MICROELECTRODE TECHNIQUES FOR CELL PHYSIOLOGY：细胞生理微电极技术.doc

MICROELECTRODE TECHNIQUES FOR CELL PHYSIOLOGY26th Workshop, 9th 23rd September 2009The Marine Biological Association of the UK,Citadel Hill, Plymouth, PL1 2PB.Information for ApplicantsThe workshop provides intensive practical experience of a number of microelectrode, patch clamp and optical techniques applied to single cells. It is intended for postgraduate students, post-doctoral workers or established scientists wishing to apply these techniques in their research. The following core techniques are offered as 3-day experiments: Two electrode voltage clamp Patch clamp single channel, whole cell, slice recording Single electrode voltage-clamp Dye injection Ion-selective microelectrodes Fluorescent indicators In addition there are lectures and practicals in:Electronics, microscopy, data analysis and computing, bilayer recording, flash photolysis, amperometry and capacitance measurements, multi-electrode arrays, interface chamber blind recording, in vivo patch clamp recording. There are 20 places. Daily lectures are given by teachers and visiting lecturers to cover the basic techniques and specialised topics. A copy of the Plymouth Microelectrode Handbook will be provided. Accommodation (for 14 nights- arrive & depart on Wednesday) is close to the laboratory and includes breakfast, lunch is provided in the lab each day and an allowance is given for an evening meal. The course fee of 1200 includes accommodation, meals and tuition. Participants are responsible for their own travel arrangements. The closing date for applications is 30th April 2009 How to ApplyThere is no application form. A printed application sent by post AND emailed word / pdf file are required. 1. Please give a concise description of your research, your reasons for wishing to attend and your experience of techniques taught on the workshop. List in order of priority four 3-day techniques that you would like to learn selected from the list. 2. Provide a brief CV (2 sides), including list of publications. 3. The application must be accompanied by a letter of recommendation from an academic referee, preferably PhD supervisor or Head of Laboratory. This letter should indicate how your research, the laboratory in which you work and the area of research that you intend to pursue will benefit from your participation in the workshop. 4. What is your likely source of funding? FundingUK Research CouncilsAt present there is no guaranteed fee agreed for Research Council students for 2009. Application should be made with MRC, BBSRC or EPSRC through appropriate channels for support from the studentship.Affiliates of the Physiological Society Help with funding is usually available for young physiologists working in the UK. If you wish to apply please indicate in your application to the workshop. There is no need to apply directly to the Physiological Society before workshop applications are assessed. We will make a block application after. Information is on /grants/BursariesThe workshop can provide half bursaries - if you think you will have difficulty finding the full feeand will need a half bursary, please indicate in your application. Applications should be sent to:Mrs Alexandra StreetMicroelectrode Techniques, Marine Biological Association of the UK, Citadel Hill, Plymouth PL1 2PB UKE-mail microelectrodemba.ac.uk Information on internet (this page): http:/www.mba.ac.uk/education.php Application deadline 30th April 2009Applications will be acknowledged on receipt. A meeting to assess applications will occur during May and all applicants will be notified of the outcome. 2008 TIMETABLE- 2009 will be very similar!MICROELECTRODE TECHNIQUES FOR CELL PHYSIOLOGY25th Workshop 10-24 September 2008LECTURE TIMETABLE Wednesday 5ish Registration 6.00 Film:- The Squid and its giant nerve fibre Thursday9.00 Introduction David Ogden11.00 Electronics - Chris Courtice, Andy Hill, Bill Potter, Narinder Gill, Martyn Stopps, Voi Piotrowski, Ted Dyett, Derek LewisFriday9.00Voltage Clamp Techniques Ken Wann11.30 Patch Clamp David Ogden 2.00 Single Electrode Voltage ClampAlasdair Gibb4.00 Ion-selective microelectrodesJuha Voipio5.00 Permeabilised patch recordingKarri Lms6-00 Conventional and Confocal Microscopy Brad AmosSaturday9.00 Fluorescent Indicators Martin Thomas 10.30 Microscopy Demonstrations Brad AmosExperiments Block 1 Saturday Tuesday 6.00 Dye injection techniques Dave Becker Monday 9.00 Self referencing microelectrodesPhil Langton Demonstration Multi electrode recording in cardiac muscleThomas Meyer6.00Computers and Data Analysis John DempsterTuesday9.00 Capacitance measurements Gregor Zupani6.00 Slice recording and cell stimulationBoris BarbourWednesday9.00 Recent developments in patch clamp Hubert AffolterExperiments Block 2 Wednesday - Friday6.00 In Vivo recording from the mammalian brainTroy MargrieThursday 9.00 Extracellular field potentials and LTPTim Bliss 6.00 Analysis of single channel recordsDavid Colquhoun Friday9.00 Interpretation of single channel data David Colquhoun Demonstration Multi electrode recording in brain slicesTommas Ellender6.00Multi electrode arrays - recording network activity. Ole Paulsen Saturday9.00 Solutions-basic properties and ion replacementsDavid OgdenExperiments Block 3 Saturday - TuesdayMonday9.00 Flash Photolysis David OgdenDemonstr