Tectorigenin - Flavonoid - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETectorigeninCat. No.: HY-N0792CAS No.: 548-77-6分式: CHO分量: 300.26作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 150 mg/mL (499.57 mM; Need ultrasonic and warming)Mass

2、Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.3304 mL 16.6522 mL 33.3045 mL5 mM 0.6661 mL 3.3304 mL 6.6609 mL10 mM 0.3330 mL 1.6652 mL 3.3304 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Tectorigenin种植物异 酮，最初 从葛根汤燥的花中分离出来的。体外研究Tectorigenin is a plant isoflavonoid originally

3、 isolated from the dried flower of Pueraria thomsonii Benth.Palmitic acid (PA)-stimulated ROS production is abolished by treatment with Tectorigenin for HUVECs in adose-dependent manner (0.1, 1, 10 M). Treatment with Tectorigenin attenuates enhanced IKKphosphorylation and effectively blocks NF-B act

4、ivation by inhibition of p65 phosphorylation at concentrationsranging from 0.1 to 10 M. Tectorigenin treatment also effectively inhibits PA-augmented TNF- and IL-61/2 Master of Small Molecules 您边的抑制剂师www.MedChemEproduction in a concentration dependent manner 1. The number of viable HepG2 cells treat

5、ed byTectorigenin decreases in a concentration- and time-dependent manner. When HepG2 cells are treated withTectorigenin at 5, 10 and 20 mg/L for 24 h, the viability rate is 91%, 79% and 62%, respectively 2.PROTOCOLKinase Assay 1 HUVECs grown to confluence in 24-well plates are pretreated with Tecto

6、rigenin (0.1, 1, 10 M), salicylate (5mM) or GSH (1 mM) for 30 min, then stimulated with Palmitic acid (PA) (100 M) for further 12 h in serum-free medium, and the medium is then collected on ice. The levels of TNF- and IL-6 in the supernatant areassayed with commercial ELISA Kits 1.MCE has not indepe

7、ndently confirmed the accuracy of these methods. They are for reference only.Cell Assay 2 Cell viability is assessed by MTT method. Briefly, cells are seeded in 96-well plate at a density of 1104cells/well. After 24 h incubation, Tectorigenin at different concentrations is added to the cells while o

8、nlyDMSO (solvent) is added as a negative control. After growing for 12, 24 and 48 h, cells are incubated withMTT (0.5 mg/mL) for 4 h at 37C. During this incubation period, water-insoluble formazan crystals areformed, which are dissolved by the addition of 100 L/well DMSO. The optical densities at 57

9、0 nm aremeasured using an enzyme-linked immunosorbent assay plate reader. Wells containing culture medium andMTT but no cells act as blanks 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Wang Q, et al. Tectorigenin Attenuates Palmitate-I

10、nduced Endothelial Insulin Resistance via Targeting ROS-Associated Inflammationand IRS-1 Pathway. PLoS One. 2013 Jun 19;8(6):e66417.2. Jiang CP, et al. Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells. World J Gastroenterol. 2012 Apr21;18(15):1753-64.McePdfHeightCaution: Product has not been fully valid