常用荧光团的参数与选择.docx

常用荧光团的参数与选择2015/4/17FluorophorePeakExcitation (nm)PeakEmission (nm)Intersection (nm)TypeCenterWavelength(nm)Optical Density ODBandwith(nm)MinimumTransmission(%)1,8-ANS (BSA)374.9477422.9Excitation37765085Emission480310452-dodecylresorufin (lipid)581.9595589.5Excitation58031045Emission593640905-(and-6)-carboxy-2,7-dichlorofluorescein (pH 9.0)504524.9513.8Excitation50531045Emission520635905-carboxytetramethylrhodamine 5-TAMRA (MeOH)542.9567554.4Excitation54362290Emission568310455-chloromethylfluorescein (pH 7.2)490.9517501.8Excitation49231045Emission515310455-FAM (pH 9.0)491.9518.1503.5Excitation49231045Emission520635905-ROX (pH 7.0)578.2604589.7Excitation58031045Emission600380605-TAMRA (pH 7.0)553576563.4Excitation55038060Emission580310456-carboxyrhodamine 6G (pH 7.0)525547536Excitation52063590Emission546310456-JOE (pH 9.0)519.8548532.7Excitation52063590Emission550380607-amino-4-methylcoumarin (pH 7.0)346442396.5Excitation34062675Emission442310407-aminoactinomycin D (DNA)548.1647.9601.8Excitation55038060Emission647310457-hydroxy-4-methylcoumarin (pH 9.0)360.1447.9403Excitation36531025Emission44766090Acridine orange (DNA)502.1526513Excitation50062490Emission53164090Alexa Fluor 350 goat anti-mouse IgG antibody (pH 8.0)343441392.9Excitation34062675Emission44231040Alexa Fluor 405 goat anti-mouse IgG antibody (pH 7.2)401.1422.1409Excitation40035055Emission42031040Alexa Fluor 430 goat anti-mouse IgG antibody (pH 7.2)431.2540492.9Excitation43031040Emission54031045Alexa Fluor 488 dye-labeled oligonucleotide (H2O)493.1519505.8Excitation49231045Emission52063590Alexa Fluor 488 goat anti-mouse IgG antibody (pH 8.0)498.9520508.8Excitation50062490Emission52063590Alexa Fluor 488 hydrazide (H2O)492.8517.1504.9Excitation49231045Emission51531045Alexa Fluor 514 goat anti-mouse IgG antibody (pH 8.0)518542.9529.8Excitation52063590Emission54362290Alexa Fluor 532 goat anti-mouse IgG antibody (pH 7.2)533.9553543Excitation53531045Emission55038060Alexa Fluor 546 dye-labeled oligonucleotide (H2O)554.1574563.8Excitation55038060Emission58031045Alexa Fluor 546 goat anti-mouse IgG antibody (pH 7.2)561.3572566.9Excitation56264090Emission56831045Alexa Fluor 555 dye-labeled oligonucleotide (H2O)552.3567.1559.6Excitation55038060Emission56831045Alexa Fluor 555 goat anti-mouse IgG antibody (pH 7.2)553.2567.9560.2Excitation55038060Emission56831045Alexa Fluor 555 hydrazide (H2O)551.1564557.1Excitation55038060Emission56264090Alexa Fluor 568 goat anti-mouse IgG antibody (pH 7.2)578.9603590.2Excitation58031045Emission60038060Alexa Fluor 568 hydrazide (H2O)572.9599.9585.8Excitation56831045Emission60038060Alexa Fluor 594 cadaverine (H2O)587.9614.1600.1Excitation58931045Emission61031045Alexa Fluor 594 dye-labeled oligonucleotide (H2O)590.9621605.7Excitation58931045Emission62031045Alexa Fluor 594 goat anti-mouse IgG antibody (pH 7.2)590.2618.1604.2Excitation58931045Emission62031045Alexa Fluor 610 goat anti-mouse IgG antibody (pH 7.2)611.9630620.2Excitation61031045Emission62864090Alexa Fluor 610-R-phycoerythrin streptavidin (pH 7.2)566.9627589.7Excitation56831045Emission62864090Alexa Fluor 633 goat anti-mouse IgG antibody (pH 7.2)631.1649.9640.2Excitation63241085Emission65041085Alexa Fluor 635 goat anti-mouse IgG antibody (pH 7.2)633.1647.9639.9Excitation63241085Emission64731045Alexa Fluor 647 