虫媒病毒防控新策略诊断与疫苗研究进展

123401https:///initiatives/global-arbovirus-initiative#:~:text=Arthropod%2Dborne%20viruses%20(arboviruses),building%20a%20coalition%20of%20partners.RVFVWNVHTRVPOWVVEEVEEEVSFTSVCHIKV,

DENV,

ZIKV,

YFVZIKV2016CHIKV20144DENV•ZIKV

CHIKV

DENVhttps:///en/arbo-portal;

DOI:

10.1093/ofid/ofaf395https:///sections/goats-and-soda/2025/08/08/g-s1-81670/china-chikungunya-virus-mosquitoes,

Visual

China

Group/via

Getty

Images2025

78000CHIKV86.8%81.4%65.5%ECSA•1)2)3)3)•CHIKVDOI:

10.3390/vaccines9010032

02DOI:

10.1186/s12985-025-02622-zvs.vs.IL-8NeSt1IL-1β1.2./CCL2/7/12CXCL2/1.2.DOI:

10.1016/j.immuni.2016.06.002DOI:

10.1007/s00253-020-10437-xCHIKV02-74-8/••/

/••IgM•IgM-IgG+IgM03DOI:

10.1016/j.tim.2019.11.005IgMIgG/26-1422-3IgM6321IgM12-19IgG

IgMRT-PCR--(POC)FDADOI:

10.1016/j.tim.2019.11.005/DENVNS1,

IgM,IgGRDT/LFA,

ELISA,

IIFTSe:

95.9%

(95%

C.I.

86.0–99.5%);

Sp:

100.0%

(95%

C.I.

90.3–100%)ZIKVNS1,

IgM,IgGRDT/LFA,

ELISA,

IIFTSe:

69%

(59–79)

Sp:

96%

(92–100)CHIKVIgM,

IgG

E1/E2*IIFTSe:

95.7–98.5%;Sp:

93.4–99.6%TRFIAB2

B3

CHIKV

E2CHIKV-E2

(Cat#:

40440-V08B)ZIKV-E

(Cat#:

40543-V08B4)DENV-E

(Cat#:

40471-V08B)DOI:

10.1186/s12985-023-02132-wMetforminAMPKBlock

viralreplicativecomplexWNV,

DENV,ZIKV,

YFVCP640186Acetyl-CoAcarboxylaseBlocks

fatty

acid

synthesis

early

inthe

viral

life

cycleDENV,

ZIKV,WNVSunitinib/ErlotinibAAK1/GAKBlocking

intracellular

trafficking

byAAK1-GAKDENVBenzenesulfonamideCaMKIIDecrease

viremia

level

and

increasesurvival

time

in

a

mouse

modelDENV,

ZIKVBerberineMAPKHindering

nucleocapsid

assemblyCHIKVApoptozoleHSP70block

viral

replicationDENV,

ZIKVAXL

AAK1

GAK5---DOI:

10.1186/s12985-025-02622-z--TemoporfinNS2B–NS3

proteaseChemicalZIKV,

DENV-2NitazoxanideNS2B–NS3

proteaseChemicalZIKV,

DENV-2,

JEV(1–6,

1–3)-β-D-glucanNS1ChemicalZIK,

DENVSofosbuvirNS5-RdRPChemicalYFV,

DENV,

ZIKVGNF-2

and

2-12-2E

ProteinPROTACDENV-2,

ZIKA,

JEV,WNV,

YFVML336nsP2ChemicalVEEV,

EEVDOI:

10.1186/s12985-025-02622-z;

DOI:

10.1038/s41467-024-49161-9••Capsid

Membrane

EnvelopeNS1

NS2A

NS2B

NS3

NS4A

NS4B

NS5MG-132:

Proteasome

inhibitorMLN4924:

Neddylation

inhibitorLenalidomide:

CRBN

ligandGNF-2:

Binding

ligand

to

E2-12-2:

Binding

ligand

to

EGNF-2-deg-BUMP:

Negative

Control2-12-2-deg-BUMP:

Negative

ControlGNF-2:

Binding

ligand

to

E2-12-2:

Binding

ligand

to

EGNF-2-deg-BUMP:

Negative

Control2-12-2-deg-BUMP:

