DiR-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDiR(solution)Cat.No.:HY-DY1013CASNo.:100068-60-8分⼦式:C₆₃H₁₀₁IN₂分⼦量:1013.42作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性DiR(solution)⼀种长链碳菁染料。碳菁染料⼴泛⽤作亲脂性⽰踪剂，⽤于标记细胞、细胞器、脂质体、病毒和脂蛋⽩。溶剂及浓度：DMSO：5mM体外研究GeneralProtocol1.PreparingStainSolutionsofDiRa.Prepareworkingsolutions:Dilutethestocksolutionsintoasuitablebuffersuchasserum-freeculturemedium,HBSSorPBStomake1to5μMworkingsolutions.Wedonotrecommendstoringtheaqueoussolutionformorethanoneday.2.Suspensioncellsa.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofDiRworkingsolution,andthenincubateatroomtemperaturefor5-30minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.3.Adherentcellsa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30minutes.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEd.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.REFERENCES[1].GanWB,etal.Multicolor"DiOlistic"labelingofthenervoussystemusinglipophilicdyecombinations.Neuron.2000Aug;27(2):219-25.[2].SwiftMJ,etal.AppliedelectricfieldsacceleratethediffusionrateandincreasethediffusiondistanceofDiIinfixedtissue.JNeurosciMethods.2005Jan30;141(1):155-63.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-