CAY10602 - Sirtuin Activator - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECAY10602Cat. No.: HY-104073CAS No.: 374922-43-7分式: CHFNOS分量: 418.44作靶点: Sirtuin作通路: Cell Cycle/DNA Damage; Epigenetics储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (238.98 mM; N

2、eed ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.3898 mL 11.9491 mL 23.8983 mL5 mM 0.4780 mL 2.3898 mL 4.7797 mL10 mM 0.2390 mL 1.1949 mL 2.3898 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当

3、天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.97 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.97 mM); Precipitated solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师ww

4、w.MedChemE物活性 CAY10602个 SIRT1 激活剂。IC50 & Target SIRT1体外研究 Compounds (including CAY10602/compound 1) with SIRT1-activating properties have a significant effect onfat mobilization in differentiated adipocytes, and these compounds (including CAY10602) have antiobesityand/or antidiabetic properties. Com

5、pounds (including CAY10602) with SIRT1 activating potential exert strongsuppression of TNF- release at concentrations between 20 and 60 M 1.PROTOCOLCell Assay 1 3T3L1 mouse fibroblasts and THP-1 leukemia cells are used and cultured following the suppliersrecommendations.THP-1 cells are treated first

6、 with representative compounds (includingCAY10602/compound 1) followed by addition of lipopolysaccharide(LPS). THP-1 cells are plated at a densityof 1.2106 cells/mL in 0.4 mL (0.5106 cells/treatment) in 24-well culture plates. The cells are preincubatedwith representative test compounds (including C

7、AY10602) at 2 concentrations (20 or 60 M) for 1 h undergeneral tissue culture conditions 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Death Dis. 2019 Apr 15;10(5):331. Toxicol Appl Pharmacol. 2019 May 11.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Nayagam VM, et al. SIRT1 modulating compounds from high-throughput screening as anti-inflammatory and insulin-sensitizing agents. JBiomol Screen. 2006 Dec;11(8):959-67.McePdfHeightCaution: Product has not been fully validated for medical applications.For