雨课堂学堂在线学堂云《Molecular Medicine Techniques（锦州医科）》单元测试考核答案

第1题IntheprocessofgenomicDNAextraction,thepossibleoperationsthatcausethedegradationandfragmentationofmacromolecularDNAareAEquipmentsterilizationtreatmentBGentleoperationCAddEDTADRepeatedlypipettingtheDNApellettopromotedissolution第2题TheroleofEDTAinthecelllysisbufferusedtoextractgenomicDNAfromtissuesorcellsisADestroycellmembraneandnuclearmembraneBSeparatetissueproteinfromDNACInhibittheactivityofDNaseDDegradationofprotein第3题TheroleofproteinaseKusedtoextractgenomicDNAfromtissuesorcellsisADestroycellmembraneandnuclearmembraneBSeparatetissueproteinfromDNACInhibittheactivityofDNaseDDegradationofprotein第4题TheroleofRNaseAusedtoextractgenomicDNAfromtissuesorcellsisADestroycellmembraneandnuclearmembraneBSeparatetissueproteinfromDNACInhibittheactivityofRNaseDDegradationofprotein第5题TheratioofpureDNAOD260/OD280isaboutA1.8B1.6C1.5D2第6题AfterDNAextraction,iftheratioofOD260/CD280is2.0,thepossiblereasonisASaltorsmallmoleculepollutionBRNAcontaminationCProteincontaminationDOrganicsolventcontamination第7题AfterDNAextraction,iftheratioofOD260/CD280is1.5,thepossiblereasonisADNAdegradationBRNAcontaminationCProteincontaminationDSaltpollution第1题TheplasmidusedinDNArecombinationtechologyis：APartofthecellchromosomalDNABtheindependenthereditaryunitoutsideofthebecterialgenomicDNACpartoftheviralgenomicDNADpartoftheeukaryoticchromosomegenomicDNA第2题restrictionendonucleaserecognizethespecialstructureofDNA,thespecialstructureiscalled()ApalindromicstructureBmotifCsupersecondarystructureDtertiarystructure第3题TheenzymecanrecognizespecialsequencesandcleaveDNAatthesespecificbasesequencesiscalled?ADnaseBRestrictionextronucleaseCRestrictionendonucleaseDnon-Restrictionendonuclease第4题WhatarethemaincomponentofLysisBuffer?Asodiumhydroxide(NaOH)andSDS.BglucoseandTris-HClCEDTAandRNaseDAcetateandPotassiumacetate第5题WhichkindofelectrophoresisisfrequentlyusedtoanalyzeplasmidDNAasapowerfulseparationmethod?ASDSBAgrosegelelectrophoresisCPCRDWestern-BlotPrinciple,extractionoperationandidentificationofpolymerasechainreaction第1题PCRcomponentsinclude()AprimersBtemplateCTaqpolymeraseDNTP正确答案：ABC第2题PCRprocessincludesthreesteps,theyare()AdenaturationBannealingCextentionDcooling正确答案：ABC第3题Simplesetofrulesforprimersequencedesigninclude()APCRprimersshouldbe15-30nucleotidesinlength.BoptimalGCcontentoftheprimeris40%-60%.CpreferoneortwoGorCatthe3’-endofprimer.Davoidprimerselfcomplementarities.正确答案：ABCD第4题WhataretherequirementsforDNAtemplateinPCR?()ApurifiedDNAtemplate.Buse1pg-1ngofplasmidorviraltemplate.Cuse1ng-1μgofgenomictemplates.DUsethehigherDNAconcentrationswhenfewercycledesired.正确答案：ABCD第5题WhenweamplifyatargetDNAsequencebyPCR,howtodetermineannealingtemperature?()ATmBTm-5℃C55℃D60℃Principle,operationandcalculationofLowrymethodforproteindetermination第1题Thetotalproteinconcentrationisexhibitedbyacolorchangeofthesamplesolutioninproportiontoproteinconcentration,whichcanthenbemeasuredusing().AbalanceBspectrophotometerCcentrifugeDpipette第2题TheLowrymethodisbasedonthereactionof（）producedbytheoxidationofpeptidebondswithFolinreagentunderalkalineconditions.ACu2+BFe3+CMg2+DCa2+第3题ThereactionsinLowrymethodresultinastrongbluecolor,whichdependsmainlyontheaminoacidsresidues,suchas()and(),theintensityofwhichisrelatedtothecontentofprotein.AAlaGlyBArgAlaCTrpTyrDGluGly第4题InLowrymethod,thesamplesandthestandardproteinsolutionaretreatedidentically.Aftercolordevelopment,theabsorbanceofeachsampleisdeterminedbyabsorbanceat()nm,thustheproteincontentcanbecalculated.A550B710C280D650第5题ThecompositionofFolinreagentisamixtureof().AphosphomolybdateandphosphotungstateBHClandphosphomolybdateCphosphotungstateandH3PO4DphosphomolybdateandH3PO4Principle,operationandresultdisposalofSDS第1题WhichisrightfortheSDSPAGE?AintroductionofSDSsysteminpolyacrylamidegelBthemigrationdistanceisrelatewithproteinmolecularandchargeCtheconcentratedgelbufferispH10.0DtheseparationgelbufferispH10.0第2题Whenthemolecularweightfrom15kDto200kD,whathavelinearrelationshipwiththelogarithmofthemigrationrate?AmolecularweightofproteinBtheshapeofproteinCthechargeofproteinDthepIofprotein第3题Insaturatedstate,howmanySDSbindwithpergramofpeptide?Aabout10.4gramBabout0.4gramCabout1.4gramDabout0.14gram第4题InSDSPAGE,thegelbufferusedis?ATris-GlycineBTris-HClCNa2CO3-NaHCO3K2HPO3-KH2PO3第5题whatisthefunctionofbetamercaptoethanolwhenyoudealwiththesample?AbreakthehydrogenbondBbreakthe-S-S-bondCbreakthepeptidebondDbreakthephosphatebondPrinciple,operationandresultofAffinitychromatography第1题ProteinsseparationcanbecarriedoutonthebasisofAnetchargeBsolubilityinsaltsolutionsCsizeormassDallofthese第2题AffinitychromatographydealswiththeAspecificbindingofaproteinconstituentsforanothermoleculeBprotein-proteininteractionCprotein-carbohydrateinteractionDcarbohydrate-carbohydrateinteraction第3题Therearemanytypesofchromatography.ThereareAaffinitychromatographyBgelfiltrationchromatographyCion-exchangechromatographyDallofthese正确答案：第4题TheadvantageofaffinitychromatographyisAmoreefficientBverycheapCcanseparateallmacromolecularDcanseparateallmacromolecular第5题Theappropriateligandisattachedtothematrixofthecolumnandaproteinextractisrunthroughthecolumn.Theonlymacromoleculethatwillattachtothecolumnwillbetheonethatbindstotheligand;alltheothermacromoleculeswillwashthroughthecolumn.Thecolumnisthentreatedinsuchamannerthattheligandandthemacromoleculewilldissociateandthemacromoleculewilltheneluteoff.Elutionbyinclusionoffreeligandinbufferiscommon.OthermethodsofelutioncanbeAloweringthepHofbufferBincreasingordecreasingsaltconcentrationsCdecreasingthetemperatureofthecolumnDallofthese正确答案：Principle,operationandcalculationofKmvalueofalkalinephosphatase第1题WhichoneistheMichaelis-Mentenequation?()ABCD第2题TheMichaelis-Mentenequationdemonstratesthatwhenv=½Vmax,whichoneisthesame