《肌肉系统解剖》PPT课件.ppt

1、Muscle Tissue 肌组织,Ying Ding Email:,The content,VCD: The function of skeletal muscle Observation: Skeletal muscle 骨骼肌 HE, NO.21 special stain shown LCT in yellow color 荧光桃红 NO.22 (2) Cardiac muscle 心肌 HE, NO.19 special stain shown intercalated disc 铁苏木素染色看闰盘 NO.20 Smooth muscle 平滑肌 HE, NO.23 Homework

2、: Draw the longitudinal and cross section of skeletal muscle fiber in high power objective lens 40, and describe its construction features. 作业：在高倍镜下描绘骨骼肌纤维的纵、横切面，并描述其结构特征。, 肌组织的分类、分布：,分类,分布,光镜,骨骼肌,心 肌,平滑肌,躯干、四肢,心 脏,内脏、血管壁,横纹肌,横纹肌,无横纹,收缩,随意肌 快 不持久,不随意肌 有节律 持久,不随意肌 慢 持久,(1)Skeletal muscle 骨骼肌 HE NO.21

3、,Naked eye: The section of tongue is pink color. 肉眼：舌头切面，染成粉红色 Lowest power objective lens 4: The most tissue is stained in pink. 低倍镜4*：表面凹凸不平，深蓝色的为舌黏膜上皮，深面为骨骼肌，大部分染成粉红色。,Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of skeletal muscle fi

4、bers. 低倍镜10*：纵、斜、横切面,Skeletal muscle fibers (lower magnification),Cross section,Longitudinal section,Blood vessel (oblique section),Lower power objective lens 10: This is longitudinal section of skeletal muscle fibers. Compare the staining of muscle tissue with LCT. What are their differences? 低倍镜10

5、*：骨骼肌的纵切面。 试比较其与疏松结缔组织的不同。,Skeletal muscle fibers (longitudinal section, lower magnification),(skeletal muscle fibers),loose connective tissue,High power objective lens 40: Analyses the structure in different sections of individual skeletal muscle fiber. Multi nuclei are peripheral location just und

6、erneath of the sarcolemma, which can be seen in various sections.,(the nucleus of skeletal muscle fiber),(skeletal muscle fiber),a skeletal muscle fiber(cross section),myofibrils(cross section),myofibrils(longitudinal section),loose connective tissue,高倍镜40*：分析不同切面的骨骼肌特征。,多核，位于肌纤维的周边，紧靠肌膜内面，不同切面均可见。,

7、High power objective lens 40: In the longitudinal of skeletal muscle fiber the myofibrils are very thin parallel with the length of the cell. And identify its cross alternative light and dark band (cross stria) with the diaphragm closed down. 40*镜纵切面：与长轴平行的细丝为肌原纤维。缩小光圈区分明暗相间的横纹-明暗带。,(the nucleus of

8、skeletal muscle fiber),(skeletal muscle fiber),(loose connective tissue),cross stria,High power objective lens 40: In the cross section of skeletal muscle fiber the myofibrils are stippled (dot) appearance. The delicate LCT surrounding each skeletal muscle fiber can be seen, which is termed endomysi

9、um. 40*横切面：肌原纤维呈细点状结构，周围包绕的细致疏松结缔组织为肌内膜。,a skeletal muscle fiber (cross section),myofibrils (cross section),myofibrils (longitudinal section),绘图格式,图名， 染色方法，放大倍数,图题,？,？,？,？,？,标出骨骼肌某些典型结构的名称,用简单的话描述其结构特征,Special stain: In order to easily define muscle tissue with CT, using this special stain to recogn

10、ize them. In this staining the muscle tissue is in red, CT is in yellow. 特殊染色（荧光桃红）： 肌组织红色，结缔组织黄色。,Special stain:,Skeletal muscle fibers (red) Loose connective tissue (yellow),(2) Cardiac muscle心肌 HE NO.19; special stain, NO.20,Naked eye: The section of the wall of heart is pink color. 肉眼：心脏切面染成粉红色。

11、 Lowest power objective lens 4: Move the slide you will find various sections of cardiac muscle fibers. 低倍镜4*：找到不同切面的心肌纤维。,通过比较观察心肌的形态结构（光镜）,肌纤维整体形态 肌浆（胞浆） 胞核 有无特殊结构 肌纤维间的结缔组织,Lower power objective lens 10: To identify various sections of the cardiac muscle fibers. Among the cardiac muscle fibers th

12、ere is less amount of LCT and rich capillaries. Look for the perfect cross and longitudinal section of cardiac muscle fibers. Then turn to Lens 40 . 10*不同切面。肌纤维之间有少量疏松结缔组织和毛细血管。找到典型的横、纵切面转到高倍镜下观察。,blood vessle,blood vessle,Loose connective tissue,cross section,Cardiac muscle (low magnification),long

13、itudinal section,High power objective lens 40: Compare to the skeletal muscle fibers. In the longitudinal of cardiac muscle fibers, which are branching and anastomosing with others, the cross-striated banding pattern is visible but not so clear than that of skeletal muscle fibers. The nucleus is loc

14、ated in the center, and surrounding by light cytoplasm due to without myofibrils. Try to find intercalated disc, which is darkly stained straight or step-like line crossing the fibers at interval.,Cardiac muscle fibers (longitudinal section-high magnification),intercalated disc,The nucleus of cardia

15、c muscle fiber,cross-striated band,blood vessle,40*纵切面：心肌纤维呈短柱状，有分叉，互相吻合。明暗相间的横纹不如骨骼肌明显。核呈卵圆形，位于细胞中央，两端无肌原纤维，被浅染的胞浆包绕。在心肌纤维连接处染色较深的结构为闰盘，呈直线或者梯形。,High power objective lens 40: In the cross section of cardiac muscle fibers, they are different size and appearance, the large and round appearance with o

16、r without centrally nucleus, the large and pear-like appearance with or without centrally nucleus, and the small and round shaped only with stippled appearance myofibrils.,loose connective tissue,blood vessel,a cardiac muscle fiber (cross section),40*横切面：肌纤维呈大小不一的块状。细胞核位于中央，核周围淡染。肌纤维切面有的比较大，圆形或梨形，伴或

17、不伴细胞核，有的比较小，呈圆形，仅为斑点状的肌原纤维。,Special stain: In section NO.20, the intercalated disc is shown clearly. 特殊染色能清楚地显示闰盘。,Intercalated disc,fibroblast,the nucleus of cardiac muscle fiber,(3) Smooth muscle平滑肌 HE NO.23,Naked eye: The section of the intestine is pink color. 肉眼：小肠切片，染成粉红色。 Lowest power objecti

18、ve lens 4: Look for the smooth muscle in pink color. 低倍镜4*：找到染成粉红色的平滑肌。,Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of smooth muscle fibers. 低倍镜10*：不同切面的平滑肌（纵横斜切面）。,Longitudinal section,loose connective tissue,oblique section,Smooth muscle (low magnification),High power objective lens 40: In longitudinal section, The smooth muscle cell is spindle-shaped with an elongated centrally located nucleus. The narrow part of one cell lied adjacent to the broad parts of