实验室常用细胞株简介.doc

实验室常用细胞株简介细胞株名称ATCC 编号细胞来源细胞种类生长状态使用培养基293CRL-1573人胎肾贴壁、上皮样MEM/NEAA,10%FBS2215无人肝癌贴壁、上皮样DMEM,15%FBS127TAgCRL-2817小鼠多能胚胎干细胞胚胎型DMEM, 10% FBS293/CHE-FcCRL-2368人肾上皮细胞、贴壁DMEM,10%FBS3T3-L1CL-173小鼠胚胎成纤维成纤维DMEM,10%FBS3T6CCL-96小鼠胚胎成纤维细胞、贴壁DMEM,10%FBSA431CRL-1555人表皮细胞癌上皮细胞、贴壁DMEM,10%FBSA549CCL-185人肺癌贴壁、上皮样F12,10%FBSA9CRL-6319小鼠结缔组织成纤维细胞、贴壁DMEM,10% FBS+AR42JCRL-1492大鼠胰腺肿瘤贴壁、上皮样HamsF-12K, 20%FBSAtT-20CCL-89小鼠垂体肿瘤上皮细胞、贴壁F-10, 15% HS和2.5% FBSB95-8CRL-2311绒猴EB病毒感染的白血病细胞成淋样RPMI-1640, 10% FBSBALB/3T3CCL-163小鼠胚胎成纤维细胞、贴壁DMEM, 10% FBSbel7402无人肝癌贴壁、上皮样RPMI-1640,15%FBSBeWoCCL-98人绒毛癌上皮细胞、贴壁F-12K, 15%F12BHK-21CCL-10仓鼠肾成纤维细胞、贴壁MEM/NEAA, 10% FBSBHL-100无人乳房上皮细胞、贴壁McCoy5A, 10% FBSBRL 3ACRL-1442大鼠肝上皮细胞、贴壁MEM/NEAA, 10% FBSBTCRL-1390牛鼻甲骨细胞成纤维细胞、贴壁DMEM, 10% FBSCaco-2HTB-37人结肠腺癌上皮细胞、贴壁MEM/NEAA, 10% FBSChang无人肝脏上皮细胞、贴壁BME, 10% FCSCHO-K1CRL-9618仓鼠卵巢上皮细胞、贴壁F-12, 10%FBSCL2CRL-2514小鼠B细胞淋巴细胞、悬浮RPMI-1640, 10% FBSCL3CRL-2515小鼠B细胞淋巴细胞、悬浮RPMI-1640, 10% FBSClone 9CRL-1439大鼠肝脏上皮细胞、贴壁F-12K, 10%FBSClone M-3CCL-53.1小鼠黑素瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBSCOS-1CRL-1650猴肾成纤维细胞、贴壁DMEM, 10% FBSCOS-3无猴肾成纤维细胞、贴壁MEM, 10% FBSCOS-7CRL-1651猴非洲绿猴肾成纤维细胞、贴壁DMEM, 10% FBSCRFKCCL-94猫肾上皮细胞、贴壁MEM/NEAA, 10% FBSCV-1CCL-70猴非洲绿猴肾成纤维细胞、贴壁MEM/NEAA, 10% FBSD-17CCL-183狗骨肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSDANCRL-2130狗骨肉瘤上皮细胞、贴壁MEM/EBSS, 8% FBSDaudiCCL-213人淋巴瘤病人血液成淋巴细胞、悬浮RPMI-1640, 10% FBSDLD-1CCL-221人结肠直肠腺癌上皮细胞、贴壁RPMI-1640, 10% FBSDSDhCRL-2131狗骨肉瘤上皮细胞、贴壁MEM/EBSS, 8% FBS DSNCRL-9939狗骨肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSDu145HTB-81人前列腺瘤贴壁、上皮样MEM/NEAA,10%FBSEB-3CCL-85人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640, 10% FBSES-D3CRL-1934小鼠多能胚胎干细胞胚胎型DMEM+ 15% FBS F9CRL-1720小鼠睾丸畸胎癌胚胎型至内胚层DMEM, 15% FBSGH1CCL-82大鼠垂体肿瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBSGH3CCL-82.1大鼠垂体肿瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBSH9HTB-176人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640, 10% FBSH9/HTLV-IIIBCRL-8543人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640, 10% FBSHaKCCL-15仓鼠肾上皮细胞、贴壁BME, 10%FCSHCT-15CCL-225人结肠直肠腺癌上皮细胞、贴壁RPMI-1640, 10% FBSHelaCCL-2人宫颈癌贴壁、上皮样MEM/NEAA,10%FBSHela S3CCL-2.2人子宫颈癌贴壁、上皮样F12,10%FBSHep-2无人喉癌上皮细胞、贴壁MEM, 10% FBS;或RPMI-1640, 10% FBSHep3BHB8064人肝癌贴壁、上皮样MEM/NEAA,10%FBSHepG2HB-8065人肝癌贴壁、上皮样MEM/NEAA,10%FBSHK-2CRL-2190人肾皮质贴壁、上皮样Keratinocyte-Serum