澳洲茄边碱作用机制 - Medchemexpress - MCE中国

1、Product Data SheetSolamargineCat. No.: HY-N0069CAS No.: 20311-51-7分式: CHNO分量: 868.06作靶点: P-glycoprotein; Apoptosis作通路: Membrane Transporter/Ion Channel; Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (115.20 mM; Need ultrasonic)SolventMass1

2、mg 5 mg 10 mgConcentration制备储备液1 mM 1.1520 mL 5.7600 mL 11.5199 mL5 mM 0.2304 mL 1.1520 mL 2.3040 mL10 mM 0.1152 mL 0.5760 mL 1.1520 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先

3、按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (2.88 mM); Clear solution此案可获得 2.5 mg/mL (2.88 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100

4、 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (2.88 mM); Clear solution此案可获得 2.5 mg/mL (2.88 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄均匀。DMSO 储备液加到

5、 900 L 20% 的 SBE-CD 理盐溶液中，混合BIOLOGICAL ACTIVITY物活性 Solamargine 种来 于茄属植物的类 醇 Solasodine 的衍物，在多种癌症中均表现出抗癌活性。Solamargine 诱导选择性细胞毒性和 P-gp 抑制作。Solamargine 通过下调 MMP-2 和 MMP-9 的表达和活性来显着抑制 HepG2 细胞的迁移和侵袭。体外研究 Solamargine (15 g/ml; 72 hours) predominantly stimulates the cells of the G2/M phase to apoptosis3.

6、Solamargine (72 hours) dramatically inhibits the proliferation of SMMC-7721 and HepG2 cells in a dose- and time-dependent manner. The IC50s are 9.21 and 19.88 g/ml, respectively3.Cell Viability Assay3Cell Line: SMMC-7721cellsConcentration: 15 g/mlIncubation Time: 72 hoursResult: Displayed a signific

7、ant increase of sub-G1.体内研究 Solamargine (10 mg/kg; intragastric administration; once daily for 8 days) exhibits an antitumor effect and promotesthe apoptosis of GC in vivo4.Animal Model: Female specific pathogen-free BALB/c nude mice weighing 18-20 g (6-8-weeks-old)4Dosage: 10 mg/kgAdministration: I

8、ntragastric administration; once daily for 8 daysResult: Tumor growth was significantly inhibited .户使本产品发表的科研献 Cancers (Basel). 2019 Mar 12;11(3). pii: E353. Food Chem. 2019 Dec. J Food Drug Anal. 2018 Apr;26(2):751-760. Int J Oncol. 2019 Mar 12. Int J Oncol. 2019 Mar;54(3):905-915.See more customer

9、 validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Burger T, et al. Solamargine, a bioactive steroidal alkaloid isolated from Solanum aculeastrum induces non-selective cytotoxicity and P-glycoproteinPage 2 of 3 www.MedChemEinhibition. BMC Complement Altern Med. 2018 May 2;18(1):137.2. S

10、ani IK, et al. Solamargine inhibits migration and invasion of human hepatocellular carcinoma cells through down-regulation of matrixmetalloproteinases 2 and 9 expression and activity. Toxicol In Vitro. 2015 Aug;29(5):893-900.3. Ding X, et al. Induction of apoptosis in human hepatoma SMMC-7721 cells

11、by solamargine from Solanum nigrum L. J Ethnopharmacol. 2012 Jan31;139(2):599-604.4. Fu R, et al. Solamargine inhibits gastric cancer progression by regulating the expression of lncNEAT1_2 via the MAPK signaling pathway. Int J Oncol.2019 May;54(5):1545-1554.McePdfHeightCaution: Product has not been fully validated f