhomologouschromosomescanundergorecombinationDGenerecombinationisthefundamentalsourceofbiologicalvariation第7题Thenecessarycasefordirectgenediagnosisis

AThenormalmolecularstructureofthetestedgenehasbeenidentifiedBThereisadirectcausalrelationshipbetweengenemutationsandthediseaseCThemutationsiteofthetestedgeneisknownDThereisnoothergenemutationsinthetestedsample正确答案：ABC第8题Genemutationismainlyachangeofdnaprimarystructure,whichcanbeachangeofsinglenucleotide,orachangeofmultiplenucleotidesorevenasequenceofnucleotides;itsmolecularmechanismcanbereplacement,insertionordeletion,etc.,thusproducingdifferentmutationtypessuchas

APointmutationBDeletionmutationCInsertionmutationDReverseormigrateinversion正确答案：ABDC第9题ThetargetmoleculeofgendiagnosisisjustDNA.

第10题Southernblottingcanbeusedforthediagnosisofgenedeletionmutations.Unitassignmentforweek7第1题RibozymeisaproteinmoleculethatcanshearsmallmoleculeRNAs.第2题Adenovirusvectorsarecapableofsiteintegration.

第3题Geneinterventioncanincreasetheexpressionofagenetotreatdisease.第4题Distanttumorcellscannotbeaffectedbysuicidegenebystandereffects.第5题Thereisnopromotersequencesinretroviralpre-virusLTR.第6题Whatisthecorrectdescriptionaboutthesuicidegenetherapystrategy？AThesuicidegeneshouldhavepost-introductionexpressionintherecipientcells.BDirectlyinh