Free Medium + 5 ng/ml rhEGFHL-60CCL-240人急性早幼粒细胞白血病悬浮、粒细胞样RPMI-1640,20%FBSHP HT1080 polyCRL-12012人纤维肉瘤上皮细胞、贴壁DMEM, 10% FBSHT-1080CCL-121人纤维肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSHT-29HTS-38人腺癌贴壁、上皮样DMEM/F12 (1:1),5%FBSHTCICLC 收录ATL95006大鼠肝脏贴壁、上皮样MEM/EBSS,10%FBSHuT 78TIB-161人T细胞淋巴瘤成淋巴细胞、悬浮IMDM, 20% FBSHUVEC无人脐带内皮细胞、贴壁F-12K, 10% FBS 和 肝素盐 100 ug/mlHX HT1080 xenoCRL-12011人纤维肉瘤上皮细胞、贴壁DMEM, 10% FBSI-10CCL-83小鼠睾丸癌上皮细胞、贴壁F-10, 15% HS和2.5% FBSIEC-6CRL-1592大鼠正常小肠上皮样、贴壁DMEM, 5% FBS,胰岛素0.1g/mlIM-9CCL-159人骨髓瘤病人骨髓成淋巴细胞、悬浮RPMI-1640, 10% FBSIMR-90CCL-186人肺成纤维细胞 、贴壁MEM/NEAA, 10% FBSJAA-F11CRL-2381小鼠B细胞淋巴样、悬浮DMEM,10% FBS和NEAAJEG-3HTB-36人绒毛膜癌上皮细胞、贴壁MEM/NEAA, 10% FBSJensenCCL-45大鼠肉瘤成纤维细胞、贴壁McCoys 5A, 5% FBSJurkatTIB-152人急性T淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBSK-562CCL-243人骨髓性的白血病成淋巴细胞、悬浮RPMI-1640, 10% FBSKBCCL-17人口腔癌上皮细胞、贴壁MEM/NEAA, 10% FBSKG-1CCL-246人红白血病病人骨髓骨髓白细胞、悬浮IMDM, 20% FBSKG-1aCCL-246.1人红白血病病人骨髓骨髓白细胞、悬浮IMDM, 20% FBSL2CCL-149大鼠肺上皮细胞、贴壁F-12K, 10%FBSL6CRL-1458大鼠骨骼肌成肌细胞贴壁DMEM, 10% FBSLLC-WRC 256CCL-38大鼠癌上皮细胞、贴壁199培养基, 5% HSLnCapCRL-1740人前列腺癌贴壁、上皮样RPMI-1640,10%FBSLO2无人正常肝细胞贴壁、上皮样DMEM,15%FBSM. dunni (Clone III8C)CRL-2017小鼠皮肤成纤维细胞、贴壁McCoys 5a , 10% FBSMARC S5CRL-2507小鼠B细胞悬浮、淋巴细胞样MEM/NEAA或DMEM, 20%FBS(灭活)MCF7HTB-22人乳腺癌上皮细胞、贴壁MEM/NEAA, 10% FBS10ug/ml 胰岛素MHCC97无人肝癌贴壁、上皮样DMEM,15%FBSMOLT-4CRL-1582人T淋巴细胞、急性淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBSNIH3T3CRL-1658小鼠胚胎贴壁、成纤维细胞DMEM,15%FBSNK-92CRL-2407人淋巴瘤成淋巴细胞、悬浮-MEM,12.5% HS和12.5% FBSNK-92MICRL-2408人淋巴瘤成淋巴细胞、悬浮-MEM,12.5% HS和12.5% FBSPANC-1CRL-1469人胰腺癌贴壁、上皮样DMEM,10%FBSPC12CRL-1721大鼠肾上腺嗜铬细胞瘤多角型、松散贴壁、易结团生长F12 ,15% HS和2.5%FBSPC3CRL-1435人前列腺腺癌早期、骨转移贴壁、上皮样F12,10%FBSRajiCCL-86人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640, 10% FBSRat2CRL-1764大鼠Rat2(TK-)克隆衍生成纤维细胞样、贴壁DMEM, 5% FBS,30g/ml BrdURS4;11CRL-1873人急性淋巴细胞白血病成淋巴细胞、悬浮RPMI-1640, 10% FBSSaos-2HTB-85人骨肉瘤上皮细胞、贴壁McCoy5A, 15% FBSSMMC7721无人肝癌贴壁、上皮样RPMI-1640,15%FBSSP2/0ACC146小鼠杂交瘤悬浮IMDM,7.5%FBSSW480CCL-228人结肠癌贴壁、上皮样10%FBS,L15SW620CCL-227人结肠癌贴壁、上皮样10%FBS+2mM L-GLU+90%L15THP-1TIB-202人急性单核系白血病、血液悬浮、单核细胞样RPMI-1640,10%FBSU937CRL-1593.2人淋巴浆母细胞瘤悬浮、单核细胞样RPMI-1640,10%FBSVeroCCL-81非洲绿猴肾贴壁、上皮样DMEM,10%FBSVero-E6CRL-1586非洲绿猴肾贴壁、上皮样MEM/EBSS,10%FBSWEHI-3TIB-68小鼠骨髓单核细胞白血病类巨噬细胞、悬浮IMDM, 10% FBSWI-38CCL-75人胚胎肺上皮细胞、贴壁BME, 10% FBSWISHCCL-25人羊膜上皮细胞、贴壁BME, 10% FBSWS1CRL-1502人胚胎皮肤上皮细胞、贴壁M