纳米细菌肾结石模型血、尿生化的动态观测

1、纳米细菌肾结石模型血、尿生化的动态观测纳米细菌肾结石模型血、尿生化的动态观测1959202111-0058-06Abstract：Objective To construct a kidney model of nano-bacteria Wistar male rats， and to dynamically monitor blood and urine biochemical indicators， in order to further study the role of nanobacteria in the development of kidney stones， provide

2、 data support， and then the etiology of kidney stones. The next step is to explore new ideas. Methods 60 rats were randomly divided into a nanobacteria group and a blank control group， with 30 rats in each group. The nanobacterial group was given a 1.2 ml suspension of NB in the tail vein， and the b

3、lank control group was given a 1.2 ml saline solution in the tail vein. The experimental period was 10 weeks. Three rats died in each group per week. Blood and urine were collected before sacrifice. Specimens for biochemical analysis， and take fresh kidney specimens on both sides for pathological cr

4、ystallization and calculation of renal to body ratio. Results Since the 8th week， the renal body ratio of the nanobacterial group was larger than that of the blank control group，the difference was statistically significant P0.05. By the end of the 10th week， there were 11 kidney crystals in the nano

5、-bacterial group， and the blank control group was negative. The positive rate of renal crystal in the nano-bacterial group was higher than that in the blank control group，the difference was statistically significant P0.05.Compared with the blank control group， the serum creatinine of the nanobacteri

6、al group was 4 to 8 weeks.At the 3rd to 8th week， blood uric acid and urea nitrogen and urinary calcium increased at the 3rd to 9th week. At the 8th week， the urine pH of the nanobacterial group was lower than that of the blank control group. The blood calcium level of the nanobacterial group was hi

7、gher than that of the 6th to 7th week. The blank control group had significant differences P0.05. Conclusion The effective increase of renal body ratio， crystallization rate， serum creatinine， blood uric acid， blood urea， urinary calcium and other important indicators have shown the successful preparation of the experimental model of kidney calculus in nano-bacteria Wistar male rats， which further reveals NB. The role played by the formation of stones and the subsequent further study of the etiology of kidney stones provide a good experimental basis and data support. 0.05。纳米细菌组血磷与血镁