生物分离工程第章一绪论.ppt

生物工程下游技术 （Downstream technology of bioengineering ） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 学时安排 （40学时） n课堂授课 35 学时 n实验演示 3 学时（实验室） n讨论与交流 1 学时 n期末复习 1 学时 n答疑 2 学时（非课堂） n自学 4 学时（非课堂） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 学时安排 目录学时目录学时 第一章 绪论3第六章 吸附分离技术 4 第二章 细胞破碎 3 第七章 色谱技术 5 第三章 生物大分子的 初级分离技术 3第八章 电泳技术 4 第四章 膜分离技术 5第九章 蛋白质的复性 2 第五章 萃取技术 4第十章 干燥、结晶 2 理论结合实验演示3讨论与交流、复习总结2 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 参考书目 n参考教材： 生物分离工程 孙彦编著，化学工业出版社，2005年 。 n主要参考书目 生物工程下游技术 刘国诠主编，2002年。 生物分离工程田瑞华，科学出版社，2008年 生物产品分离纯化技术 李从军等，华中师范大学 出版社，2009年。 Bioseparation Process ScienceAntonio A. Garca等。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 第一章 绪论 3学时 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 内容提要 n生物下游加工技术简介 n生物下游加工过程的特点 n分离机理和分离操作 n生物物质 n分离效率的评价 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 学习目的和要求 n要求：对本课程有比较初步的认识； n掌握：生物下游加工技术的特点；生物分离过程 的单元操作原理； n了解：生物分离技术的研究内容和发展情况；生 物物质的种类和特性；下游加工过程的一般流程 ； n应用：运用生物分离技术的评价方法分析实际问 题。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物工程研究领域 n微生物培养 n动物细胞培养 n植物细胞培养 n天然资源 n生化材料 n海洋生物培养 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物工程产业领域 n生物制药（抗生素，基因重组蛋白，氨基酸，疫苗，菌苗，天 然药物，生化药物，血液制品，抗体，多糖，多肽） n生物化工（乳酸，柠檬酸，苹果酸，丙烯酸，甘油，异丙醇， 乙烯） n生物能源（甲醇，乙醇，生物柴油，生物汽油） n生物材料（明胶，胶原蛋白，人造皮肤，从造骨，人造脏器） n生物医学 （诊断试剂，基因治疗，人及生物克隆） n环境生物（环境治理，水污染，土壤污染，风沙治理） n生物食品（醋，啤酒业，酿酒，乳制品，奶制品） n生物资源（动物，植物，微生物） n生物农业 （基因食品，基因植物） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物工程的上中下游 n上游：菌种，基因工程，分子生物学，遗传 学 n中游：微生物发酵工程，动植物细胞培养 海洋生物培养 n下游：生物分离工程 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 菌种筛选 摇瓶实验 发酵罐实验 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n生物技术的目标就是指利用培养微生物 、动物细胞、植物细胞来生产对人有用 的产品。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 何谓工程（学）？ The application of scientific and mathematical principles to practical ends such as the design, manufacture, and operation of efficient and economical structures, machines, processes, and systems. 将科学及数学原理运用于实际用途的应用手段， 如设计、制造并操纵高效、经济的结构、机器、过 程及系统 -美国传统词典 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 早期的生物工程的萌芽 美国和英国合作对青霉素进行生产研究 （1941年），出现表面培养：1升扁瓶 或锥形瓶，内装200mL麦麸培养基 40u/ml Alexander Fleming （1928） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物工程的萌芽 19431943年沉浸培养：年沉浸培养： 5m 5m 3 3 200u/ml 200u/ml 当今：当今：100m100m 3 3 200m200m 3 3 5-7 5-7万万u/mlu/ml Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离工程的萌芽 Golden Time概念的提出 血液制品的生产 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n1953年，DNA双螺旋结构的发现 n1972年，美国斯坦福大学构建了第一个重 组DNA分子 n1996年，克隆羊“多利”诞生在英国的罗斯 林研究所 n1990-2006，人类基因组计划 n现在，生物信息、生物芯片、胚胎干细胞等 生命科学的发展带动生物工程的进步 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 现代生物技术的兴起 n两项技术 第一个含有外源基因的重组质粒在细菌内增殖 成功（1973年） 细胞融合技术（1975年） n直接结果： 上述两项技术的直接此前天然存在于生物体内 极微量的生物物质得以通过大量生物培养方式 进行商业的生产，出现了现代生物加工过程。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物加工过程内容 n优育生物物种的选育； n生物反应生产粗原料； n目标产物的分离纯化过程； 基因工程 遗传与育种 生物反应工程 生物分离工程 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 下游加工过程的沿革 n传统产业（第一代） n19世纪60年代-20世纪50年代 酒精，丙酮，丁醇 n第二代生物技术产品 20世纪40年代 n抗生素，有机酸，核酸，酶制剂，单细胞蛋白 n第三代 20世纪70年代中期 n动物细胞培养 n植物细胞培养 n基因工程发酵产品 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物下游加工过程的一般步聚 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 结构基因组的研究内容 Bottleneck Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 结构基因组研究的制约因素 在蛋白质克隆和表 达系统被不断优化的 同时，分离纯化过程 蛋白质的错误折叠及 产物的不溶及聚合已 成为结构基因组计划 发展的制约因素。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离技术与化工分离技术的区别 n化工分离技术：获得纯的化学物质 n生物分离技术：在得到纯的生物物质同时，还必 须关注特定杂质的去除。 与传统的化学试剂的纯 度概念不同，生物产物对 有害物质有严格的控制， 生产过程也要求有严格的 管理，在最终产品中往往 不允许有极微量的有害杂 质存在。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 工业应用的生物分离技术 n 回收技术: 絮凝，离心，过滤，微过滤。 n 细胞破碎技术: 球磨，高压匀浆，化学破 n 碎技术 n 初步纯化技术: 盐析法，有机溶剂沉淀， n 化学沉淀，大孔吸附树剂，膜分离技术 n 高度纯化技术: 各类层析，亲和，疏水， n 聚焦，离子交换 n 成品加工： 喷雾干燥，气流干燥，沸腾干 n 燥，冷冻干燥，结晶 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离与一般化学分离方法的比较 n大约80%的化工分离方法可应用于生物分 离技术； n生物分离一般比化工分离难度大； n成分复杂：固体成分包括完整有机体、 培养基及底物中的不溶物。