dye-labeled oligonucleotide (H2O)650.1667.9659.1Excitation65041085Emission67141085Alexa Fluor 647 goat anti-mouse IgG antibody (pH 7.2)649.9671660.6Excitation65041085Emission67141085Alexa Fluor 647 hydrazide (H2O)649.1670659.2Excitation65041085Emission67141085Alexa Fluor 647-R-phycoerythrin streptavidin (pH 7.2)568.2666648.9Excitation56831045Emission67141085Alexa Fluor 660 goat anti-mouse IgG antibody (pH 7.2)663.1690.9679.3Excitation65641085Emission69031050Alexa Fluor 680 goat anti-mouse IgG antibody (pH 7.2)680.9703.9692.8Excitation67631050Emission70038060Alexa Fluor 680-R-phycoerythrin streptavidin (pH 7.2)569.6702687.1Excitation56831045Emission70038060Alexa Fluor 700 goat anti-mouse IgG antibody (pH 7.2)695.9719708.3Excitation69431050Emission73031050Alexa Fluor 750 goat anti-mouse IgG antibody (pH 7.2)752.4776.1763.6Excitation76631050Emission78031050Alexa Fluor 790 goat anti-mouse IgG antibody (pH 7.2)781.8804.9791.1Excitation78031050Emission80031050Alexa Fluorr 350346.9443.9394.2Excitation34062675Emission44231040Allophycocyanin (pH 7.5)651660.1655.6Excitation65041085Emission65641085Amplex UltraRed peroxidation product (pH 7.5)567.9581575.2Excitation56831045Emission58031045BOBO-1 (DNA)461.2483.9472.6Excitation45831045Emission48363290BOBO-3 (DNA)570.2605.1590.2Excitation56831045Emission61031045BODIPY 630-650-X (MeOH)625.2641633.7Excitation62464090Emission64031045BODIPY 650-665-X (MeOH)645.9664.1654.9Excitation64731045Emission67141085BODIPY FL (MeOH)502.1511.1506.7Excitation50062490Emission51068490BODIPY FL goat anti-mouse IgG antibody (pH 7.2)505.3512504.1Excitation50531045Emission51068490BODIPY R6G (MeOH)528.2546.9539.2Excitation53164090Emission54631045BODIPY TMR-X (MeOH)544570.1559.3Excitation54362290Emission56831045BODIPY TMR-X goat anti-mouse IgG antibody (pH 7.2)544573561.4Excitation54362290Emission56831045BODIPY TR-X (MeOH)588621607.2Excitation58931045Emission62031045BODIPY TR-X phallacidin (pH 7.0)590621608.1Excitation58931045Emission62031045Calcein (pH 9.0)493.9514503.6Excitation49231045Emission51531045Calcium Crimson (Ca2+)589608.9598.3Excitation58931045Emission61031045Calcium Green-1 (Ca2+)507529.1517Excitation50831045Emission53164090Calcium Orange (Ca2+)549.1574561.3Excitation55038060Emission56831045Carboxynaphthofluorescein (pH 10.0)598.9674637Excitation60038060Emission67631050Cascade Blue BSA (pH 7.0)401.1419407.8Excitation40035055Emission42031040Cascade Yellow goat anti-mouse IgG antibody (pH 8.0)399.1549.1472.4Excitation40035055Emission55038060CellMask Deep Red plasma membrane stain659675.9668.6Excitation65641085Emission67631050CellMask Orange plasma membrane stain556571.9563.5Excitation55038060Emission56831045CellTrace BODIPY TR methyl ester (lipid)597.9624.9613.5Excitation60038060Emission62464090CellTrace calcein violet (pH 7.2)400.1452.1427.7Excitation40035055Emission45038060CellTracker Red CMTPX586614597.1Excitation58931045Emission61031045CellTracker Violet BMQC+GSH404.1525.9470.2Excitation40541085Emission53164090CFP (pH 7.4)433.1475.1465.3Excitation43631040Emission47263090CMR (pH 7.4)547574.9560.5Excitation54631045Emission58031045Cy3 dye-labeled IgG antibody (pH 7.2)553.9565.8559.5Excitation55038060Emission56831045Cy5 dye-labeled IgG antibody (pH 7.2)648.9666.1657.9Excitation65041085Emission67141085CyQUA GR (DNA)502.1522.9512.2Excitation50062490Emission52063590DAF-FM (NO)495519505.4Excitation49231045Emission52063590DAPI (DNA)359460.9401.1Excitation36531025Emission45831045Dapoxyl (2-aminoethyl)sulfonamide (MeOH)373.1578.9459.2Excitation37765085Emission58031045DDAO (pH 9.0)647.7657.1653.4Excitation64731045Emission65641085Di-8-ANEPPS (lipid)467631536.9Excitation46731045Emission63241085DiA (lipid)456.9585.9521.5Excitation45831045Emission58931045DiD (lipid)647.9669.9658.3Excitation64731045Emission67141085DiFMU (pH 3.0)319.9451.1370.4Excitation33431025Emission45038060DiFMU (pH 9.0)358450406.5Excitation36531025Emission45038060DiI (lipid)550.9568.9559.6Excitation55038060Emission56831045DiO (lipid)489.2506497.8Excitation48831045Emission50531045DiR (lipid)749.9782764.7Excitation76631050Emission78031050EGFP (pH 7)488.9508.1500.1Excitation48831045Emission50831045ELF 97 alcohol (pH 8.0)300536405.2Excitation33431025Emission53664090Eosin goat anti-mouse IgG antibody (pH 8.0)525545.9535.5Excitation52063590Emission54631045ER-Tracker Blue-White DPX (lipid)372.1556.8422.2Excitation37765085Emission56264090Ethidium bromide (DNA)300603550.2Excitation33431025Emission60038060Ethidium homodimer-1 (DNA)527616.9574.3Excitation53164090Emission62031045FITC goat anti-mouse IgG antibody (pH 8.0)495.1519506.5Excitation49231045Emission52063590FlAsH-CCPGCC511530.1520.1Excitation51068490Emission53164090Fluo-3 (Ca2+)506527516.1Excitation50531045Emission53164090Fluo-4 (Ca2+)494515.9504.3Excitation49231045Emission51531045Fluorescein (pH 13.0)492.9513502.1Excitation49231045Emission51531045Fluorescein (pH 9.0)489.9513.2501.1Excitation48831045Emission51531045Fluorescein dextran (pH 8.0)501.1524511.8Excitation50062490Emission52063590Fluorescein goat anti-mouse IgG antibody (pH 8.0)497.9517507.1Excitation50062490Emission51531045FluoSpheres blue fluorescent microspheres (H2O)355.9414383.8Excitation36531025Emission41531040FluoSpheres crimson fluorescent microspheres (H20)619.9646.1635.3Excitation62031045Emission64731045FluoSpheres dark red fluorescent microspheres (H2O)656683.1671.4Excitation65641085Emission69031050FluoSpheres orange fluorescent microspheres (H2O)540559.1551.3Excitation54031045Emission56264090FluoSpheres red fluorescent microspheres (H2O)576.1607594.5Excitation58031045Emission61031045FluoSpheres yellow-green fluorescent microspheres (H2O)503.1513.9508.1Excitation50531045Emission51531045FluxOR thallium indicator (pH 7.2)492.9521505.2Excitation49231045Emission52063590FM 1-43 (lipid)473579522.9Excitation47263090Emission58031045FM 4-64 (2% CHAPS)505725.1621.1Excitation50531045Emission73031050FxCycle Far Red stain640.2658650.1Excitation64031045Emission65641085FxCycle Violet stain359460.9401.1Excitation36531025Emission45831045HCS CellMask Blue stain346.9443.9394.2Excitation34062675Emission44231040HCS CellMask Deep Red stain649.1670659.2Excitation65041085Emission67141085HCS CellMask Green Stain492.8517.1504.9Excitation49231045Emission51531045HCS CellMask Orange stain551.1564557.1Excitation55038060Emission56264090HCS CellMask Red cytoplasmic (nuclear stain)621.9644632.6Excitation62031045Emission64731045HCS CellMask Red stain587.9614.1600.1Excitation58931045Emission61031045HCS LipidTOX Deep Red neutral lipid stain (triglyceride)634.9652644.1Excitation63641085Emission65041085HCS LipidTOX Green neutral lipid stain (triglyceride)498507502.2Excitation50062490Emission50831045HCS