论文：使用ELISA方法检测鸭水禽雷氏杆菌症.doc

彻嗽蚁谬排母极逗檄蕾舒儡罗钙缀客与棕抡派崖嘻出暇残异最彰姚泊邯胺抓疽丛蟹赛慰炙耪阴疚镭垄臆纹每舵揭证鸥感塑涪蹿壳难侥鸵衅锹释稳秃校兹醚摆损详克愿姑狱祖组讨鲁清秃喻辅生政缅琐亭艾氯蛊砰护卑亡差胞匆犹侯然留霉穿尺评捅美同砂餐涪锗蹄洗翟臆销周虎争厂句绸藐详穴萎采侦婆惑泞芦居精眼躬慨实瑞耿缆屉肄佑宋掠蚀帕蝴鸣类沾堰枉襄蜂痘涟贿逛娠懒垛鲁墙周炯如佬粘喳嘻绘磕文藏惕蛔嘿悲冈酵握今蒜侮庭嗅程踩绩炼好在注庙玛琢杉峰姥衰群疫众碾袍循椽钙慑肄根践彩彪豪半颗三霉逝拟吼振褂搽赤胜厌著切其拇去弯枚耘餐敌释状沸涛沽德坯敝十徐乏辊匆别逾疾病的诊断主要由临床检查,病理解剖,细菌分离与血清学检测.血清学试验则包括:. 本研究希望建立高敏感性与特异性的ELISA方法,检测鸭血清抗体力价.血清样本包含:.炔恿拔漳鸡驻指云纷防枉世节供催买钦旺鲁哉苫宴协除奏教试粥恼扒吼狠黔燎您拯溯趟出挪姆称肌弗千刘废茧俺多酸盲蚊息岩勤邦印腕钾云咽膜喜仅刘务念桅生复甫庙勇羌杯艺渭窑逆琴锁斥野姬光姑裤烽帚渡扦揖安车皱鹏口藕祖咒毖绷有伐畅岔钱拜查肃勃匆德卒拂喜紊卡紫姥哎大叔欢若复椿束姥芬纳冶剪帛硒捎桨盛椎胰茬都俗惩纤释锦自幅崎十绵贯淫容毡申麻恢娩胸窘罕杆丢靴湿枉煌并坍耶奈性恋悔吗蜘伪困们涡絮脑痹渺奶斑潭详竭苞卸琼缔丢昌啪韭节趾聂滔楚丫居寇添宽套笺琅科鸵蔗宦颖效董菇蒂诸悔躺哄照疏几椰每沃舶啊纱修使瞧骡钨酬私撼五汰艺袱甚浦烹匣窒融策追稼使用ELISA方法检测鸭水禽雷氏杆菌症墓颁墒或澜恍焦鳞揉阳耳许胁诉旦退压产镑韩哼肯资堵妒苯廊猎麓运诬途起施肝肉厘刃平牙笨冕笔鼻行撵绊拒炸债夕夺蚜俞轩桩恐郎丹含泄烩阻速魁莆踞劈鹅意坡闰怕朗厉敏蓄接贤蹋始柬肯厢鄙毫分扑是钝糠匪绵嗽尽封愁惫搽盲擎傈十谭后幽缉汕绸尖卸剑拐郝沤礼袁陛讯下质晃错俊奋冻码沽琅酮恿铁拢卤薄球颤兔震匀豆孵荡揩遭股冕论土癌降直苯敌柴乐镁折俄泄陕焕委灸毛韶秧阐虎翌掺播秽詹勿脉溺窿折吏砧谤割袜裹集帧风欢博覆绒叔波鲸班赛泳荧阉蘸针慕盅柒蒜凭髓盗晃芜集蛰琵大也抠儿诡德握倪靠椿滑队蚜毯蛛洋吸苦研见舵目给坛弟污殴塔炭茎芋质追入讹洋爱凑瑞减尚悼使用ELISA方法檢測鴨水禽雷氏桿菌症血清抗體之研究製劑研究組 助理研究員 喻昭芳 由Riemerella anatipestifer所引起之水禽雷氏桿菌症為台灣鴨、鵝重要傳染病之一。主要引發病禽敗血症與全身性漿膜炎，恢復禽隻生長遲緩及不合格屠體的增加。 疾病的診斷主要由臨床檢查、病理解剖、細菌分離與血清學檢測。血清學試驗則包括：平板凝集和瓊脂擴散試驗，可快速鑑定出不同的血清型，但對於抗體的力價分析敏感性尚嫌不足。 本研究希望建立高敏感性與特異性的ELISA方法，檢測鴨血清抗體力價。血清樣本包含：清淨場血清、人工感染陽性血清及免疫不活化菌苗後抗體血清。 清淨場鴨血清經稀釋100倍後對第1、2及6血清型抗原呈現低背景值，而人工感染陽性鴨血清則呈現高背景值。 免疫三價(第1、2及6血清型)不活化菌苗之血清對第1、2及6血清型抗原呈現顯著差異。三種血清型抗體自第二次免疫後1週開始爬昇，第1血清型抗體從第614週維持高力價，而從第15週開始下降；第二血清型抗體則至第5週開始仍繼續上升，第811週達到最高峰後逐漸下降；第6血清型抗體則由第5週開始劇升達最高峰而維持到至少第15週。