Negative

ControlDENV

EGNF-2-deg/2-12-2-deg

CRBNDOI:

10.1038/s41467-024-49161-9GNF-2:

Binding

ligand

to

E2-12-2:

Binding

ligand

to

EGNF-2-deg/2-12-2-degDOI:

10.1038/s41467-024-49161-9/10.3390/biologics4010001DENVDengvaxiaETAK-003-IIIZIKVZKADNA085-00-VP-DNAIIMV-ZikaprM/sEICHIKVVIMKUNYAE1/E2/CapsidCHIKV

VLPIXCHIQ-WNVWN-VAX-IIChimeriVax-WN02PrM/EYF17DIIJEVIXIARO-YFVYF-VAXEILV/CHIKVIgM

IgG181/25

CHIKV/IRESB

CD8+CHIKVTDOI:

10.1080/22221751.2021.1886598EILV/CHIKVTDOI:

10.1038/s41541-024-01047-z04DENV-EDENV-NS1DENV-CDENV-NS5A

CHIKV-E2ZIKV-EZIKV-NS1ZIKV-MZIKV-NS5YFV-EYFV-PrMWNV-EWNV-NS1SFTSV-GcSFTSV-GnSFTSV-NPRVFV-GRVFV-LHTRV-GcHTRV-GnPolyprotein

CHIKV

(UVE/CHIKV/2024/RE/CNR_79903)

E2

Protein

(His

&

AVI

Tag)

|

Cat#:

41094-V49HPurity:

≥

90%

as

determined

by

SDS-PAGE

&

SEC-HPLC.Loaded

CHIKVE2Antibody(Cat#:

40440-MM20)on

AMC

Biosensor,can

bind

CHIKV(UVE/CHIKV/2024/RE/CNR_79903)

E2

Protein

(Cat#:

41094-V49H)

with

an

affinity

constant

of

6.51

nM

asdetermined

in

BLI

assay

(Sartorius

OctetRED384)

(Routinely

tested).Immobilized

CHIKV

(UVE/CHIKV/2024/RE/CNR_79903)

E2Protein

(Cat#:

41094-V49H)

at1

μg/mL

(100

μL/well)

canbind

rabbit

anti-

CHIKV

antibody,

the

EC50

is1.6

ng/mL(Routinely

tested).CHIKV41094-V49HCHIKV-E2UVE/CHIKV/2024/RE/CNR_79903HEK293

Cells≥

90%His

&

AVI41094-V49H-BCHIKV-E2UVE/CHIKV/2024/RE/CNR_79903HEK293

Cells≥

90%His

&

AVI41094-V08HCHIKV-E2UVE/CHIKV/2024/RE/CNR_79903HEK293

Cells≥

90%His40440-V08BCHIKV-E2strain

SL-CK1Baculovirus-InsectCells>

90%His40440-VCCBCHIKV-E2strain

SL-CK1Baculovirus-InsectCells≥

95%No

tag

DENVDENV-C40262-V07EE.

coliHis>

90%Strain

New

Guinea

CDENV-E40532-V08H1HEK293

CellsHis>

95%type3,

strain

Philippines/H87/1956DENV-E40471-V08BBaculovirus-InsectCellsHis>

80%type2,

strain

New

Guinea

C/PUO-218

hybridDENV-E40531-V08BBaculovirus-Insect

CellsHis>

95%type

1,

strain

US/Hawaii/1944DENV-NS140528-V07HHEK293

CellsHis>

90%strain

Philippines/H87/1956DENV-NS140263-V07HHEK293

CellsHis>

90%Strain

New

Guinea

CDENV-NS5A40267-V07E1E.

