硫酸铵饱和度的常用表,聚丙烯酰胺凝胶的配制表.doc

硫酸铵饱和度的常用表1调整硫酸铵溶液饱和度计算表（0）在 0 硫 酸 铵 终 浓 度，% 饱 和 度硫 酸 铵 初 浓 度 ， 饱 和 度20253035404550556065707580859095100每 100ml 溶 液 加 固 体 硫 酸 铵 的 克 数010.613.416.419.422.625.829.132.636.139.843.647.651.655.960.365.069.757.910.813.716.619.722.926.229.633.136.840.544.448.452.657.061.566.2105.38.110.913.916.920.023.326.630.133.737.441.245.249.353.658.162.7152.65.48.211.114.117.220.423.727.130.634.338.142.046.050.354.759.22002.75.58.311.314.317.520.724.127.631.234.938.742.746.951.255.7250 2.75.68.411.514.617.921.124.528.031.735.539.543.647.852.23002.85.68.611.714.818.121.424.928.532.336.240.244.548.83502.85.78.711.815.118.421.825.429.132.936.941.045.34002.95.88.912.015.318.722.225.829.633.537.641.84502.95.99.012.315.619.022.626.330.234.238.35003.06.09.212.515.919.423.026.830.834.85503.06.19.312.716.119.723.527.331.36003.16.29.512.916.420.123.127.96503.16.39.713.216.820.524.47003.26.59.913.417.120.97503/26.610.113.717.48003.36.710.313.98503.46.810.59003.47.09503.510002调整硫酸铵溶液饱和度计算表（25）在 25 硫 酸 铵 终 浓 度 ，% 饱 和 度硫 酸 铵 初 浓 度 ， 饱 和 度10202530333540455055606570758090100每 1000ml 溶 液 加 固 体 硫 酸 铵 的 克 数056114144176196209243277313351390430472516561662767105786118137150183216251288326365406449494592694202959789112315518922526230034038242452061925304961931251581932302673073483904855833019306294127162198235273314356449546331243741071421772142522923334265223531639412916420023827831941150640316397132168205245285375469453265991341712102503394315033661011371762143023925533671031411792643536034691051432273146534701071902757035721532377536115198807715790793不同温度下饱和硫酸铵溶液的数据温 度（）010202530重量百分数41.4242.2243.0943.4743.85摩尔浓度3.93.974.064.104.13每1000g水中含硫酸铵摩尔数5.355.535.735.825.911000ml水中用硫酸铵克数706.8730.5755.8766.8777.5每1000ml溶液中含硫酸铵克数514.8525.2536.5541.2545.9聚丙烯酰胺凝胶的配制表1 配制Tris-甘氨酸SDS-PAGE聚丙烯酰胺凝胶电泳分离胶所用溶液溶液成分不同体积（ml）凝胶液中各成分所需体积（ml）5101520253040506% 水2.65.37.910.613.215.921.226.5 30%丙烯酰胺溶液123456810 1.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.5 10% SDS0.050.10.150.20.250.30.40.5 10%过硫酸氨0.050.10.150.20.250.30.40.5 TEMED0.0040.0080.0120.0160.020.0240.0320.048% 水2.34.66.99.311.513.918.523.2 30%丙烯酰胺溶液1.32.745.36.7810.713.3 1.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.5 10% SDS0.050.10.150.20.250.30.40.5 10%过硫酸氨0.050.10.150.20.250.30.40.5 TEMED0.0030.0060.0090.0120.0150.0180.0240.0310% 水1.945.97.99.911.915.919.8 30%丙烯酰胺溶液1.73.356.78.31013.316.7 1.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.5 10% SDS0.050.10.150.20.250.30.40.5 10%过硫酸氨0.050.10.150.20.250.30.40.5 TEMED0.0020.0040.0060.0080.010.0120.0160.0212% 水1.63.34.96.68.29.913.216.5 30%丙烯酰胺溶液246810121620 1.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.5 10% SDS0.050.10.150.20.250.30.40.5 10%过硫酸氨0.050.10.150.20.250.30.40.5 TEMED0.0020.0040.0060.0080.010.0120.0160.0215% 水1.12.33.44.65.76.99.211.5 30%丙烯酰胺溶液2.557.51012.5152025 1.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.5 10% SDS0.050.10.150.20.250.30.40.5 10%过硫酸氨0.050.10.150.20.250.30.40.5 TEMED0.0020.0040.0060.0080.010.0120.0160.02表2 配制6% Tris-甘氨酸SDS-PAGE聚丙烯酰胺凝胶电泳5%积层胶所用溶液溶液成分不同体积（ml）凝胶液中各成分所需体积（ml）123456810 水0.681.42.12.73.44.15.56.8 30%丙烯酰胺溶液0.170.330.50.670.8311.31.7 1.5 mol/L Tr