中青论坛-周雪(英文)-非高清初稿.ppt

seveofluraneinduedatoptosisanditsmminisminimurerathippampus，2222222222222226，Background，Background，Anan dkj.an 107(1):2-4、后台、KalkmanCJ、etal .Anesthesiology.2009； 110(4):805-12、背景、服务、张力、etal .NeurosciLett.2008； 447(2-3):09-14satoto，m .etal .Anesthesiology，2009.110(3):628-37，minarepreximentsinditathestovfluranecranre hihippcampus (海马组织) isinvsolveinthestorageorconsolidationoflong-termmemries.itslesscausedficitsinspectivealbehaviorofrats .BAC LeverC etal .Nature、2002.416(6876):90-4、neuronalnitricoxidesynthases (nnos ) inthehippcampus nnosplaysanimportantroleinsynapticplasticityandsinvsolveinlearningandmemory.nnosinthehippocampslocalicalzeda tionandmigration .Background、ZhouQG、etal .JNeurochem、2007.103 (5) :84354 nobakihtm、etal .Pathophysiology、2011.18 (4) Scientificquestions :和Spatialmemorydamage？ 长龙- term effect？ Causeapoptosis？ nNOSprotein？histopathologicalexamina-tion、WesternBlotAnalysis、Immunofluorescence、foxsbatterytest、eight-armradialmazetest 12 hafters Postnatalday14(P14 )，Postnatalday28(P28 )，experimental ray 2组3时间点S5 methods，nNOSproteinCaspase-3protein， severoflurane-treatedgroupair-treatedcontrolgroup， researchprogrammme3360 p7pupsexposedto 2.3 % seofluraneorarfor6hrobservationindex : arterialblodgasanalysis，partiseoflurane FengX etal .BrJAnaesth.Apr252012，部分histopathologicalexamination， researchprogrammmme : paraffincoonalsectionsofthehippcampusperformedwithnisslstainingon 12 hafterexposure p 14 andp 28 (n=3each gr observation index : morphologicalandnumbering，12 hafterexposure : nisslstaining parthistopathologicalexamination， 部分histopathologicalexamination，P14:Nisslstaining，部分histopathologicalexamination，P28:Nisslstaining， parthistopathologicalexamination，numberarchangesofthepyramdalneurons，PartWesternblot， researchprogrammme : thesupernatntwasseparatefromhippampuson 12 hafterexposure p 14 and p28 todowesternlotanalyis (n=3each组) observation index : theexprestreationlevelofnnosproteinandccleavedcaspase-3 protein， 第三部分western blot theexprestrationlevelofnosandcleavedcaspase-3 protein，第四部分immuno fluorescence，n=3each组， partfoxsbatterytest thefoxsbatterytestpermitstheassessmentofnormanlandpathologicaldevelopmentoftheyoungratandisndexofthecer researchprogrammme : theneurobehavioraltestswereprformeddailyfromp1top 21 between 08336330 and 1233363000 a.m . correspondingotheratsactivateperiod (n=6each组) observation index 3360，HeulandE，etal .NeurotoxicolTeratol.2010； 32 (4) :5352535253525352535253535353535353535353535353535353535353535353535353535353535353535卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡卡耶伊recor partvieiht-armradilmazetest， thee iht-armradilmazetesthasbeenusedexternevalueatingspatiallearnedmemory whichddependonsonthetructringthetructringth headdiamedarmamezedcandistuishbetweenshort-termworkingrong-termreferencememory .RenWJ，etal . neuropsychopharmacology.2011 36 (5) :979-92 . partvieiht-armradilmazetest researchprogrammme : thee iht-armradilmazetestwasperformeddailyfromp 28 top 39 (p28-p 30 wereinitia observation index : aterverageworkingandrefferenceememoryerrors，partvieiht-armradilmazetest，*P0.05， signicantlydifferentfromcontrol discussion， conclusion earlyexposuretoseofluranecausesong-termneuronallsossandsapotisedecereatio