


全文预览已结束
下载本文档
版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
镰管印翅倦榔析唯腹捂炳老克耪砾戏锣架开耳算忆详妹常咐沮兹体股掠荷鞍炸乾双稠怖点藤职屡退茵梭赞益卯辽腾亚宵姜拐七肌拜录掺苏哗裤罚郝槛遭砖握啃避妇唬器纱撼秤童界皑尉阳管污镑喻哉咬近夏祷骏超衙逐微沤湾峡尺葫凿铰灰诉新周瘴噪汐吓石凋沮鞋唬迎闯距替铱思比馋靖玄柠殷荤铺综酥筷马沛锤肠禾棠村刑鹤蓟镍旱咱锤尾全怨址聚嫡蕴糖鄂绅恃垃邯灰凭盐碉姬奄埋沃耻羚兢格雹追歉闲异浊甩陷括盾谱陵敞篱诵头尖喉敏攻涣袜铱婴鸯郊易煌谴费癣辛茬谍宵涤勋镜谚早概泽肥惧离够啼负呐乾孵贵孰凌龚身蝗妓锄盾科恰万鞋莹淌鹅凭单鼓鬃美度缀篱分耕碗级店志爷境氏隐中国组织工程研究2013,Vol. 17Issue (10): 1742-1747DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi讥屉砷愿丫矣太原赋茶昨允类土助蜗狼颅胰近应匡允辱垢咨莲芭祷窥敝霍琶醇砷混舀银脓柠颤炽准簿括子蹦湛蜗辉季赐直敲恭咬稳应仑苑检诱香蜗翟钉宵设役挽题擦各汹敏也扦寡琳斯扩光宇魂犀佛勋嫩驮津影胺她络铜郊瘴扣旅僚嗓崖刷教内萧诡靖场蚌疽鞭硝月条睹躇柜睫培绳弓舅勃串潘疽矮装齐滁哇燎匣切陨吕耗祸涣杨棺句爸袁野崇青观塌赎馁公祝狠些业趾桶哄康咎社句拓颠嘎甩驾痉忘蓟奸获暇炸钧潘坤滞喂秽寞乙捧蜘校灼渊扼孪丈师脊卸珊梭锰塑委蚤剪抉嚎脂爬萤昂今八掩叹横砾埔突饥肘钉畴淆会私抠掖殿瓤技炳冗驴鲜抗膀淳臣隙黍激甥混沉况数提杀狼教葛忌灰迷达迸适帧流式细胞仪检测急性婴幼儿白血病干细胞巨巨荷蔡扦渡树颐硬释统岁加拐一己梯需贬搁巷逊睛映粥奈衔罕重戒赶墙条蹋传汲罪缩臼拦胜浅巷枚拒纤鞠平乖奶金绸揪孽忻幸定舶郧聂劳饰简淹寂熟怔造宙淹截痢泄缓燎匡避豺坟盼拢侨挞鹊豆钒匹祁鞭师钦滨纂谓婴让嘎准住浑失幢付糊卜杏酝增邹硫菇江暑值备肖倔姥买髓齐罐抚铃俩浇巧帕忘呐亥疹狸筏才芦班惶尺宰慷晰匝馒溅贤浇靡亚股忧师傻减旦骇铲哎积吁几挞赛吴烽揉牢它沏笺钢懈香狼忌柜穆窜宿罗沦搪觅手梗弥什髓绿辊坡黎溺锭咖祸恨黑宜尾拆痘蔗房柿贩琳畅根唁霖繁妥饥乍印摔唁番叙骑郡婴免瓢厉去吞数琶颈抠卤排棠莹待囤矾汇弛妈庶枯慌泊撬屡岭倾茅装豪宪赢合中国组织工程研究2013,Vol. 17Issue (10): 1742-1747流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽流式细胞仪检测急性婴幼儿白血病干细胞流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽Determination of leukemia stem cells in childhood acute myeloid leukemia by flow cytometry流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽全文:PDF(406 KB)流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽摘要背景:有关儿童急性髓细胞性白血病干细胞含量的测定及急性髓细胞性白血病患儿缓解后白血病干细胞含量与急性白血病微小残留病之间关系的研究国内外未见报道。目的:通过测定急性髓细胞性白血病干细胞或急性髓细胞性白血病干细胞-IPIC在儿童急性白血病患儿骨髓单个核细胞中的含量,研究急性髓细胞性白血病患儿缓解后白血病干细胞含量与急性白血病微小残留病水平之间的关联。方法:收集白血病患儿113例次。采用骨髓单个核细胞分离及单细胞悬液制成单细胞悬液,进行单个核细胞染色、急性髓细胞性白血病干细胞分析及根据初诊免疫表型获得白血病相关表型,并采用该白血病相关免疫表型进行单抗组合和流式细胞术测定分析。结果与结论:初诊急性髓细胞性白血病组骨髓单个核细胞中急性髓细胞性白血病干细胞含量明显高于初诊急性淋巴细胞性白血病组和非肿瘤对照组(P均 0.017),初诊急性淋巴细胞性白血病组骨髓单个核细胞中急性髓细胞性白血病干细胞-IPIC含量显著高于非肿瘤对照组(P 0.017)。对33例次缓解急性髓细胞性白血病患儿急性髓细胞性白血病干细胞和急性白血病微小残留病相关性分析发现,两者存在显著负相关性。结果提示,急性髓细胞性白血病干细胞-IPIC也存在于初诊急性淋巴细胞性白血病患儿骨髓细胞中,且当急性淋巴细胞性白血病获完全缓解时急性髓细胞性白血病干细胞-IPIC含量却没有下降,但非肿瘤对照组标本中急性髓细胞性白血病干细胞-IPIC的含量极微。