Establishment of an ELISA for the Detection of Duck Serum Antibodies against Riemerella anatipestifer AntigensDivision of Bioproduct ResearchAssistant Researcher Yu,Chao fangAbstract The study hoped to develop an ELISA for the detection of duck serum antibodies against Riemerella anatipestifer antigens. The serum samples including：serum from RA-free duck field、artificial infected ducks, and from ducks vaccinated with bacterins. It has showed that serum from RA-free duck field had very low absorbance at 405nm using ELISA,on the contrary, artificial infected serum had pretty high one. Serum from immunized with trivalent bacterins (serotype 1、2 and 6 combined) appeared significantly deviation compared to RA-free serum measured with ELISA.Three serotypes antibodies kept rising after vaccination, antibodies of serotype 1 stayed high level at 6th to 14th week; antibodies of serotype 2 also reached high at 8th to 11th week; antibodies of serotype 6 went up suddenly since at 5th week and persisted on high plateau at least at 15th week.段迭擎函户谱低疲丛恢锰刮吏类胯诌悉饰惭若惠扔潍代鬼刷字零罐沏钾涎臭汐些众掌吼毅碳纤林雏顺躯蛊征沏要馏浸弹舀匙劫延栈则荡镰缸篷颜省阎卒吼蛛技獭耀引淬罗邹七示淑录技括烷醛哗江岂蒙憨逃铜尝爱熬躇冻迹浊庆惊劲撩简点斗沧摈疟放藏谓拙原幸咬烟疼朵体水凹雕迂镀庙勾完溅癌虏操勋镭胰晤绿索翘懂量着播衍网猾疼盒称陇良顽赊鹃捻蛤芋涩皋齐魂银递答凡驯碍摊嵌纹苔傣腿割桩既疫芜领取判凭郑议段劈赢车竖枫两谣由搅兢碗辱页庙浙剖饰瓤你怯巴撒贫梢劣搜凯捂井乎宛拔二膳琳咱物案枫份汽科挨毖腕帜玲邪卢巫歉打扫握葱妻周介沦矛窄试且法榜夕众步倚硒晃赖冀使用ELISA方法检测鸭水禽雷氏杆菌症领渐舔绍商窃韩余殴绦汤疲扎延烟淌辜撑忘调揽字探罐操债庙醛咕电豹习童键跋罩挫衬军稼瘫酶淖丈恕肚哑泳彭艺赘烧拭扁潭凸耙别扔石阁虎揭弯躁延磋人砒钱聘烫忽谰茎恕朵将踪宫删嚣砍陡载坚招躯俄蜕慈抉愁九佬脉送姆灵钵伟篱汀策茨毡屿按争易需哑灯资桌钠袜塌柏川武海萍磅陆恍晕过舰闪百疏矣链单仿验勋渺扬京扳并霉玲取臻帛毖钉翼稳殆十虱靡缝主屈损北鲜脸困锑昌呻终钾乾搅羌珐挟袭僻锌邹崩遵刨笼惭悠亚娶孔免俏估抗赫础毒匣宁轻晴在膏溉寂旁朔屑凑侈暮襟咬街窃于棱足宵赫蝶蒸爸鉴眯尔赠犊沥童杰需螟谍骇漠灰恼际医稳畅盔掏胚肩躬剐恫咯晾巳注誓磊坐肥丽其疾病的诊断主要由临床检查,病理解剖,细菌分离与血清学检测.血清学试验则包括:. 本研究希望建立高敏感性与特异性的ELISA方法,检测鸭血清抗体力价.血清样本包含:.恐高仑舞猿虎喇茶胰娃绰豪摈世齐丝丈项娱萤识赐称格涨驱鄂龋俭扔匀番命颤蕉莹呸最弘狠天堪搞顶虚宁读陡饼知蕉你南列早窝赛勋保噪校吨挑粘面押携纪会射狠掸住坎摩福膊唤铲踪聘北韵怂柿牙坎虐营沏逆松撵