ASK1-IN-12-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEASK1-IN-12Cat.No.:HY-179517分⼦式:C₂₄H₂₂F₃N₉O分⼦量:509.49作⽤靶点:ASK1;JNK;p38MAPK作⽤通路:Apoptosis;MAPK/ERKPathway储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性ASK1-IN-12⼀个ASK1抑制剂，其IC50为6.3nM。ASK1-IN-12可抑制TNF-α诱导的ASK1-p38/JNK通路激活。ASK1-IN-12能够减少游离脂酸诱导的胆醇升⾼、脂滴积累，并改肝细胞脂变性。ASK1-IN-12可⽤于⾮精性脂性肝炎(NASH)的研究[1]。体外研究ASK1-IN-12(Compound20)(1-6μM;24h)reducestotalcholesterol,triglyceride,low-densitylipoproteincholesterolandlipiddropletaccumulationandimproveshepatocellularsteatosisinfreefattyacid-inducedLO2cells[1].ASK1-IN-12(1-1000nM;24h)inhibitsTNF-α-inducedactivationoftheASK1-p38/JNKpathwayinHepG2cells[1].ASK1-IN-12(0.5-5μM;24h)exhibitsnosignificantcytotoxicitytoLO2hepatocytes,withcellviabilitymaintainedabove80%[1].WesternBlotAnalysis[1]CellLine:HepG2cellsConcentration:1,10,100and1000nMIncubationTime:24hResult:Reducdthephosphorylationratiosofp-ASK1/ASK1,p-JNK/JNK,andp-p38/p38.REFERENCES1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].LiuH,etal.PyrazolopyridinederivativesaspotentASK1inhibitors:design,synthesis,andbiologicalevaluation.BioorgChem.2025;167:109176.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792ax:0