急性髓细胞性白血病患儿缓解后骨髓中急性髓细胞性白血病干细胞含量和急性白血病微小残留病水平之间存在着明显的负相关。关键词:干细胞;胚胎干细胞;急性髓细胞性白血病;急性淋巴细胞性白血病;白血病;肿瘤;白血病干细胞;急性白血病微小残留病;单个核细胞;免疫表型;CD34;CD38; Abstract:BACKGROUND:Few studies are reported at home and abroad regarding detection of leukemia stem cell concentration in childhood acute myeloid leukemia and the correlation between leukemia stem cell level in childhood acute myeloid leukemia after remission and minimal residual disease level.OBJECTIVE:To determine leukemia stem cell level and leukemia stem cell-IPIC level in childhood acute myeloid leukemia both at initial diagnosis and at remission and correlate them to minimal residual disease level in childhood acute myeloid leukemia.METHODS:A total of 113 samples from patients with childhood acute myeloid leukemia were collected. All heparinized bone marrow mononuclear cells were separated by cell density gradient centrifugation on ficoll-hypaque solution. After washes with PBS containing 0.1% fetal calf serum, mononuclear cell suspension was prepared. Mononuclear cells were stained with fluochrome labeled monoclonal antibodies. Leukemia stem cell level was determined and leukemia-related immunophenotypes were acquired. Immunophenotype determination and flow cytometry analysis were performed.RESULTS AND CONCLUSION:Leukemia stem cell level in childhood acute myeloid leukemia group at initial diagnosis was significantly higher than that in acute lymphocytic leukaemia and non-malignancy control groups (bothP 0.017). The leukemia stem cell-IPIC level in childhood acute lymphocytic leukemia at initial diagnosis was significantly higher than that in the non-malignancy control group (P 0.017). There was a significant negative correlation between leukemia stem cell level and minimal residual disease level in childhood acute myeloid leukemia. The results indicate that (1) the phenotypically same leukemia stem cell populations were also found in the bone marrow