肝细胞癌中的表达南京医科大学.ppt

1、1,The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma,徐 智 南京医科大学附属南京第一医院暨 南京市第一医院 肿瘤内科,Worldwide incidence of HCC,Blum HE, Spangenberg HC. Arch Iran Med. 2007 Jul;10(3):361-71.,Identification of Yes-associated Protein (YAP),Zender, L. et al. Cell 125, 1253-67,E,F,G,H,YAP

2、improves cell proliferation,Time (Days),*：p0.05。,YAP induces anchorage-independent growth,MIHA-YAP cells but not MIHA-Vec could form colonies in soft-agar.,MIHA-YAP cells gained advantages in cell migration and invasion.,YAP enhances cellular mobility and invasiveness,IHC results of YAP in HCC,53.7%

3、 HCC samples showed nuclear YAP positive.,Clinical association of nuclear YAP in HCC,Clinical association of nuclear YAP and HCC-specific postsurgical recurrence,Kaplan-Meier,Median RFT： Nuclear YAP negative: 25.8 months vs. Nuclear YAP positive：12.9 months,Nuclear YAP is an independent prognostic m

4、arker for HCC recurrence,Cox Regression,Summary,YAP confers tumorigenic and metastatic potentials in hepatocytes, as evidenced by enhanced aptitudes in cell viability, anchorage-independent growth, migration and invasion. YAP nuclear accumulation was found in the 53.7% of HCC, correlated with poorer tumor differentiation and higher serum alpha-fetoprotein (AFP) levels. Nuclear YAP is an independent predictor for HCC-specific recurrence-free survival. YAP plays an important role in HCC development and progression.,Acknowledgement,新加坡国立大学外科系和药学系 Asso. Prof. John M. Luk 香港大学外科系 Pr