M464-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEM464Cat.No.:HY-179494分⼦式:C₂₃H₂₈O₄S₃分⼦量:464.66作⽤靶点:NOD-likeReceptor(NLR);Pyroptosis;Caspase;InterleukinRelated;ReactiveOxygenSpecies(ROS)作⽤通路:Immunology/Inflammation;Apoptosis;MetabolicEnzyme/Protease;NF-κB储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性M464⼀种⾮甾体类抗炎化合物，⼀种强效且⼝服有效的NLRP3炎症⼩体抑制剂。M464通过抑制ASC寡聚化和减少ROS的产⽣，抑制细胞焦亡(pyroptosis)，并阻断下游Caspase-1的表达和IL-1β的释放。M464对⼩⿏急性肺损伤和肝损伤具有保护作⽤。M464可⽤于NLRP3相关炎症性疾病的研究[1]。IC50&TargetNLRP3inflammasome体外研究M464(3-12μM)inhibitstheexpressionofmarkerproteinsassociatedwithNLRP3inflammasomeactivation(IL-1βandCaspase-1),andrestrainstheproductionofintracellularROSandtheformationofASColigomersinJ774A.1andTHP-1cells[1].M464(3-12μM;1h)inhibitspyroptosisinmacrophagestriggeredbytheNLRP3inflammasomestimulatorinJ774A.1andTHP-1cells[1].体内研究M464(15,30,60mg/kg;i.g.;singledose;1hourbeforeLPSinjection)mitigatesLPS(HY-D1056)-inducedacutelunginjuryinmicebyinhibitingNLRP3inflammasomeactivation[1].\nM464(15,30,60mg/kg;i.g.;singledose;1hourbeforeLPSinjection)mitigatesLPSandD-GalN-inducedacuteliverinjuryinmicebyinhibitingNLRP3inflammasomeactivation[1].AnimalModel:MaleKMmice(42-56daysold)intraperitoneallyinjectedwithLPS(10mg/kg)[1]Dosage:15,30,60mg/kgAdministration:i.g.;singledose;1hourbeforeLPSinjection1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEResult:Dose-dependentlyreducedthepathologicalscoresoflunginjury.Significantlyimprovedthelungtissuedamageinmice.Inhibitedthelungwet/dry(W/D)weightratioandsignificantlyimprovedpulmonaryedemainmice.SignificantlyreducedthelevelsofmatureIL-1βandCaspase-1.AnimalModel:MaleKMmice(42-56daysold)intraperitoneallyinjectedwithLPS(50μg/kg)andD-GalN(800mg/kg)[1]Dosage:15,30,60mg/kgAdministration:i.g.;1hourbeforeLPSinjectionResult:ReversedtheLPSandD-GalNinducedincreaseoftheliver-to-bodyweightratio.MarkedlyattenuatedLPS/D-GalN-inducedliverinjuryinmice.Exhibiteddose-dependentsuppressionofserumlevelsofASTandALT.EffectivelysuppressedthematureIL-1βreleaseandCaspase-1productioninadose-dependentmanner.EffectivelyreversedtheLPS/D-GalN-inducedreductioninSODcontentandincreaseinMDAlevel.REFERENCES[1].WangW,etal.M464inhibitsactivationofNLRP3inflammasomeandinflammatoryresponseinmice.SciRep.2025;15(1):33878.Published2025Sep30.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplicat