Bcl┐2及Bag┐1基因产物在肾癌组织中的表达及意义.doc

Bcl2Bag1 :Bcl-2;Bag-1; :Bcl-2Bag-1.SABC4110.Bcl-275.6%30.0%P<0.05P>0.05.Bag-161.0%10.0%.Bag-1P<0.05.Bcl-2Bag-1r=0.438.Bag-1;Bcl-2. Keywords:genesBcl-2;Bag-1;kidneyneoplasms;immuno-histochemistry;geneexpression Abstract:AIMToinvestigateBcl-2andBag-1expressionsinrenalcellcarcinomasandtoevaluatetheirclinicalsignificance.METHODSBcl-2andBag-1expressionswerestud-iedwithimmunohistochemstryStreptavidin-Biotin-PeroxidaseComplexSABCmethodsin41samplesofrenalcarcinomatissuesandin10normalrenaltissuesandtheirclinicalsig-nificancewereanalysed.RESULTSThepositiverateofBcl-2expressionintherenalcarcinomatissueswas75.6%whileitwas30.0%inthenormalrenaltissuesP<0.05.Ac-cordinglythepositiverateofBag-1expressioninthecarci-nomatissueswas61.0%itwas10.0%inthenormaltissuesP<0.05.HoweverBcl-2wasnotrelatedtothetumorgradeclinicalstageandpathologicalcelltypes.OntheotherhandBag-1waspositivelyrelatedtothetumorgradetumorstageandtheprognosisofthepatientP<0.05.SignificantcorrelationwasnotedbetweenBcl-2andBag-1r=0.438.CONCLUSIONBag-1mightserveasaprognosticmakerfortherenalcellcarcinoma.Bcl-2mightbeinvolvedinthetrans-formationofbenigntomalignantprocessbutisnotrelatedtothedegreeofdifferentiationofthetumorcells. 0 Bcl-2Bag-1.Bcl-2Bag-1Bcl-2Bag-1. 1 1.11989/19974158.44677.2615.WHO:G15G213G323.UICCTNM:181282.8mo5a5a70.7%.10. 1.2Bcl-2SantaCruzBag-1Dr.H.BlissMurphySABC.5m.SABCBcl-2Bag-1.Bcl-2Bag-1150SABC1100.150Bcl-2Bag-14IgGSABCDABPBS.Bcl-2Bag-1.10405100.Bcl-2:<30%+30%50%>50%.Bag-1<10%+10%30%>30%. :2tBcl-2Bag-1r. 2 2.1Bcl2Bag1 2.1.1Bcl-2103730.0%.41311075.6%Bcl-2.Bcl-22P<0.05.Bcl-2261973.1%151280.0%2P>0.05Bcl-2Fig1.2.1.2Bag-1Bag-1.Bag-110110.0%2561.0%2P<0.05.Bag-1261661.5%15960.0%2P>0.05Bag-1Fig2. 2.2Bcl2Bag1 2.2.1WHOG15G213G323.Bcl-2tG1G2:P>0.05G2G3:P>0.05G1G3:P>0.05.Bag-1G1G2:P<0.05G2G3:P<0.05G1G3:P<0.05Tab1.Bcl-2;Bag-1. 12 1Bcl-2Bag-1 2.2.2UICCTNM181282.Bcl-2tP>0.05.Bag-1P<0.05Tab1.Bcl-2;Bag-1. 2.2.38mo5a5a5a2970.7%.t:Bcl-2P>0.05Bag-1P<0.05Bag-1Tab1. 2.3Bcl2Bag141Bcl-2Bag-1r=0.438Fig3.Bcl-2Bag-1. 3 3 Bcl-2.12.Hofmockel331Bcl-2.4.mAb.Bcl-2Bcl-2.Bcl-2. Bag-156. Bag-1Bag-1.Bag-1.Bag-1.Bag-1Bcl-2Bag-1Bcl-2Bcl-2.41Bcl-2Bag-1r=0.438.Bcl-2Bag-1. : 1QiaoWHuJLWangJB.TheeffectofTGF1abouttheex-pressionofgastricadenocarcinomacellSGC-7901J.Di-siYunyiDaxueXuebaoJFourthMilMedUniv1998;195:539-540. 2FanYDLiKZZhaoYT.Themorphologicaltransformofapoptosisinhumancholangiocarcinomacellsinducedbybcl-2mRNA-cleavingribozymeJ.Di-siJunyiDaxueXuebaoJFourthMilMedUniv2000;213:324-327. 3HofmockelGWittmannADammrichJBassukasID.Expres-sionofp53andbcl-2inprimarylocallyconfinedrenalcarcino-mas:NoevidenceforprognosticsignificanceJ.AnticancerRes1996;11:3807-3811. 4TangQHWangHChenBQCongYPShaoGXLeiFWangWY.Experssionandsignificanceofanti-apoptosisgenebcl-2inrenalcarcinomaJ.Di-siJunyiDaxueXuebaoJFourthMilMedUniv1999;202:141-145. 5TangSCShahetaNChernenkoGKhalifaMWangX.Ex-pressionofBAG-1ininvasivebreastcarcinomasJ.JClinOn-col1999;