LipidTOX Green phospholipidosis detection reagent504536518.2Excitation50531045Emission53664090HCS LipidTOX Red neutral lipid stain (triglyceride)581.9615.9601.8Excitation58031045Emission62031045HCS LipidTOX Red phospholipidosis detection reagent584.2608596.3Excitation58031045Emission61031045HCS NuclearMask Deep Red stain (DNA)637.9686.1663.1Excitation63641085Emission69031050HCS NuclearMask Red stain621.9644632.6Excitation62031045Emission64731045Hoechst 33258 (DNA)352454.9394.9Excitation34062675Emission45531045JOJO-1 (DNA)530544536.2Excitation53164090Emission54362290LDS 751 (DNA)560.1711.1649Excitation56264090Emission70038060LIVE DEAD Fixable Aqua Dead Cell Stain (pH 7.2)367526.1440Excitation36531025Emission53164090LIVE DEAD Fixable Blue Dead Cell Stain343441392.9Excitation34062675Emission44231040LIVE DEAD Fixable Far Red Dead Cell Stain649.9671660.6Excitation65041085Emission67141085LIVE DEAD Fixable Green Dead Cell Stain497.9525.9510.1Excitation50062490Emission53164090LIVE DEAD Fixable Near-IR Dead Cell Stain752.4776.1763.6Excitation76631050Emission78031050LIVE DEAD Fixable Red Dead Cell Stain595613603.9Excitation59364090Emission61031045LIVE DEAD Fixable Violet Dead Cell Stain403.9454.9431.4Excitation40541085Emission45531045LIVE DEAD Fixable Yellow Dead Cell Stain (pH 7.2)400.1550.9469.4Excitation40035055Emission55038060LOLO-1 (DNA)568.1580.1574.4Excitation56831045Emission58031045Lucifer yellow CH (H2O)427.9544482.7Excitation43031040Emission54362290LysoSensor Blue (pH 5.0)395424397.6Excitation39431030Emission42031040LysoSensor Green (pH 5.0)446.8502483.2Excitation44766090Emission50062490LysoTracker Blue (MeOH)373421.8396.4Excitation37765085Emission42031040LysoTracker Green (pH 5.2)500.9509.1505.2Excitation50062490Emission51068490LysoTracker Red (pH 5.2)573.1592582.6Excitation56831045Emission59364090LysoTracker Yellow HCK-123488565509Excitation48831045Emission56831045Magnesium Green (Mg2+)507531517.2Excitation50831045Emission53164090Marina Blue goat anti-mouse IgG antibody (pH 8.0)363.8461.1412Excitation36531025Emission45831045mBBr+GSH (pH 8.0)394490.1449.2Excitation39431030Emission49231045MitoTracker Deep Red FM (MeOH)641661.9652.2Excitation64031045Emission65641085MitoTracker Green FM (MeOH)490.1512502Excitation49231045Emission51068490MitoTracker Orange (MeOH)551.2575562.1Excitation55038060Emission58031045MitoTracker Red (MeOH)578.2598.1587.3Excitation58031045Emission60038060mRFP (pH 7.4)554.8583.2565.1Excitation55038060Emission58031045NBD-X (MeOH)467537.9499.2Excitation46731045Emission53664090NeuroTrace 500-525 green fluorescent Nissl stain (RNA)497524508.2Excitation50062490Emission52063590Nile blue (EtOH)630.6660649Excitation63241085Emission65641085Nile red (phospholipid)553636.9602.5Excitation55038060Emission63641085Nile red (triglyceride)510.1583.1556Excitation51068490Emission58031045OFP546.9567556.8Excitation54631045Emission56831045Oregon Green 488 DHPE504536518.2Excitation50531045Emission53664090Oregon Green 488 goat anti-mouse IgG antibody (pH 8.0)497.9525.9510.1Excitation50062490Emission53164090Oregon Green 514 goat anti-mouse IgG antibody (pH 8.0)512.8533521.6Excitation51531045Emission53241085Pacific Blue dye-labeled oligonucleotide (H2O)402454.9430.5Excitation40035055Emission45531045Pacific Blue goat anti-mouse IgG antibody (pH 8