PKH-26-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPKH26(solution)Cat.No.:HY-DY1011CASNo.:154214-55-8分⼦式:C₅₉H₉₇IN₂分⼦量:961.34作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性PKH26(solution)⼀种红荧光染料，PKH26可以稳定的与细胞膜脂质区结合并发出红⾊荧光(Ex/Em=551/567nm)，主⽤于细胞体外标记、体外细胞增殖研究以及体内外的细胞⽰踪研究。溶剂及浓度：DMSO：5mM体外研究GeneralProtocol1.PreparationofPKH26workingsolution1.1PreparationofthestocksolutionDissolve1mgPKH26in1mLDMSOtoobtain1mMofstocksolution.Note:Itisrecommendedtostorethestocksolutionat-20°Cand-80°Cawayfromlightandavoidrepetitivefreeze-thawcycles.1.2PreparationofPKH26workingsolutionDilutethestocksolutioninserum-freecellculturemediumorPBSdiluteat1:100or1:200toobtain5-10μMofworkingsolution.Note:PleaseadjusttheconcentrationofPKH26workingsolutionaccordingtotheactualsituation.2.Cellstaining2.1Suspensioncells(6-wellplate)a.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofworkingsolution,andthenincubateatroomtemperaturefor10-45minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyor1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEflowcytometry.2.2Adherentcellsa.Cultureadherentcellsonsterilecoverslips.b.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor5-30minutes.d.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.StorageStoreinseparatecontainerstoavoidrepeatedfreeze-thawcycles.-80°C,1yearProtectfromlightPrecautions1.PleaseadjusttheconcentrationofPKH26workingsolutionaccordingtotheactualsituation.2.ThisproductisforR&Duseonly,notfordrug,household,orotheruses.3.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].FischerK,etal.TheflowcytometricPKH-26assayforthedeterminationofT-cellmediatedcytotoxicactivity.Methods.2003;31(2):135-142.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.For