BG11液体培养基.doc

BG11液体培养基BG11液体培养基就是： Stock1 定容100mL 柠檬酸 0.3g 柠檬酸铁胺 0.3g EDTANa2 0.05g Stock2 定容1000mL NaNO3 30g K2HPO4 0.78g MgSO47H2O 1.5g Stock3 定容100mL CaCl22H2O 1.9g Stock4 定容100mL Na2CO3 2g Stock5 定容1000mL H3BO3 2.86g MnCl24H2O 1.81g ZnSO47H2O 0.222g Na2MnO42H2O 0.391g CuSO45H2O 0.079g Co(NO3)26H2O 0.049g Stock1 取用2mL Stock2 取用20mL Stock3 取用2mL Stock4 取用1mL Stock5 取用1mL 总定容 1000mL D1 (Diatom medium)Component Amount Stock Solution (1) NaNO3 1 mL/L12 g/100ml dH2O (2) K2HPO4 1 mL/L 4 g/100 mL dH2O (3) MgSO47H2O1 mL/L 7g/100 mL dH2O (4 ) CaCl22H2O1 mL/L 2 g/100 mL dH2O (5 ) KH2PO4 1 mL/L 8 g/100 mL dH2O (6 ) Na2SiO3.9H2O1 mL/L 10g/100ml dH2O(7) MnSO4 0.1mL/L0.2g/100ml dH2O(8) 柠檬酸铁 1 mL/L 0.5g/100ml dH2O(9) A5 （Trace mental solution） 1ml/L H3BO3 2.86g/L dH2O MnCl24H2O1.86g/L dH2O ZnSO47H2O 0.22g/L dH2O Na2MoO4.2H2O0.39g/L dH2O CuSO4. 5 H2O0.08g/L dH2O Co(NO3)2.6 H2O0.05g/L dH2O(10) 土壤提取液 20 mL/L 土壤提取液配制方法:取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时，冷却，沉淀24小时，此过程连续进行3次，然后过滤，取上清液，于高压灭菌锅中灭菌后于4冰箱中保存备用。f/2 mediumComponent Amount (1) NaNO3 75 mg/L(2) NaH2PO4 2H2O 0.6 mg/L(3) Vitamin B12 0.5 g/L (4) Biotin 0.5 g/L (5) Thiamine HCl 100g/L (6) Na2SiO3 9H2O10 mg/L (7) f/2 metals 1 mL/L Na2EDTA 2H2O 4.4g FeCl3 6H2O 3.16 g CoSO4 7H2O 0.012g ZnSO4 7H2O 0.021g MnCl2 4H2O 0.18 g CuSO4 5H2O 0