分析化学1光盘2

1、High performance liquid chromatography (HPLC) hase the mostversatile and wide application of elution chromatography. The technique isused to separate and determine species in a variety ofanic, inanic, andbiological materials. HPLC method is on the basis of liquid chromatography(LC), in which liquid

2、is a mobile phase. There are four separation principles in classic liquid chromatography: adsorption, distribution, ion exchange andexclu.In classic liquid chromatography, asroduced by Tswett in 1906, glasscolumns had an inner diameter betn 1 and 5 cm and a length betn 50and 500 cm. In order to guar

3、antee the practical flow speeds of mobile phase(up to 1 mLmin-1), particle sizes betn 150 and 200 mm were used.However the separations often took a very long time.The practical transformation succeeded at the end of the 1960s when particles with diameters up to 3 or 10m, as well as a new generation

4、ofinstrument modules wereas ever for preparative purroduced. Classical liquid chromatography is usedes or forroductory experimentso liquidchromatography. This method has been of the characteristic of high prere,fast speed, high performance and high sensitivity.he following section wewill consider in

5、 particular the most importanements of HPLC.第 18 章 高效液相色谱法high performance liquid chromatography ; HPLC 高效液相色谱法liquid-liquid chromatography ; LLC 液液色谱法 liquid-solid chromatography ; LSC 液固色谱法 partition chromatography 分配色谱法adsorption chromatography 吸附色谱法ion exchange chromatography ; IEC 离子交换色谱法;molec

6、ular excluchromatography ; MEC 分子排阻色谱法affinity chromatography ; AC 亲和色谱法 chiral chromatography ; CC 手性色谱法 micellar chromatography ; MC 胶束色谱法electrochromatography ; EC 电色谱法1biochromatography ; BC 生物色谱法ion suppreschromatography ; ISC 离子抑制色谱法paired ion chromatography ; PIC 或 ion pair chromatography ; I

7、PC色谱法chemically bonded-phase chromatography 化学键合相色谱法bonded phase; BP 键合相bonded phase chromatography ; BPC 键合相色谱法octadecylsilane; ODS 或 C18 十八烷基硅烷键合相离子对end-cap封尾ion exchanger 离子交换剂chiral sionary phase；CSP 手性固定相ligand 配基spacer arm 间隔臂high performance affinity chromatography； HPAC 高效亲和色谱polarity parame

8、ter of solvent 溶剂极性参数NP-bonded phase chromatography 正相键合相色谱法 RP-bonded phase chromatography 反相键合相色谱法 RP-ion pair chromatography 反相离子对色谱法 counter ion 反离子(对离子)ion chromatography 离子色谱法chiral sionary phase ; CSP 手性固定相chiral mobile phase additive ; CMPA 手性相添加剂eddy diffu涡流扩散longitudinal diffu纵向扩散sionary p

9、hase mass transfer固定相传质阻抗相传质阻抗mobile phase mass transfersic mobile phase mass transfer静态相传质阻抗ion pair reagent 离子对试剂 chromatograph 色谱仪 pump 泵isocratic 等度gradient 梯度autosler 自动进样装置sling loop 贮样管2load 装样 injection 进样 column 色谱柱narrow bore column 窄径柱detector 检测器 sensitivity 灵敏度 noise 噪音linear range 线性范围repeatability 重复性ultraviolet detector ; UVD 紫外检测器Lambert-Beers law定律photodiode array detector ; PDAD 光电二极管阵列