根神经节来源许旺细胞及联合聚乳酸及壳聚糖神经导管修复坐骨神经缺损.doc

SICENCE新闻发布 作者：蒋华军 稿号：NRR-205-13根神经节来源许旺细胞及联合聚乳酸及壳聚糖神经导管修复坐骨神经缺损现有研究中分离许旺细胞容易受到成纤维细胞的污染，且成本高、效率低。来自中国大连医科大学第一附属医院蒋华军博士所带领的团队收集新生小鼠的脊髓背根神经节，以无血清的黑素细胞培养基纯化许旺细胞，其获得的许旺细胞纯度可达95%以上，远高于含胎牛血清的DMEM培养基提取的许旺细胞。这些许旺细胞复合聚乳酸-羟基乙酸共聚物/壳聚糖导管修复大鼠10mm坐骨神经缺损，发现大鼠坐骨神经组织轴突直径及轴突面积显著增加，运动功能明显改善。表明无血清的黑素细胞培养基更容易纯化许旺细胞，且许旺细胞复合聚乳酸-羟基乙酸共聚物/壳聚糖神经导管可有效的应用于修复缺损的坐骨神经。相关文献发表于中国神经再生研究（英文版）杂志2014年11月第22期。取新生小鼠的背根神经节，酶消化分散后以黑素细胞培养基进行纯化许旺细胞后，许旺细胞的增殖速度较快，而成纤维细胞生长受到抑制Article: Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats, by Li Zhao1, Wei Qu2, Yuxuan Wu2, Hao Ma2, Huajun Jiang2 (1 Department of Anesthesiology, Heji Hospital Affliated to Changzhi Medical College, Changzhi, Shanxi Province, China; 2 Department of Orthopedics, First Affliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China)Zhao L, Qu W, Wu YX, Ma H, Jiang HJ. Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats. Neural Regen Res. 2014;9(22):1961-1967.欲获更多资讯： Neural Regen Res DRG-derived SCs combined with PLGA/chitosan conduits for repair of sciatic nerve defects Isolation and purification of Schwann cells (SCs) is complicated by contamination with fibroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. Huajun Jiang, at First Affiliated Hospital of Dalian Medical University, China and his colleagues collected dorsal root ganglia from neonatal rats and obtained highly purified SCs using serum-free melanocyte culture medium. The purity of SCs ( 95%) was higher than that of cells purified using standard medium containing fetal bovine serum. The obtained SCs were implanted into poly(lactic-co-glycolic acid)(PLGA)/chitosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were significantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental findings suggest that serum-free melanocyte culture medium is conducive to purify SCs and PLGA/chitosan nerve conduits combined with SCs contribute to restore sciatic nerve defects. These results were published in Neural Regeneration Research (Vol. 9, No. 22, 2014).Dorsal root ganglia from neonatal rats were digested with enzyme and the obtained Schwann cells were purified using serum-free melanocyte culture medium. The purified Schwann cells proliferated rapidly and the growth of fibroblast was inhibited. Article: Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats, by Li Zhao1, Wei Qu2, Yuxuan Wu2, Hao Ma2, Huajun Jiang2 (1 Department of Anesthesiology, Heji Hospital Affliated to Changzhi Medical College, Changzhi, Shanxi Province, China; 2 Department of Orthopedics, First Affliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China)Zhao L, Qu W, Wu YX, Ma H, Jiang HJ. Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats. Neural Regen Res. 2