常用缓冲溶液的配制方法

1、常用缓冲溶液的配制方法1.甘氨酸-盐酸缓冲液(0.05mol/L)X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至 200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量 =75.07，0.2 mol/L甘氨酸溶液含15.01克/升。2.邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl，再加水稀释到 20毫升pH(20 C )XYpH(20 C)XY2.254.0703.25

2、1.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23, 0.2 mol/L邻苯二甲酸氢溶液含40.85克/升3.磷酸氢二钠-柠檬酸缓冲液pH0.2 mol/LNa2HPO4(毫升)0.1mol/L柠檬酸(毫升)pH0.2mol/LNa2HPO4(毫升)0.1 mol/L柠檬酸(毫升)2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.0

3、4.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量 =14.98 , 0.2 mol/L 溶液为 28.40

4、 克/升。Na2HPO4 2H2O 分子量 =178.05, 0.2 mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量 =210.14, 0.1 mol/L 溶液为 21.01 克/升。4 柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度 mol/L柠檬酸克C6H 8O7 H 2O氢氧化钠克NaOH 97%盐酸毫升HCl 浓最终体积刑2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时可

5、以每升中参加 1克克酚，假设最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5 柠檬酸-柠檬酸钠缓冲液0.1 mol/L pH0.1 mol/L 柠檬酸毫升0.1 mol/L柠檬酸钠毫升pH0.1 mol/L柠檬酸 毫升0.1 mol/L柠檬酸钠毫升3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610

6、.39.76.61.418.64.89.210.8柠檬酸C6H8O7 出0:分子量210.14, 0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O7 2H2O:分子量294.12, 0.1 mol/L溶液为29.41克/毫升。6 .乙酸-乙酸钠缓冲液0.2 mol/L pH18 C 0.2 mol/LNaAc毫升0.3 mol/LHAc毫升pH18 C0.2 mol/LNaAc毫升0.3 mol/LHAc毫升2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.60

7、1.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac 3H2O 分子量 =136.09, 0.2 mol/L 溶液为 27.22 克/升。7 磷酸盐缓冲液1磷酸氢二钠-磷酸二氢钠缓冲液0.20.2 mol/L0.3 mol/L0.2 mol/L0.3 mol/LpHNa2HPO4 毫升NaH 2PO4毫升pHNa2HPO4毫升NaH2PO4毫升5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.

8、481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO4 2H2O 分子量 =178.05, 0.2 mol/L 溶液为 85.61 克/升。Na2HPO4 12H2O 分子量 =358.22 , 0.2 mol/L 溶液为 71.64 克/升。Na2HPO4 2H2O 分子量 =156.03, 0.2 mol/L 溶液为 31.21 克/升

9、。2磷酸氢二钠-磷酸二氢钾缓冲液1/15 mol/L pHmol/L/15Na 2HPO4毫升mol/L/15KH 2PO4 毫升pHmol/L/15Na 2HPO4毫升mol/L/15KH 2PO4 毫升4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO4 2H2O 分子量 =1

10、78.05, 1/15M 溶液为 11.876 克/升。KH2PO4分子量 =136.09, 1/15M 溶液为 9.078 克/升。&磷酸二氢钾-氢氧化钠缓冲液0.05MX毫升0.2M K 2PO4 + Y毫升0.2N NaOH加水稀释至29毫升pH20 CX 毫升Y 毫升pH20 CX 毫升Y 毫升5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液18CpH0.04M巴比妥0.2V盐酸pH0.0

11、4M 巴比0.2N盐酸毫钠溶液妥钠溶液毫升升6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10. Tris -盐酸缓冲液0.05M , 25C50毫升0.1M三羟甲基氨基甲烷Tris溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。pHX毫升pHX毫升7.1045.78.

12、1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷TrisHOCH2 CH2OH CHOCH2 NH2分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11硼酸-硼砂缓冲液0.2M硼酸根pH0.05M硼砂毫升0.2M 硼砂毫升pH0.05M硼砂毫升0.2M 硼酸毫升7.41.09.08.23

13、.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O7H2O,分子量=381.43;0.05M溶液=0.2M硼酸根含19.07克/升。硼酸H2BO3，分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11硼酸-硼砂缓冲液0.2M硼酸根pH0.05M硼砂0.2M 硼酸pH0.05M硼砂0.2M 硼酸毫升毫升毫升毫升7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂 Na2B4O

14、7 IOH2O,分子量=381.43;0.05M 溶液=0.2M 硼酸根含 19.07 克 /升。硼酸H2BO3,分子量=61.84, 0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。12甘氨酸-氢氧化钠缓冲液0.05MX毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13硼砂-氢氧化钠缓冲液0.05M硼酸根X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂 Na2B4O7 10H20,分子量=381.43;0.05M 溶液为 19.07 克/升。14.碳酸钠-碳酸氢钠缓冲液0.1MCa2+、Mg2+存在时不得使用pH0.1MNa2CO3毫升0.1MN 2HCO 3毫升20C37 C9.168.77199.409.12289.519.40379.789.