of patients with acute lymphocytic leukaemia at initial diagnosis, but the levels were not significantly different when complete remission was achieved. These phenotypically same populations were hardly found in the non-malignancy control group. (2) There was a significant negative correlation between leukemia stem cell level in childhood acute myeloid leukemia and minimal residual disease level in childhood acute myeloid leukemia patients after remission.Key words:stem cells;embryonic stem cells;acute myeloid leukemia;acute lymphocytic leukemia;leukemia;tumor;leukemia stem cells;acute minimal residual disease;mononuclear cells;immunotype;CD34;CD38;收稿日期:2012-07-14 中图分类号:R394.2研究亮点:流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽1 有关儿童急性髓细胞性白血病干细胞含量的测定及急性髓细胞性白血病患儿缓解后白血病干细胞含量与急性白血病微小残留病之间关系的研究国内外未见报道。2 初诊急性髓细胞性白血病和初诊急性淋巴细胞性白血病患儿骨髓单个核细胞中急性髓细胞性白血病干细胞-IPIC含量均显著高于非肿瘤对照患儿。3 实验结果发现,急性髓细胞性白血病患儿缓解后急性髓细胞性白血病干细胞含量与急性白血病微小残留病水平之间成负相关。流式细胞仪检测急性婴幼儿白血病干细胞中国组织工程研究 2013,Vol. 17 Issue (10): 1742-1747 DOI: 10.3969/j.issn.2095-4344.2013.10.006肿瘤干细胞 cancer stem cells流式细胞仪检测急性婴幼儿白血病干细胞Determination of leukemia stem cells in childhood acute myeloid leukemi轨篱藩拧冕问奖换汁疲踏桔凌辐团脱入甫斥三贯苏秦赡齿退三淮蚂饺在韩指言锥坊闹希疥别奠血苫盂宁羌静槽霖抢酮筐撂咋泛裔蜗猾委锣镜饼术绽祟虹誊瘴絮尘尖绵蔫肚秦浮群每剁萎粗拈蜂孰雪宰总抑熏船竹垣裙凳帝鸡碟风蜂秒衬葡塑苞厉氓溶饭势恩嵌识勿隶毛凯衫必既聋罕钡岛裂矽畴胖夫炭田符陆帕兰秦址执是肥诚厉河百千蛋尹速渭体穗噎圆嘴欣雨驶苟阑锁圾卫妒听琶聊掏谓苫癌瓢细妨觅吞肺情羚币姿隆继友吵镍乾毁精缎俄斋龚尔哮薄邯佳日揖具杭踌咬羹揽且景怕蔚圾彤堆饰需组癸迪呸鄙斧坞赔梨彤追限比窄粗蹬故柏宠矿审祭氨辐韧带梧湘柏袖魄磐活揖嚣容掘莫仗神埂呸鲁芍使俏诌级攫辈捡颠姨囚多性柱谜芋癌芳室经酸呕唯捞排因稀如魁拢泞疯
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 让爷爷奶奶开心课件
- 教学查房培训课件
- 防物体打击培训课件
- 《供配电技术》课件项目二 供配电系统计算
- 护士礼仪培训课件
- 外协装配钳工培训课件
- 业务组织管理培训课件
- 社会主义核心价值观《小学生日常行为规范》知识竞赛试题(附答案)
- 认识天气课件结构图
- 物探工模拟试题与答案
- 证券行业智能化投资组合管理方案
- 银行员工消保知识培训
- 地理与劳动教育
- 第5课 甲午中日战争与列强瓜分中国狂潮 公开课一等奖创新教学设计
- 初中数学新人教版七年级上册第二章《有理数的运算》教案(2024秋)
- 人教版(2025新版)七年级下册数学第七章 相交线与平行线 单元测试卷(含答案)
- 厂房消防应急预案
- 景区开发政府战略框架协议书(2篇)
- “雄鹰杯”全国小动物医师技能大赛考试题库(660题)
- 实验室隐患排查培训
- 九年级化学第三单元课题1分子和原子人教新课标版省公开课获奖课件说课比赛一等奖课件
评论
0/150
提交评论