红茶提取物茶多酚

1、红茶提取物茶多酚产品规格： 30%,60% 茶多酚检测方法： Folin D法有效成分： 茶多酚产品来源： 山茶科植物山茶的叶提取方法： 乙醇提取产品外观： 棕黄色粉末1. 理化性质分子结构类型：一类多酚化合物溶解性：易溶于水及乙醇，味苦涩2. 质量标准检验2.1 鉴别：（对照品：没食子酸）2.1.1 TLC检查，与对照品Rf值、颜色一致2.1.2 IR检查，与对照品指纹图谱吻合（红外吸收光谱）2.2 红茶茶多酚的检测方法：2.2.1 原理福林酚试剂氧化茶多酚中-OH基团并显蓝色，最大吸收波长为765nm， 用没食子酸作校正标准定量茶多酚。2.2.2 试剂及设备1. 色谱纯乙腈2. 甲醇1.

2、甲醇水溶液（体积比）=7:32. 碳酸钠(Na2CO3)3. 福林酚试剂4. 10%福林酚试剂（现配）：将20mL福林酚试剂转移到200mL容量瓶中，用水定容并摇匀。5. 7.5% Na2CO3溶液（质量浓度）：称取37.50g0.01gNa2CO3（10.3），加适量水溶解，转移至500mL容量瓶中，定容至刻度，摇匀。6. 没食子酸标准储备溶液(1000g/mL): 称取0.110g0.001g没食子酸(GA,相对分子质量188.14)，于100mL容量瓶中溶解并定容至刻度，摇匀.7. 没食子酸工作液：用移液管分别移取1.0，2.0，3.0，4.0，5.0mL的没食子酸标准储备溶液(10.8

3、)于100mL容量瓶中，分别用水定容至刻度，摇匀，浓度分别为10，20，30，40，50g/mL。2.2.3 样品液配置准确称取10g红茶提取物样品，用适量水溶解，转入100mL容量瓶中，加水定容至刻度，摇匀，待测。 2.2.4 样品含量的检测用移液管分别取没食子酸工作液、水及测试液各1.0mL于刻度试管内，在每个试管内分别加入5.0mL的10%福林酚试剂，摇匀。反应3min8min内，加入4.0mL7.5% Na2CO3溶液，加水定容至刻度、摇匀。室温下放置60min。用10mm比色皿、在765nm波长条件下用分光光度计测定吸光度（A）。根据没食子酸工作液的吸光度（A）与各工作溶液的没食子酸

4、浓度，制作标准曲线。 2.2.5 结果计算比较试样和标准工作液的吸光度，按下式计算：式中：A样品测试液吸光度；V样品液体积；d稀释因子(稀释倍数)；SLOPEstd 没食子酸标准曲线的斜率；m样品干物质含量，%；m1样品质量，单位为克(g)。2.3 检查：干燥失重： 2%灰分： 0.5%重金属： 百万分之五农药残留： 百万分之二溶剂残留：0.1%2.4 卫生检查细菌总数：1000/g霉菌总数：100/g大肠杆菌： 无沙门菌： 无2.5 粒度： 均匀接近细微颗粒2.6 包装： 双层朔料袋封口、外包装纸桶、桶外印有品名、毛重、批号、生产单位、生产时间、有效时间、贮运方法等。2.7 有效期： 2年2

5、.8 贮藏： 阴凉、干燥、避光贮放3. 提取工艺流程图红茶提取物茶多酚的生产工艺红茶原料清水浸泡乙醇提取残渣 提取液 过滤 浓缩 质量分析和评价 高压高温瞬时灭菌 喷雾干燥 筛分 产品包装 茶多酚4. 描述基本信息：茶多酚又称茶鞣质或茶单宁，是茶叶中多酚类物质的总称，也是形成茶叶色香味及起保健功效的主要成分之一。由于多酚结构的存在，茶多酚易氧化，可用于清除体内过剩的自由基、阻止脂质过氧化，提高机体免疫力，延缓衰老等方面。茶多酚等活性物质具解毒和抗辐射作用，能有效地阻止放射性物质侵入骨髓，并可使锶90和钴60迅速排出体外，被健康及医学界誉为“辐射克星”。 喝茶保健养生已经成为世界性的健康理念。茶

