常见缓冲溶液的配制

1、常用缓冲溶液的配制方法1 .1.甘氨酸-盐酸缓冲液(0.05mol/L )X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI ,再加水稀释至 200毫升PHXYPHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07 , 0.2 mol/L甘氨酸溶液含15.01克/升2 .邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾+ 0.2 mol/L HCl ,再加水稀释到20毫升pH(20C)XYpH(20C)XY2.254.073.2

2、51.472.4503.4502.653.963.650.992.8503.8503.053.290.59572.640.26232.022邻苯二甲酸氢钾分子量 =204.23 , 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3 .磷酸氢二钠-柠檬酸缓冲液PH0.2 mol/L0.1 mol/L上珏兀公PH0.2 mol/L0.1 mol/L上珏兀公Na2HPO4 丁檬酸Na2HPO4 丁檬酸( 毫( 毫(<#)( 毫升）升）升）2.20.4010.65.210.79.282.41.2405.428.852.62.1818.75.611.18.402.83.1765.857.91

3、3.04.1117.86.011.67.373.24.9426.206.783.45.7016.86.412.06.153.66.4436.695.453.87.1015.86.812.64.554.07.7197.033.534.28.2815.07.213.22.614.48.8267.421.834.69.3514.37.613.81.274.89.8607.850.855.010.313.58.014.50.5506512.9012.2911.7211.1810.6510.149.7015.4516.4717.3918.1718.7319.1519.45NaHPM子量=14.98 ,

4、0.2 mol/L 溶液为 28.40 克/升。NaHPQ2H2O分子量=178.05 , 0.2 mol/L 溶液含 35.01 克/升GH2O HO分子量=210.14 , 0.1 mol/L 溶液为 21.01 克/升。4 .柠檬酸-氢氧化钠-盐酸缓冲液PH钠离子浓度（mol/L ）柠檬酸（克）C6H8O7 H2O氢氧化钠（克）NaOH97%盐酸（毫升）HCl（浓）最终体积（开）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50

5、.3826615612610使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。p0.10.1mol/L0.10.1mol/Lp0.10.1mol/LH柠檬酸柠檬酸钠H柠檬酸318.61.458.2.017.22.8.07.3316.04.056.4.214.95.1.25.5314.06.054.7.413.16.9.43.8312.37.752.8.611.48.6.62.0310.39.751.4.89.210.8.85.柠檬酸-柠檬酸钠缓冲液(0.1 mol/L )0.46.0.046.2.246.4.446.6.64.8柠檬酸C6H8O

6、7 H2Q 分子量210.14, 0.1 mol/L溶液为21.01克/升。柠檬酸钠 Na C6Ho-2HO 分子量294.12, 0.1 mol/L 溶液为29.41克/毫升。6.乙酸-乙酸钠缓冲液(0.2 mol/L )0.20.2mol/L0.30.3mol/L0.20.2pH(18NaAcHAcpH(18NaAcC)(<#)(<#)C)(<#)2.60.759.254.85.903.81.208.805.07.004.01.808.205.27.904.22.657.355.48.604.43.706.305.69.104.64.905.105.89.40NaAc 3

7、HbO分子量=136.09 , 0.2 mol/L 溶液为 27.22 克/升7 .磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)0.20.2mol/L0.30.3mol/L0.20.2mol/L0.p NazHPONaHzPOp NaHPO卜H(<#)(<#)H(<#)58.092.0761.0.810.090.0.067.0512.387.7772.0.915.085.0.177.0618.581.5781.0.022.577.5.284.0626.573.5787.0.131.568.5.389.5637.562.5791.5.243.556.5.493.06

8、49.551.0794.7.355.045.0.567.4.667.56.77.66.87.76.98.86.0.9NaHPO 2HO分子量=178.05, 0.2 mol/L 溶液为 85.61 克/升NaHPO 2Ho分子量=358.22 , 0.2 mol/L 溶液为 71.64 克/升NaHPO 2Ho分子量=156.03, 0.2 mol/L 溶液为 31.21 克/升(2)磷酸氢二钠-磷酸二氢钾缓冲液(1/15 mol/L )Pmol/L/15Na2HPOmol/L/15KH 2POP mol/L/15Na 2HPOmol/L/15KH 2P(H(<#)(毫升)H(<#

9、)(<#)40.109.9077.003.00.920.509.50.178.002.0051.009.0079.001.00.292.008.00.389.500.5053.007.0079.750.25.914.006.00.739.900.1065.005.00810.000.246.004.00.0468.47.3468.64.6768.81.186.98NaHPO 2HO分子量=178.05 , 1/15M溶液为 11.876 克/升KHPO分子量=136.09 , 1/15M溶液为9.078克/升。8 .磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO+ Y毫

10、升0.2N NaOH加水稀释至29毫升X (毫Y (毫pH(20X (毫Y (毫pH(20C)升）升）C)升）升）5.850.377.052.966.0527.2536.250.577.453.506.4507.6506.650.867.853.956.8508.0501.264.28001.784.5202.36504.6809 .巴比妥钠-盐酸缓冲液（18C）PH0.04M巴比妥钠溶液0.2V盐酸PH0.04M 巴比妥钠溶液（毫升）0.2N盐酸(<#)6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.41001

11、5.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10 . Tris -盐酸缓冲液（0.05M, 25C）50毫升0.1M三羟甲基氨基甲烷（Tris ）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升PHX（文）PHX（文）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.601

12、2.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris ） HOCH2 CH2OHC HOCH2 NH2 分子量=121.14;0. 0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严C11 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M 硼砂（毫升）0.2M硼砂(<#)PH0.05M 硼砂（毫升）0.2M硼酸(<#)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02

13、.0硼砂 NaBO H2O,分子量=381.43;0.05M 溶液（=0.2M硼酸根）含 19.07 克/硼酸HBO,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12 .硼酸-硼砂缓冲液（0.2M硼酸根）PH0.05M 硼0.2M硼酸PH0.05M 硼0.2M硼酸砂（毫升）(<#)砂（毫升）(<#)7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO 10H2O,分子量=381.43;0.05M 溶液（=0.2M硼酸根）含 19.0

14、7 克/ 升。硼酸HBO,分子量=61.84, 0.2M 溶液为12.37克/升。硼砂 易失去结晶水，必须在带塞的瓶中保存。13 .甘氨酸-氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升PHXYPHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升14 .硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOHb口水稀释至200毫升PHXYPHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂NaBO 10H2O,分子量=381.43;0.05M溶液为19.07克/升15 .碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg+存在时不得使用PH0.1MNaCO（毫0.1MNHCQ 毫20 c37 c升）升）9.168.77199.409.12289.519.