淡水海水培养基配方

1、SE MediumWorking solution (g/l)NaNO30.25gK2HPO4 . 3H2O0.075gMgSO4 . 7H2O0.075gCaCl2 . 2H2O0.025gKH2PO40.175gNaCl 0.025Soil extract *40mlFeCl3·6H2O0.005FeEDTA1mlA5 solution *1distilled water958Soil Extract(土壤提取液)配置方法取花园土未施过肥0。5kg置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热2小时，冷却数小时，在无菌条件下过滤，取上清液，将灭菌蒸

2、馏水加入上清液至总体积1000毫升。土壤提取液保存在4ºC备用。* Composition of the A5 solution Add to 100 ml of distilled water:H3BO3286 mgMnCl2 . 4H2O181 mgZnSO4 . 7H2O22 mgCuSO4 . 5 H2O7.9 mg(NH4)6Mo7O24 . 4H2O3.9 mgEDTA-Fe的配置方法：将EDTA和FeCl3·6H2O分别溶于水和HCl(0.1N),混匀即可。Na2EDTA1gdistilled water50mlFeCl3·6H2O81 mgHCl（

3、0.1N）50mlf/2 Medium (Guillard & Ryther 1962, Guillard 1975)To 950 mL filtered seawater add:QuantityCompound Stock SolutionMolar Concentration in Final Medium1 mL NaNO3 75 g/L dH2O 8.83 x 10-4 M1 mL NaH2PO4 · H2O 5 g/L dH2O3.63 x 10-5 M1 mL *Na2SiO3 · 9H2O* 30 g/L dH2O* 1.07 x 10-4 M*1

4、mL f/2 trace metal solution (see recipe below)-0.5 mLf/2 vitamin solution (see recipe below)-Make final volume up to 1 L with filtered seawater and autoclave. *Note: Autoclaved f/2 medium produces extensive silica precipitate. We delete silicate when it is not required by the alga (see f/2-Si medium

5、 below).  f/2 Trace Metal Solution(Guillard & Ryther 1962, Guillard 1975) To 950 mL dH2O add:QuantityCompound Stock SolutionMolar Concentration in Final Medium3.15 g FeCl3 · 6H2O -1 x 10-5 M4.36 g Na2EDTA · 2H2O -1 x 10-5 M1 mL CuSO4 · 5H2O 9.8 g/L dH2O4 x 10-8 M1 mL Na2MoO4 · 2H2O 6.3 g/L dH2O 3 x 10-8 M1 mL ZnSO4 · 7H2O 22.0 g/L dH2O 8 x 10-8 M1 mL CoCl2 · 6H2O 10.0 g/L dH2O 5 x 10-8 M1 mL MnCl2 ·