血必净注射液治疗火器致兔ali的康复价值及对肺组织nf―κb表达的影响_第1页
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-精选财经经济类资料- -最新财经经济资料-感谢阅读- 1 血必净注射液治疗火器致兔 ALI 的 康复价值及对肺组织 NFB 表达的 影响 摘要 目的 探 血必净 (XBJ)注射液治疗火器致兔急性肺损 伤(ALI)的康复价值及对肺组织核因 子-B(NF-B)表达的影响。 方法 采 用随机数字表法将 SPF 级健康雄性兔 60 只分为正常对照组、ALI 模型组、生 理盐水组、低剂量 XBJ 治疗组、中剂量 XBJ 治疗组、高剂量 XBJ 治疗组,每 组各 10 只。ALI 模型组、生理盐水组、 低剂量 XBJ 治疗组、中剂量 XBJ 治疗 组、高剂量 XBJ 治疗组吸入高温气体, 制作完成兔 ALI 动物模型;建模半小时 -精选财经经济类资料- -最新财经经济资料-感谢阅读- 2 后,低、中、高剂量 XBJ 治疗组分别腹 腔内注射 10、20、30 mg/kg XBJ,生理 盐水组注射生理盐水,正常对照组和 ALI 模型组不做处理。观察 12 h 后,处 死兔并取左肺上叶肺组织,检测肺组织 NF-B 的表达;在光镜下对肺组织进行 病理学检查,并及时记录其病理变化。 结果 病理学检查结果:正常对照组肺 泡结构清晰,肺泡壁薄,肺泡内未见水 肿液;ALI 模型组、生理盐水组肺泡结 构模糊,肺泡壁断裂,肺内大量炎症细 胞浸润,肺组织间质及肺泡腔出现明显 水肿,可见较多的红细胞,肺泡壁间质 增厚,透明膜形成;经 XBJ 治疗的各组 肺泡结构仍存在,肺内炎症细胞浸润减 轻,肺组织间质及肺泡腔中度水肿,可 见少量渗液、出血。肺组织 Western blot 分析显示,ALI 模型组 NF-B 活性 明显高于正常对照组(P 中国论文网 /6/view-12954805.htm 关键词 急性肺损伤;血必净注 射液;核因子-B;细胞因子 -精选财经经济类资料- -最新财经经济资料-感谢阅读- 3 中图分类号 R563 文献标识码 A 文章编号 1673-7210(2017) 11(a)-0019-05 The value of Xuebijing Injection on the rehabilitation of rabbits with acute lung injury induced by firearm injury and the effect on the expression of NF-B in lung tissue XU Xiaofeng XU Xiujuan HAO Jian WU Sheng QIAN Jun LI Shuwen LI Xiaoqian Cardiopulmonary Rehabilitation Medicine Center, Hangzhou Sanatorium of Peoples Liberation Army, Zhejiang Province, Hangzhou 310007, China Abstract Objective To investigate the value of Xuebijing Injection (XBJ ) on the rehabilitation of rabbits with acute lung injury (ALI ) induced by firearm injury and the effect on the expression of nuclear factor kappa B (NF-B ) in lung tissue. Methods Sixty healthy male -精选财经经济类资料- -最新财经经济资料-感谢阅读- 4 rabbits were randomly divided into normal control group, ALI model group, normal saline group, low dose of XBJ treatment group, medium dose of XBJ treatment group, high dose of XBJ treatment group by random number table mehtod, with 10 rats in each group. The rabbits of ALI model group, normal saline group, low dose of XBJ treatment group, medium dose of XBJ treatment group, high dose of XBJ treatment group were inhaled with high-temperature gas, so as to complete the model of rabbits with ALI. After modeling for half an hour, low, medium, high dose of XBJ treatment groups were respectively received intraperitoneal injection of 10, 20, 30 mg/kg XBJ, normal saline group was injected with normal saline, while normal control group and ALI model group were not dealt with any treatment. After 12 h, the rabbits were -精选财经经济类资料- -最新财经经济资料-感谢阅读- 5 killed and the lung tissue of left upper lobe was taken to detect the expression of NF- B in lung tissue; under the light microscope, the lung tissue was taken to make pathological examination, and the pathological changes were recorded in time. Results The results of pathological examination: in the normal control group, the alveolar structure was clear, the alveolar wall was thin, and no edematous fluid was found in the lung tissue; in the ALI model group and normal saline group, the alveolar structure was fuzzy, the alveolar wall was broken, there were a lot of inflammatory cells infiltration in the lung, obvious edema was found in the pulmonary mesenchyme and alveolar space, many red blood cells could be seen, and the mesenchyme of alveolar wall was thickened, the hyaline membrane was formed; after treatment -精选财经经济类资料- -最新财经经济资料-感谢阅读- 6 by XBJ, the alveolar structures in all groups were still existed, the inflammatory cells infiltration in the lung was reduced, pulmonary mesenchyme and alveolar space showed moderate edema, less cataclysm and bleeding could be seen. Western blot of lung tissue showed that, the activity of NF-B in ALI model group was significantly higher than that of normal control group (P 0.05) 。从病理上观察,ALI 模型组、生 理盐水组、低剂量 XBJ 治疗组、高剂量 XBJ 治疗组兔肺组织与正常对照组比较, 其肺泡壁、肺泡腔、肺间隙、肺毛细血 管均发生明显的

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