液体成分包括 底物可溶物、代谢中产物及目标产物。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n悬液中的目标产物浓度低：获得高纯度 的干燥产品、需要几步、分离过程精细 ，成本高。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物技术产品的类型 n按分子量大小 n小分子产品：（小于1000）抗生素，有机酸，氨基 酸 n大分子产品：（大于1000）酶, 抗体，多肽，蛋白质 n按产品所处的位置 n细胞内: 胰岛素，干扰素，重组蛋白质 n细胞外：抗生素，胞外酶 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 发酵液中主要的产品含量 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n生物产品要求高质量： n*purity（纯度） n*sanitation（卫生） n*biological activity（生物活性） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n分离过程的成本占产品总投资的大部分 ：因此必须仔细考虑和设计产品的回收 和纯化过程。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 设计中应考虑下列问题： n1. What is the value of the product? （产品价值） n2. What is an acceptable product quality? (产品质量） n3. Where is the product in each process stream? （产物在生产过程中出现的位置） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. n4. Where are the impurities in each process stream?（杂质在生产过程中 出现的位置） n5. What are the unusual physicochemical properties of the product and the principal impurities? （主要杂质独特的物化性质是什么？） n6. What are the economics of various alternative separations? （不同分离方法的技术经济比较） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离技术的重要性 n生物产物的特殊性； n生物产物所处环境的复杂性； n对生物产品要求的严格性； 最终结果：导致下游加工过程度成本往往 占整个生物加工过程生产成本的大部分。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 下游加工技术的一般流程 生物下游加工过程是指目 标产物的分离纯化过程， 包括 产物提取(isolation) 产物浓缩(concentration) 产物纯化(purification) 成品化(polishing) 注意： 多步分离导致收率降低； Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Separation Processes: Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Extractors 溶质在二个互不相容的溶剂的分配 系数不同，所造成的溶质的转移。 混合器和澄清器 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离一般分四步 nA：不溶物的去除 n过滤 n离心 n细胞破碎 n产物浓度和质量得到了提高 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. B:产物分离 n离子交换吸附 n萃取 n a. 溶剂萃取 n b. 反微团萃取 n c. 超临界流体萃取 n d. 双水相萃取 n 以上分离过程不具备特异性，只是进行初分,可提 高产物浓度和质量 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. C:产品纯化 n色谱 n电泳 n沉淀 n以上技术具有产物的高选择性和杂质的去除性 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. nD. Polishing （产品的精制） n *crystallization（结晶） n *drying （干燥） Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 中药动态水提取生产线流程图 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. * Ethanol from fermentation. 蒸馏器冷凝器 冷凝器 酒精 可溶性产品 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. * Citric acid manufacture 菌丝体 柠檬酸 石灰膏剂 废 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. * Penicillin production. 反萃取 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Production of bakers yeast面包酵母 糖蜜 发酵 麦芽汁 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Production of monosodium glutamate 味精 注射器 结晶 油水分离器 母液 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of an extracellular enzyme Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of intracellular enzymes Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of penicillin 霉菌 菌丝体 杂质 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of single-cell protein on a methanol basis甲醇底物 甲醇 絮凝 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of toxoid vaccines类毒素疫苗 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Recovery of whole-cell vaccines 澄清器Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 分离技术的选择依据 n产物所处的位置； n产物性质（分子大小、疏水性 、电荷形式和溶解度等）； n生物加工过程自身的规模和产 品的商业价值；一种目标产物 的分离手段往往不止一种，根 据生产的规模和价值，选择合 适的分离技术 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物下游加工过程的特点 n满足维持生物物质活性的要求 n满足快速分离的要求 n满足纯度和杂质去除的要求 n满足高效分离的要求 n满足成本优化的要求 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile .Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 生物分离本质 有效地识别混合物中不同溶质间物 理、化学和生物学性质的差别，利用能 够识别这些差别的分离介质和（或）扩 大这些差别的分离设备实现溶质间的分 离或目标组分的纯化。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client