GFP绿色荧光蛋白

1、1会计学GFP绿色荧光蛋白绿色荧光蛋白l450490nm()GFP峰,但由于长波能量低,细胞忍受能力强,因此更适合于活体检测。,酸或甲醛等。GFP,其作用是任何其它酶类报告蛋白无法比拟的。MethodMinimum cells imageable in vitroMinimum cells imageable in vivoNeed for substrate?Need for anesthesia?Method of visualizationMulticolor imagingGFP11NoNoDirect ImagingYesLuciferase3003000YesYesPhoton c

2、ounting (pseudo-color)No,成过程,但由于标记后肿瘤细胞的荧光信号很易衰减,加上肿瘤细胞分裂,通常23 d 后便难以显示或跟踪经荧光标记过的肿瘤细胞。external images of metastatic tumors.,数个肿瘤细胞形成的肺内微小瘤灶。Orthotopic transplantation of intact tissue Metastatic sites lymph nodes liver lung pancreas spleen diaphragm omentumKU-7-GFP human bladder carcinoma growing on

3、 nude mouse bladder Human U-87-RFP glioma growing in brain of nude mouse. Whole-body image. lRed Fluorescent Protein expression MX-1-GFP and RFP breast carcinoma growing on mammary fat pads of SCID mouse. Whole-body image. Human LOX-1-GFP melanoma metastasized to lung (A), lymp node (B), bone (C) an

4、d adrenal gland (D) of nude mouse. l Non-Small Cell Carcinoma l Adenocarcinoma l Green Fluorescent Protein expression PC-3 human carcinoma growing on prostate and metastasized to numerous lymph nodes of nude mouse. Whole-body image. NUGC-4-GFP human stomach tumor in nude mouse. Primary tumor- red ar

5、row, lymph node metastases - white arrows Skull (a) and rib (b) metastases of PC-3-GFP human prostate carcinoma growing orthotopically in nude mouse. Skeletal metastasis of B16-GFP mouse melanoma in nude mouse Skeletal metastases of H460-GFP human lung cancer orthotopically growing in nude mouse tibia (a), skull (b) and femur ( c )验一致MethodMinimum cells imageable in vitroMinimum cells imageable in vivoNeed for substrate?Need for anesthesia?Method of visualizationMulticolor imagingGFP11NoNoDirect ImagingYesLuciferase3003000YesYesPhoton counting (pseud