北大神经生物学2绪论PPT学习教案

1、会计学1北大神经生物学北大神经生物学2绪论绪论Day of treatment1234567891060708090100110120130Control2 Hz2/100 Hz100 Hzn=13-16*Heart Rate (beat/min)第1页/共36页Body Weight (kg)01234567891011Days of Treatment45505560Control2Hz2/100Hz100Hz(n=26-32)Effect of HANS on Body Weight during Opiate Withdrawal*第2页/共36页第3页/共36页CRAVING: th

2、e most difficult issue一朝吸毒，终身想毒。第4页/共36页第5页/共36页The auto-track conditioned place preference (CPP) instrument第6页/共36页Morphine-induced conditioned place preference (CPP)Dose-dependent effect00.01 0.03 0.10.31.03.0 10.00.000.250.500.75* *n=9-12Mor ( mg/kg )*p0.05, *p0.01, *p 100 Hz100 Hz 2 Hz第13页/共36页E

3、lectroacupuncture treatment for drug addictionVTAOpiateGABANAcDADATCocaineEuphoriaEnkDynElectroacupuncture2 Hz100 HzPresynaptic inhibitionSubstitution第14页/共36页A100 bp2000 bp1000 bp750 bp500 bp250 bpDL 2000 NSCPP2 Hz100 HzrestrainGAPDH (532 bp)PPD (250 bp)Effect of 100Hz EA on the level of PPD mRNA i

4、n NAc of CPP rats B0.00.51.01.5Grey scale ratios(PPD/GAPDH)Treatment*#n=4NSCPP2 Hz100 Hzrestrain第15页/共36页Effect of 2Hz EA on the level of PPE mRNA in NAc of CPP rats Grey scale ratios(PPE/GAPDH)0.000.250.500.751.00NSCPP*#2 Hz100 Hzrestrainn=42000 bp1000 bp750 bp500 bp250 bp100 bpDL2000CPP2 Hz100 Hzr

5、estrainGAPDH（532 bp）PPE（402 bp）NS第16页/共36页美国权威性美国权威性“物质滥用物质滥用”教科书教科书Joyce Lowinson 等主编等主编第第4版，版，2004“针刺戒毒针刺戒毒”一章一章由韩济生编写由韩济生编写第17页/共36页肥肥 胖胖 症症能量代谢失衡能量代谢失衡对美食的渴求对美食的渴求药物成瘾药物成瘾身体依赖：戒断症状身体依赖：戒断症状精神依赖：心瘾精神依赖：心瘾痛与镇痛痛与镇痛感知功能感知功能情绪反应情绪反应第18页/共36页肥胖的发展趋势肥胖的发展趋势第19页/共36页肥胖发病机理肥胖发病机理GenesEnvironmental factor

6、s 第20页/共36页048121620242832-4-3-2-10 A HANS B C HANS Dn=16Time ( week )ABCD-4-3-2-10*HANS ( 3 time / week )Change of body weight ( kg )梁门，一对。天枢，一对。水道，一对。梁丘，公孙。频率：2/100 Hz每周3次每次30 分钟第21页/共36页02468101214160100200300400500600700High energy diet (n =80)Normal diet (n =20)*Time (week)Body weight ( g )Cont

7、rolDRDIO0100200300400500600700800*#High energy dietBody weight ( g )* P0.001 vs. control and DR; # P0.05 vs. Control第22页/共36页促进摄食和抑制摄食的神经肽促进摄食 feedingNPY, Orexin,AGRP, MCH抑制摄食 feedingLeptin, CART, a-MSH, CRHNPYOrexinAGRPMCHCARTLeptin -MSH Insulin瘦胖ThinObese第23页/共36页100Hz036912151821242730Time ( day

8、)9092949698100102104106108110Body weight ( % )DIO大鼠实验结果（体重大鼠实验结果（体重%）Restrain2Hz第24页/共36页* P0.001 vs. DIO; # p0.01, # P0.001 vs. Restriction-30-20-100102030405060*# Body weight ( g )DIORestrain100 Hz2 Hz第25页/共36页大鼠实验结果（大鼠实验结果（ACTHACTH）Normal DR DIO Restrain 100Hz EA 2Hz EA051015202530*High energy di

9、et*Plasma ACTH (ng / ml)* P0.05 vs. Normal, DR and DIO.第26页/共36页NormalDRDIORestriction100Hz EA2Hz EA020406080100120140160High energy diet*#Energy intake ( kcal / day )* P0.001 vs. other groups; # P0.001 vs. 2Hz and 100 Hz EA group 第27页/共36页 CART -MSH第28页/共36页ControlDiet induced ObesityDiet Resistant

10、Restrain100Hz2HzCART immunoreactivity in hypothalamus第29页/共36页ControlDiet induced ObesityDiet ResistantRestrain100 Hz 2Hz-MSH immunoreactivity in hypothalamus第30页/共36页 n = 5NormalDRDIORestrain100Hz EA0255075100125 *High energy diet CART positive cells in ARCHigh energy dietNormalDRDIORestrain100Hz EA2Hz EA010203040506070* a-MSH positive cells in ARC *2Hz EACART-MSH第31页/共36页 国际神经科学神经科学领域：英国TiNS杂志（2003） 国际针灸针灸领域：德国SEIRIN针灸奖（2002） 国际戒毒戒毒理论：美国“物质成瘾”经典教科书 临床戒毒戒毒实践：韩氏戒毒一年以上不复吸20例36年针灸研究，12年戒毒研究所获成绩第32页/共36页Trends in Neuroscience, 2003; 26: 17-22 第33