大叶柴胡鉴别.doc

ReportProject: Bupleurum longiradiatum（大叶柴胡）Customer name and address: YUAN Ye / NKI-TCMProject objective: Identification Bupleurum longiradiatumDate: 6th May 2012Laboratory: NKI-TCM1. TLCTest solution. Heat 0.5 g of the powdered drug in 30ml of methanol R under a reflux condenser for 10 min. Cool then filtered and evaporates the solvent to dryness. Take up the residue in 5 mL of the methanol. Reference solution. Dissolve 1 mg of Saikosaponin D R in 1 ml of methanol R.Plate: TLC silica gel plate R (2-10 m).Mobile phase: ethyl acetate R, ethanol R, water R (8:2:1 V/V). Application: 10 L as bands.Development: over a path of 8 cm.Drying: in airDetection: spray the warm plate with 2g/100ml p-dimethyl aminobenzaldehyde in 40% sulfuric acid and dry at 60 for 20min. Examine in ultraviolet light at 365 nm.Left: Bupleurum longiradiatum Right: radix bupleuriDetection under sunlightLeft: Bupleurum longiradiatum Right: radix bupleuriExamine in ultraviolet light at 365 nm2. Ultraviolet spectrophotometryTest solution. 0.15g of the powdered drug (355) in 50 ml of ethanol R sonicate at room time for 10 minutes, filter and dilute to 100.0ml with ethanol R.Wavelength range: 200-350.Instrument Type: UV-2500PC seriesResult: Sample contains three characteristic absorption peaks: 336.8, 315.8, and 297.0. But radix bupleuri don not have them.Red: Sample ( Bupleurum longiradiatum)Green: ra