Hydrocortisone-Cortisol-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHydrocortisoneCat. No.: HY-N0583CAS No.: 50-23-7Synonyms: Cortisol分式: CHO分量: 362.46作靶点: Glucocorticoid Receptor; Endogenous Metabolite作通路: GPCR/G Protein; Metabolic Enzyme/Protease储存式: 4C, protect from light* In solvent : -80C,

2、6 months; -20C, 1 month (protect fromlight)溶解性数据体外实验 DMSO : 31 mg/mL (85.53 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.7589 mL 13.7946 mL 27.5893 mL5 mM 0.5518 mL 2.7589 mL 5.5179 mL10 mM 0.2759 mL 1.3795 mL 2.7589 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并

3、请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.90 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubil

4、ity: 2.5 mg/mL (6.90 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oil1/3 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 2.5 mg/mL (6.90 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Hydrocortisone肾上腺质分泌的类 醇激素或糖质激素。IC50 & Target Human Endogenous Metabolite体外研究 Hydrocortisone (50 nM) shows

5、 a dose-dependent down-regulation of GR transcript in hCMEC/D3 cells.Hydrocortisone supplementation of the serum-reduced cell differentiation medium leads to a significantincrease in TER across the hCMEC/D3 monolayer 1. Hydrocortisone-treated Dendritic cells (DCs) show adecreased expression of MHC I

6、I molecules, the costimulatory molecule CD86, and the DC-specific markerCD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibit production of IFN-but induce an increased release of IL-4 and no change in IL-5 2. Hydrocortisone reduces postischemicoxidative stress, perf

7、usion pressure, and transudate formation. Hydrocortisone inhibits postischemicshedding of syndecan-1, heparan sulfate, and hyaluronan as is release of histamine from resident mast cells3.PROTOCOLCell Assay 1 Cells are plated on top of collagen IV-coated transwell chambers for six-well plates (24 mm

8、diameter,membrane material: polyethylene terephthalate (PET), 0.4 m pores, pore density 1.6106 cm2) at densitiesof 2.5104 cells cm2 per well. When they have reached confluence at day 5, the different experimental setsof cells are transferred to differentiation medium containing reduced amounts of FC

9、S and treated with TNFor hydrocortisone as indicated.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cancer Manag Res. 2019 Jul. Cancer Manag Res. 2019 Jun 21;11:5557-5572.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1.

10、 F?rster C, et al. Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol. 2008 Apr 1;586(7):1937-49.2. Bellinghausen I, et al. Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: dif

11、ferences from hydrocortisone-treated dendritic cells. J Allergy Clin Immunol. 2001 Aug;108(2):242-9.3. Chappell D, et al. Hydrocortisone preserves the vascular barrier by protecting the endothelial glycocalyx. Anesthesiology. 2007Nov;107(5):776-84.2/3 Master of Small Molecules 您边的抑制剂师www.MedChemEMcePdfHeightCaution: Product has not