09-10分子生物学期末试卷.doc

福建农林大学期末考试试卷20092010学年第 学期课程名称： 分子生物学 考试时间 120 分钟 专业 年级 学号 姓名 题号一二三四总得分得分评卷人签字复核人签字得分一、填空题（每空0.5分，共10分）1、There are five types of DNA repair, include photo-reactivation repair, _， _，_ and error-prone repair. 2、The holoenzyme of E.coli RNA polymerase is_，the core enzyme is made up of _，while the_ factor has the ability to recognize specific binding sites.3、The eukaryotes have a well-defined cell cycle.The phase are：G1，_，_ and M.4、Only one strand of a DNA duplex will be transcribed. So the RNA is identical in sequence with another strand of the DNA which is called _，and the template strand is _.5、In eukaryotes, mRNA processing includes the following steps: addition of at 5end, addition of at 3end, removal of , joining together the ,And these take place in . 6、The structure is a common secondary structural reprensentation of tRNA molecules. The tertiary structure of tRNA molecules is . 7、In the large 60S subunit of the eukaryote ribosome, there are rRNA, and rRNA, and rRNA. 得分二、名词解释（每小题2分，共30分）1、Promoter： 2、Semi-conservative replication： 3、Intron： 4、Transversion： 5、Gene recombination： 6、Hybridization： 7、Transcription：8、Proofreading activity： 9、RNA processing： 10、Triplet code：11、Chaperone： 12、Trans-acting element： 13、Open reading frame： 14、Ribozyme： 15、Zinc finger： 得分三、单项选择题（每小题1分，共15分）（ ）1、What is the percentage of A in a piece of RNA if the percentage of C is 30%?A. 30% B. 20%C. 60% D. Unable to calculate with the information provided.（ ）2、Which of the following is NOT a function of RNA?A. It acts a template during translation.B. It is required for RNA splicing.C. It is essential for the structure of ribosomes.D. It attaches the correct amino acid to tRNA.（ ）3、Histones are found in close association with DNA. Which of the following statements is incorrect?A. Histone H1 is less evolutionarily conserved than core histones.B. A nucleosome is composed of 146 bp of DNA and a histone octomerC. There are only four types of histonesD. Nucleosomes are evenly distribution within chromatin（ ）4、The elongation of the lagging strand during DNA synthesis:A. In the 3-5 directionB. Produces Okazaki fragments C. Polymerizes into the direction of fork movementD. Does not require a template strand（ ）5、Telomeres are found at the end of chromosomes and they are synthesized by telomerase. Which one of the following statements is correct?A. The function of telomerase is to directly elongate the leading strand during DNA replication.B. Telomers code for ribosomal RNA.C. Telomerase is an unusual enzyme made up of RNA template and protein.D. Telomerase is active in all cell types.（ ）6、Which of the following is correct concerning transcription and DNA replication?A. Only transcription requires a primer.B. Transcription starts at the origin of replication.C. In DNA replication, both the coding and template strands are copied.D. The entire genome is copied in transcription.（ ）7、Which of the following statements describes a similarity between the structure of DNA and RNA? In both DNA and RNA, A. the nitrogenous base uracil is found.B. there is a hexose sugar.C. the carbon 2 has an hydroxyl group.D. the nitrogenous base is attached to carbon 1.（ ）8、Regulation of the trytophan operon involvesA. repression (at an operator) B. attenuationC. the concentration of free trytophan D. all of the above（ ）9、Which of the following is not stop codon? A. TAA B. TAT C. TAG D. TGA（ ）10、The Shine-Dalgarno sequence in mRNAsA. base pairs to the 3-end of E. coli 16S rRNAB. base pairs to the 5-end of E. coli 16S rRNAC. has a consensus sequence of YNCURACD. is located just downstream of the initiation codon （ ）11、Which of the following is not bound to the aminoacyl-tRNA synthetase? A. amino acid B. tRNA C. GTP D. ATP （ ）12、The ribosome scanning model for translation was proposed byA. P. Sharp B. R. RobertsC. T. Cech D. M. Kozak（ ）13、Most nuclear mRNA introns begin with _ and end with _. A. U, G B. GU, NG C. AG, GU D. GU, AG （ ）14、Which of the following is the covalent linkage of ubiquitin molecule with the protein damaged? A. Gly B.Lys C. Leu D.Thr（ ）15、In nuclear mRNA splicing, the intron is released as a A. circular RNA B. linear RNA C. lariat RNA D. a “Y” RNA得分四、问答题（45分）1、Please illuminate the main content of central dogma and draw the sketch map.（8 points）2、What is the operon? How the lac operon controls gene expression?（8 points）3、Please write out the enzymes and proteins required in prokaryotic DNA replication，and explain their functions.（7 points）4、What are the characters of expression vectors?（5 points）5、Please illuminate the function of the cap structure of mature mRN