苦荞壳提取物对大鼠血脂的调节作用

1、苦荞壳提取物对大鼠血脂的调节作用         07-08-17 17:38:00     编辑：studa20                              

2、60;                        作者：童红莉，田亚平，汪德清，董振南，邓心新【关键词】  苦荞Role of tartarian buckwheat shell extract  in regulation of blood lipid in rats with hyperlipidemia【Abstract】 AIM: To

3、 investigate the antihyperlipidemia effect of tartarian buckwheat shell extract (TBSE) in rats and its mechanism. METHODS:  Fifty male Wistar rats were randomly  separated into 5 groups according to body weight. They were fed respectively with normal diet, high lipid diet, high lipid diet

4、supplemented with different concentrations of TBSE (0.825, 0.165 g/kg), high lipid diet supplemented with Lipingzhi capsules (LPZ, 0.067 g/kg). After 12 weeks, the concentrations of serum Ch, Tg, HDLC, LDLC, MDA, the activity of SOD and GPX, liver index were measured. RESULTS:  Compared with th

5、ose in normal diet group, the serum Ch, Tg, LDLC, MDA and liver index in high lipid diet group increased significantly (P<0.05), the serum HDLC and SOD decreased significantly (P<0.05). The serum GPX activity decreased and the body weight increased, but there was no significant statistical dif

6、ference (P>0.05). In LPZ group, compared with those in high lipid diet group, the serum Ch, Tg, LDLC and MDA decreased significantly (P<0.05), the serum GPX activity increased significantly (P<0.05), but the serum HDLC, SOD, body weight and liver index had no significant statistical changes

7、 (P>0.05). Compared with those in high lipid diet group, the serum Ch, Tg, LDLC, MDA and liver index in TBSE group decreased significantly (P<0.05), the serum GPX and SOD activity increased significantly (P<0.05). The serum HDLC increased and the body weight decreased but there was  no

8、 significant statistical difference (P>0.05). CONCLUSION:   TBSE can reduce the blood lipid and liver index and enhance the blood antioxidant activity in rats with hyperlipidemia, which can protect the normal tissues against the injury induced by high lipid diet.【Keywords】 tartarian buc

9、kwheat shell extract; hyperlipidemia; disease  models, animal;  rats;  blood lipid regulation; antioxidants【摘要】 目的： 观察苦荞壳提取物对高脂血症大鼠血脂的调节作用，探讨其机制. 方法： 将雄性Wistar大鼠50只按体质量随机分为普通饲料组、高脂模型组、苦荞壳提取物高剂量组(0.825 g/kg)、低剂量组(0.165 g/kg)和力平之对照组(0.067 g/kg). 于实验后第12周测定大鼠血清Ch, Tg, HDLC, LDLC, MDA含量,

10、SOD, GPX活力和肝指数，并观察大鼠肝组织病理变化. 结果： 高脂模型组大鼠血清Ch, Tg,  LDLC, MDA含量和肝指数显著高于普通饲料组(P<0.05); HDLC, SOD显著低于普通饲料组(P<0.05); GPX低于普通饲料组，体质量高于普通饲料组，但无统计学差异(P>0.05). 力平之组大鼠血清Ch, Tg,  LDLC, MDA含量显著低于高脂模型组(P<0.05)；GPX显著高于高脂模型组(P<0.05); HDLC, SOD, 肝指数和体质量与高脂模型组之间无统计学差异(P>0.05). 苦荞壳提取物各组大鼠

11、血清Ch, Tg, LDLC, MDA和肝指数显著低于高脂模型组(P<0.05); SOD, GPX显著高于高脂模型组(P<0.05); HDLC高于高脂模型组，体质量低于高脂模型组，但无统计学差异(P>0.05). 结论： 苦荞壳提取物可降低实验性高血脂血症大鼠的血脂和肝指数，提高血液的抗氧化能力，减轻高脂饮食导致的氧化损伤.【关键词】 苦荞麦壳提取物；高脂血症；疾病模型，动物；大鼠；血脂调节；抗氧化剂0引言苦荞麦是一种药食两用植物，祖国医学本草纲目记载：苦荞麦性味苦、平、寒，有益气力、续精神、利耳目、降气宽肠健胃的作用1. 现代临床医学观察表明，苦荞麦粉及其制品具有降血糖

12、、血脂，增强人体免疫力的作用. 这些作用都与苦荞麦中含有的营养成分有关. 据报道，苦荞麦中含有丰富的黄酮类物质，其主要成分为芦丁2. 此外苦荞杆、叶、花等含有的黄酮类物质也有降血糖、血脂的作用3,4. 但苦荞壳提取物的相关研究较少. 我们观察了苦荞壳提取物对实验性高脂血症大鼠的血脂调节作用，并探讨了其机制.1材料和方法1.1材料苦荞壳提取物由长城制药厂制备，芦丁含量为40 g/kg提取物. 血清总胆固醇试剂盒（Ch）、甘油三酯(Tg)试剂盒购自柏定生物工程有限公司. 高、低密度脂蛋白胆固醇（HDLC，LDLC）试剂盒购自利德曼生化技术有限公司. 谷胱甘肽过氧化物酶（GPX）试剂盒购自九强生物工

13、程有限公司. 脂质过氧化物（MDA）、超氧化物歧化酶（SOD）试剂为本实验室配制，黄嘌呤氧化酶购自Sigma公司. 雄性Wistar大鼠50只，体质量（257±15） g，由解放军总医院实验动物中心提供. 1.2方法健康雄性Wistar大鼠50只，按体质量随机分为5组，每组10只. 普通饲料组，喂普通饲料. 高脂模型组，喂高脂饲料(主要成分为: 普通饲料78.8%，猪油10.0%，蛋黄粉10.0%，胆固醇1.0%和胆酸盐0.2%). 苦荞壳提取物高、低剂量组，分别将苦荞壳提取物以0.825, 0.165 g/kg的剂量加入高脂饲料. 力平之组，将力平之以0.067 g/kg 的剂量加入高脂饲料. 所有饲料均委托北京科澳协力饲料有限公司加工.