6、多酚的主要功效： 1 具有很强的消除有害自由基的作用。2 抗衰老作用。3 抗辐射作用。4 对癌细胞的抑制作用。5 抗菌、杀菌作用。6 对艾滋病病毒的抑制作用。5. 产品导向：美容、减肥、保健品、药品、保鲜剂6. 安全性及最小用量: 安全Black Tea Extract PolyphenolsProduct Specification: 30%,60% PolyphenolsTest method: Folin DChemical Name: PolyphenolsRaw material: Leaf of Camellia sinensis O. Ktze.Extract Method: G

7、rain AlcoholProduct Type: Brown yellow powder1. Physical and Chemical PropertiesMolecular structure of type: a series Polyphenol compoundsSolubility: Easy dissolve in water and ethanol.2. Quality standards testing2.1 Identification: (reference substance: Gallic acid )2.1.2 TLC examination: reference

8、 substance Rf value and color are consistency2.1.3 IR inspection: in line with the reference substance fingerprint (infrared absorption spectra)2.2 Folin-Ciocalteu method to test Ployphenol in Black Tea Extract2.2.1 TheoryFolin-Ciocalteu solvent can oxidant the OH group in ployphenol and show blue c

9、olor, the m is 765nm; to measure the concentration of ployphenol by using Gallic acid as calibrations.2.2.2 Reagents and equipment1. Chromatography grade Acetonitrile 2. Methanol3. Methanol-Water = 7:3 solvent4. Na2CO3 solvent5. Folin-Ciocalteu solvent6. 10% Folin-Ciocalteu (fresh): 20mL Folin-Cioca

10、lteu solvent be transfer to 200mL volumetric flask, and mix with water to volume.7. 7.5% Na2CO3(weight) solution: accurately takeing 37.50g0.01gNa2CO3 dissolve in water, then transfer to 500mL volumetric flask, and mix with water to volume.8. Gallic acid standard store solution: accurately takeing 0

11、.110g0.001g Gallic acid( GA, MW:188.14) into 100mL volumetric flask, and mix with water to volume.9. Gallic acid working solution: accurately transfer 1.0, 2.0，3.0，4.0，5.0mL Gallic acid standard store solution into 100mL volumetric flask, and mix with water to volume. The concentration series are 10

12、, 20, 30, 40, 50g/mL.2.2.3 Preparation for test sampleAccurately taking 10g of Black Tea Extract samples dissolved in appropriate water, then transfer into 100mL volumetric flask, adding water to volume.2.2.4 Sample content testPartly taking 1.0mL Gallic acid working solution, Water, Test sample add

13、 into 3 graduated test tube. Each test tube be add into 5.0mL 10% Folin-Ciocalteu solvent, mixed. Let the liquid reaction 3min8min, then add 4.0mL 7.5% Na2CO3 solution and water to volume, mixed. Lay at room temperature. Detect the absorbance(A) by a 10mm cuvette at 765nm wave.Making the working cur

14、ve according to the absorbance and the concentration of the Gallic acid.2.2.5 Calculate According to the absorbance(A), calculate the ployphenol as follow formula:Label: Athe absorbance of the test sampleVthe volume of the test sampleddilution factorSLOPEstd the slope of the Gallic acid working curv

15、emweight of dried sample(%)m1weight of sample(g)2.3 ExamineLoss on Drying: NMT 2%Ash: NMT 0.5%Heavy Metals: NMT 5 ppmPesticide Residues: NMT 2 ppmSolvents Residues: NMT 0.1%2.4 Hygiene inspectionTotal bacteria: NMT 1000/gTotal Streptomyces: NMT 100/gE.Coli: NoneSalmonella: None2.5 Sieve Analysis: 10

16、0% pass 80mesh2.6 Packing: Packed in paper-drums and two plastic-bags inside. Barrels outside printed name, gross weight, batch number, production units, production time, effective time, storage and transportation methods.2.7 Effective time: two years Storage situation: Stored in a cool and dry well

17、-closed container, keep away from moisture and strong light / heat.3. Extraction Process Flow DiagramManufacturing Process of Black Tea Extract Ployphenol Black TeaSoak with waterExtract with alcohol Residue Extract solution Filter the extract solution Concentration Quality analysis and evaluation S

18、terilization using high pressure and high temperature for 510 Second Spray drying Sift the powdered extract Packing the powder Ployphenol4. Basic information: Tea health has become a worldwide health concepts. Tea polyphenols, also call tea tannin, is a whole name of a series polyphenols in tea; it is also the main ingredients in tea which shape the special flavour of smell, taste and Color of Solution, also is the main ingredients with health care activity. Due to the polyphenol structure, tea ployphenol is