miRNA functional similarity and functional pdf课件

1、2010 Bioinformatics IF:4.877,Inferring the human microRNA functional similarity and functional network based on microRNA-associated diseases,整体思路,miRNA,disease,通过两个疾病集合的相似性衡量两个miRNA的相似性,基本假设：相似的miRNA通常和相似的疾病相关联,两个疾病集合的相似性,假设已知两个疾病的相似性,则一个 疾病和一个疾病集合的相似性定义为,两个疾病集合的相似性定义为,也就是两个miRNA的相似性,两个疾病集合的相似性,Cont

2、ents,Abstract,Motivation 对于gene功能相似性和功能网络的研究是很普遍的，也是有意义的，由于miRNA功能注释的有限性，对miRNA 功能相似性的研究很少 Results,本文的贡献,Introduction,研究领域,前人的工作,问题所在,Introduction,Gene功能相似性： 序列 表达 功能(注释到GO),本文的 工作,序列和表达相似性并不总是和功能相似性相关的 靶基因有较高的假阳性和假阴性率,Methods,MeSH disease DAG structure Human miRNA-disease association data Method fo

3、r measuring miRNA functional similarity Semantic value of a disease Semantic similarity of two diseases miRNA MISIM functional similarity Construction of miRNA functional network,miRNA功能相似性衡量方法,miRNA-Disease,Methods,MeSH Category C,miRNA:271个 Disease:137个,Disease DAG,Sementic value of a disease,疾病 t

4、 在疾病A的 DAGA中对疾病 A 的语义值的贡献称为 t 的 D 值，记为 DA(t),Semantic value of a disease,语义贡献因子 本文取0.5,Semantic similarity of two diseases,一个疾病和一个疾病集合的相似性,两个miRNA的功能相似性,Results,MISIM functional similarity of miRNAs miRNA MISIM functional similarity is correlated with expression similarity miRNAs in the same family

5、or cluster show high MISIM functional similarity Novel highly functionally similar miRNAs MISIM functional similarity of two miRNAs is correlated with the fraction of their common targets A miRNA functional network Comparisons with other semantic similarity methods,positive correlation,Results,expre

6、ssion similarity,same family or cluster,novel highly functional similar miRNAs,相似性分数比随机的高,positive correlation,Results,expression similarity,same family or cluster,novel highly functional similar miRNAs,相似性分数比随机的高,positive correlation,miRNA和其宿主基因倾向于共表达，看宿主基因的功能,Results,expression similarity,same fam

7、ily or cluster,novel highly functional similar miRNAs,逻辑关系 假设：若两个内含子miRNA的宿主基因功能相关， 则这两个miRNA功能相关. 分析：得分高的两个miRNA其宿主基因的功能若重 叠很多，则它们功能相关. 高分的miRNA倾向于功能相关,positive correlation,Results,fraction of their common targets,miRNA functional network,other semantic similarity methods,文中选取阈值 0.7,positive correlation,Results,fraction of their common targets,miRNA functional network,other semantic similarity methods,it seems that the cutoff 0.7 or 0.8 is suitable for the construction of miRNA functional networks.,文中选取阈值 0.7,positive correlation,不如本文的方法好,Results,fraction of their common targets,m