常用缓冲溶液的配制和PH计校正溶液配置方法

1、。一种普通缓冲溶液的制备方法1.甘氨酸-盐酸缓冲液(0.05毫升/升)x毫升0.2摩尔/升甘氨酸y毫升0.2摩尔/升盐酸，然后用水稀释至200毫升公共卫生XY公共卫生XY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸的分子量为75.07，0.2摩尔/升甘氨酸溶液的含量为15.01克/升.2.邻苯二甲酸-盐酸缓冲液(0.05摩尔/升)0.2摩尔/升邻苯二甲酸氢钾0.2摩尔/升盐酸，然后用水稀释至20毫升酸碱度(20)XY酸碱度(20)XY2.22.42.62.83.0555554.0703.9603

2、.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾的分子量为204.23，0.2摩尔/升邻苯二甲酸氢钾溶液含40.85克/升3.磷酸氢二钠-柠檬酸缓冲液公共卫生0.2摩尔/升Na2HPO4(毫升)0.1摩尔/升柠檬酸(毫升)公共卫生0.2摩尔/升Na2HPO4(毫升)0.1摩尔/升柠檬酸(毫升)2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8

3、216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4的分子量为14.98，0.2摩尔/升溶液的分子量为28.40克/升.Na2HPO42H2O的分子量为178

4、.05，0.2摩尔/升溶液含35.01克/升.C4H2O7H2O的分子量为210.14，0.1摩尔/升溶液的分子量为21.01克/升.4.柠檬酸-氢氧化钠-盐酸缓冲液公共卫生钠离子浓度(摩尔/升)柠檬酸(g)C6H8O7H2O氢氧化钠(g)氢氧化钠97%盐酸(毫升)盐酸(浓缩)最终体积(升)2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010(1)使用时，每升可加入1g苯酚。如果ph值最终发生变化，用少量50

5、%氢氧化钠溶液或浓盐酸进行调节，并将其储存在冰箱中。5.柠檬酸-柠檬酸钠缓冲液(0.1摩尔/升)公共卫生0.1摩尔/升柠檬酸(毫升)0.1摩尔/升柠檬酸钠(毫升)公共卫生0.1摩尔/升柠檬酸(毫升)0.1摩尔/升柠檬酸钠(毫升)3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.0

6、18.6柠檬酸C6H8O7H2O:分子量210.14，0.1摩尔/升溶液21.01克/升.柠檬酸钠Na3 C6H5O72H2O的分子量为294.12，0.1摩尔/升溶液为29.41克/毫升。6.乙酸-乙酸钠缓冲液(0.2摩尔/升)酸碱度(18)0.2摩尔/升NaAc(毫升)0.3摩尔/升荣誉炮兵连(毫升)酸碱度(18)0.2摩尔/升NaAc(毫升)0.3摩尔/升荣誉炮兵连(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404

7、.103.002.101.400.900.60Na2Ac3H2O的分子量为136.09，0.2摩尔/升溶液的分子量为27.22克/升.7.磷酸缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲溶液(0.2)公共卫生0.2摩尔/升Na2HPO4(毫升)0.2摩尔/升亚硫酸氢钠(毫升)公共卫生0.2摩尔/升Na2HPO4(毫升)0.2摩尔/升亚硫酸氢钠(毫升)5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.0

8、45.07.07.17.27.37.47.57.67.77.87.98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3Na2HPO42H2O的分子量为178.05，0.2摩尔/升溶液的分子量为85.61克/升.Na2HPO412H2O的分子量为358.22，0.2摩尔/升溶液的分子量为71.64克/升.Na2HPO42H2O的分子量为156.03，0.2摩尔/升溶液的分子量为31.21克/升.(2)磷酸氢二钠-磷酸二氢钾缓冲溶液(1/15摩尔/升)公共卫生摩尔/升/15

9、Na2HPO4(毫升)mol/L/15KH2PO4(毫升)公共卫生mol/L/15 Na2SO 4(毫升)mol/L/15KH2PO4(毫升)4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509:008.007.006.005.004.007.177.387.738.048.348.678.187.008.009:009.509.759.9010.003.002.001.000.500.250.100Na2HPO42H2O的分子量为178.05，1/15M溶液的分子量为11.876克/升.KH2PO4

10、的分子量为136.09，1/15M溶液的分子量为9.078克/升.8.磷酸二氢钾-氢氧化钠缓冲溶液(0.05米)x毫升0.2M K2PO4 Y毫升0.2N氢氧化钠，用水稀释至29毫升酸碱度(20)x(毫升)y(毫升)酸碱度(20)x(毫升)y(毫升)5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.6809.巴比妥钠-盐酸缓冲液(18)公共卫生0.04M巴比妥钠溶液0.2V盐酸公共卫生0.04米巴比妥钠溶液(毫升)0.2N盐酸(毫升)6

11、.87.07.27.47.67.88.08.210010010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥钠盐的分子量为206.18；0.04米溶液为8.25克/升10.tris盐酸缓冲液(0.05米，25)将50毫升0.1M Tris溶液与x毫升0.1N盐酸混合，然后用水稀释至100毫升。公共卫生x(毫升)公共卫生x(毫升)7.107.207.307.407.507.607.707.807

12、.908.0045.744.743.442.040.338.536.634.532.029.28.108.208.308.408.508.608.708.808.9026.222.919.917.214.712.410.38.57.0三羟甲基氨基甲烷(Tris)HOCH2 CH2OHCHOCH2 NH2分子量=121.14；0.1M溶液为12.114克/升.Tris溶液可以吸收空气中的二氧化碳，所以使用时要注意保持瓶盖紧闭。11.硼酸-硼砂缓冲溶液(0.2M硼酸盐)公共卫生0.05毫升硼砂0.2M硼砂(毫升)公共卫生0.05毫升硼砂0.2M硼酸(毫升)7.47.67.88.01.01.52.0

13、3.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O7H2O，分子量=381.43；0.05米溶液(=0.2M硼酸盐)含有19.07克/升.H2BO3硼酸，分子量=61.84，0.2M溶液12.37克/升.硼砂容易失去结晶水，必须储存在有塞子的瓶子里。11.硼酸-硼砂缓冲溶液(0.2M硼酸盐)公共卫生0.05毫升硼砂0.2M硼酸(毫升)公共卫生0.05毫升硼砂0.2M硼酸(毫升)7.47.67.88.01.01.52.03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O710H2O，分子量=381.43；0.05米溶液(=0.2M硼酸盐)含有19.07克/升.H2BO3硼酸，分子量=61.84，0.2M溶液12.37克/升.硼砂容易失去结晶水，必须储存在有塞子的瓶子里。12.甘氨酸-氢氧化钠缓冲液(0.05米)x毫升0.2M甘氨酸y毫升0.2M氢氧化钠用水稀释至200毫升公共卫生XY公共卫生XY8.68.89.09.29.450505050504.06.08.812.016.89.69.8