岛津2010AHT高效液相色谱仪进样量准确度校正.docx

岛津2010AHT高效液相色谱仪进样量准确度校正原理使用装试样（水）1.5mL的试样瓶，测定进样量为50L的10次注入动作的注入前和注入后的重量，由重量差计算进样量准确度。测定后，根据测定结果，可自动校正进样量。合格基准为2.0%，即98%102%。1 实验材料1.1 仪器高效液相色谱仪（LC-2010AHT，岛津），电子天平（AB135-S，梅特勒），超纯水仪（Exceed-E-UV，艾柯），温度计。1.2 试剂甲醇（西格玛，色谱纯），超纯水。2 方法（1）1.5mL试样瓶内装1mL水，用天平测定重量W1。（2）将试样瓶放到架1的1号位置上。（3）按 F 4 CALIB，显示输入System Pass-ID-NO用的如图画面。（4）输入System Pass-ID-NO。（数字键）（5）按 F 2 OK。显示如图画面。 （6）选择“H/W Calibration”。，显示Hardware Calibration画面。（7）按。显示Injection Vol画面。（8）输入顺序 1 测定的数值。（数字键，）。（9）按 F 2 NEXT。开始注入，切换至下一画面。（10）等待注入结束。10次注入结束时，切换下一画面。（11）取下试样瓶，用天平测定重量。（12）输入顺序11测定的数值。（数字键，）。（13）按 F 2 NEXT时，计算进样量准确度，显示结果。（14）根据测定结果校正时，按 F 4 APPLY。校正进样量修正参数。（15）按 F 5 EXIT。返回至显示Hardware Calibration画面。（16）确认校正正确时，执行“进样量准确度的确认”（自动进样器）。1990年国际温标纯水密表(kg/m3)t90()0.00.10.20.30.40.50.60.70.80.90999.840999.846999.853999.859999.865999.871999.877999.883999.888999.8931999.898999.904999.908999.913999.917999.921999.925999.929999.933999.9372999.940999.943999.946999.949999.952999.954999.956999.959999.961999.9623999.964999.966999.967999.968999.969999.970999.971999.971999.972999.9724999.972999.972999.972999.971999.971999.970999.969999.968999.967999.9655999.964999.962999.960999.958999.956999.954999.951999.949999.946999.9436999.940999.937999.934999.930999.926999.923999.919999.915999.910999.9067999.901999.897999.892999.887999.882999.877999.871999.866999.880999.8548999.848999.842999.836999.829999.823999.816999.809999.802999.795999.7889999.781999.773999.765999.758999.750999.742999.734999.725999.717999.70810999.699999.691999.682999.672999.663999.654999.644999.634999.625999.61511999.605999.595999.584999.574999.563999.553999.542999.531999.520999.50812999.497999.486999.474999.462999.450999.439999.426999.414999.402999.38913999.377999.384999.351999.338999.325999.312999.299999.285999.271999.25814999.244999.230999.216999.202999.187999.173999.158999.144999.129999.11415999.099999.084999.069999.053999.038999.022999.006998.991998.975998.95916998.943998.926998.910998.893998.876998.860998.843998.826998.809998.79217998.774998.757998.739998.722998.704998.686998.668998.650998.632998.61318998.595998.576998.557998.539998.520998.501998.482998.463998.443998.42419998.404998.385998.365998.345998.325998.305998.285998.265998.244998.22420998.203998.182998.162998.141998.120998.099998.077998.056998.035998.01321997.991997.970997.948997.926997.904997.882997.859997.837997.815997.79222997.769997.747997.724997.701997.678997.655997.631997.608997.584997.56123997.537997.513997.490997.466997.442997.417997.393997.396997.344997.32024997.295997.270997.246997.221997.195997.170997.145997.120997.094997.06925997.043997.018996.992996.966996.940996.914996.888996.861996.835996.80926996.782996.755996.729996.702996.675996.648996.621996.594996.566996.53927996.511996.484996.456996.428996.401996.373996.344996.316996.288996.26028996.231996.203996.174996.146996.117996.088996.059996.030996.001996.97229995.943995.913995.884995.854995.825995.795995.765995.753995.705995.67530995.645995.615995.584995.554995.523995.493995.462995.431995.401995.37031995.339995.307995.276995.245995.214995.182995.151995.119995.087995.05532995.024994.992994.960994.927994.895994.863994.831994.798994.766994.73333994.700994.667994.635994.602994.569994.535994.502994.469994.436994.40234994.369994.335994.301994.267994.234994.200994.166994.132994.098994.06335994.029993.994993.960993.925993.891993.856993.821993.786993.751993.71636993.681993.646993.610993.575993.540993.504993.469993.433993.397993.36137993.325993.280993.253993.217993.181993.144993.108993.072993.035992.99938992.962992.925992.888992.851992.814992.777992.740992.703992.665992.62839992.591992.553992.516992.478992.440992.402992.364992.326992.288992.25040992.212991.826991.432991.031990.623990.208989.786987.358988.922988.47950988.030987.575987.113986.644986.169985.688985.201984.707984.208983.70260983.191982.673982.150981.621981.086980.546979.999979.448978.890978.32770977.759977.185976.606