coliHis>

95%Strain

New

Guinea

CWNVWNV-E40345-V08YYeastHis>

95%Lineage

1,

strain

NY99,

Isolate

385-99WNV-E40345-V08Y1YeastHis>

95%Lineage

1,

strain

NY99,

Isolate

385-99WNV-E40534-V08Y1YeastHis>

95%lineage

2,

strain

Nea

Santa-Greece-2010WNV-NS140346-V07HHEK293

CellsHis>

95%Lineage

1,

strain

NY99,

Isolate

385-99ZIKVZIKV-E40543-V08B4Baculovirus-InsectCellsHis>

90%ZikaSPH2015ZIKV-E40543-V08HHEK293

CellsHis>

90%ZikaSPH2015ZIKV-E40543-V08H-BHEK293

CellsHis>

90%strain

Zika

SPH2015ZIKV-M40543-V02H2HEK293

CellshFc>

95%ZikaSPH2015ZIKV-NS140544-V07HHEK293

CellsHis>

95%ZikaSPH2015ZIKV-NS540546-V08BBaculovirus-InsectCellsHis>

85%ZikaSPH2015POWVPOWV-Polyprotein41072-V08EE.

coliHis≥

90%RVFVRVFV-G40338-V07B1Baculovirus-InsectCellsHis>

90%Strain

MP12RVFV-G40338-V08BBaculovirus-InsectCellsHis>

85%Strain

MP12RVFV-L40423-V08EE.

coliHis≥

90%strain

TAN/Dod-002/07SFTSVSFTSV-Gc41067-V08H1HEK293

CellsHis≥

90%Human/China/HB29/2010SFTSV-Gn41067-V08HHEK293

CellsHis≥

90%Human/China/HB29/2010YFVYFV-E41052-V49B-BBaculovirus-InsectCellsHis

&

AVI≥

90%strain

17D

vaccineYFV-E41052-V08BBaculovirus-InsectCellsHis≥

90%strain

17D

vaccineYFV-PrM41052-V53EE.

coliHis

&

GST≥

85%strain

17D

vaccine

Chikungunya

virus

(CHIKV)

E2Chikungunya

virus

(CHIKV)

E240440-MM2540440-MM26Detectsample:

CHIKV(UVE/CHIKV/2024/RE/CNR_79903)

E2

protein

(Cat#:41094-V49H)ELISAWBCat#:

40440-MM26Cat#:

40440-MM32The

CHIKV

E2

antibodies

(Cat#:40440-MM20,

40440-MM02,40440-MM33,

40440-MM26,

40440-T46)

can

bind

to

thecoated

CHIKV

(UVE/CHIKV/2024/RE/CNR_79903)

E2

protein(Cat#:

41094-V49H)

(0.02

μg/ml)

with

highaffinity

by

ELISAassay.Sample:

CHIKV

(UVE/CHIKV/2024/RE/CNR_79903)E2

Protein

(Cat#:

41094-V49H)

(10

ng)

IgZIKVZIKV-E40543-R101WBRabbit

MAbRabbit

IgGZIKV-E40543-MM35ELISA

(Cap)MouseMAbMouseIgG1ZIKV-E40543-R029WBRabbit

MAbRabbit

IgGZIKV-E40543-R033ELISA

(Det)Rabbit

MAbRabbit

IgGZIKV-NS140544-MM03ELISA

(Cap)MouseMAbMouseIgG1ZIKV-NS140544-MM10ELISA

(Det)MouseMAbMouseIgG1ZIKV-NS140544-MM15WB,

ELISAMouseMAbMouseIgG1DENVDENV-C40262-MM05WB,

ELISAMouseMAbMouseIgG2aDENV-C40262-T46WBRabbit

PAbRabbit

IgGDENV-E40471-M144WB,

ELISAMouseMAbMouseIgG1DENV-E40471-M201WBMouseMAbMouseIgG1DENV-NS140263-T62WB,

ELISARabbit

PAbRabbit

IgGDENV-NS140263-MM02ELISAMouseMAbMouseIgG1DENV-NS140263-MM12ELISAMouseMAbMouseIgG1DENV-NS5A40267-MM02WB,

ELISAMouseMAbMouseIgG2bDENV-NS5A40267-T62WB,

ELISARabbit

PAbRabbit

IgGDENV-NS5A40267-RP01ELISARabbit

PAbRabbit

IgGCHIKVCHIKV-E240440-MM15ELISAMouse

MAbMouseIgG1CHIKV-E240440-MM25ELISA,

ELISA

(Cap)Mouse

MAbMouseIgG1CHIKV-E240440-MM26WB,

ELISA,

ELISA

(Det)Mouse

MAbMouseIgG2bCHIKV-E240440-MM32WB,