Hygromycin-B-Hygrovetine-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEHygromycin BCat. No.: HY-B0490CAS No.: 31282-04-9Synonyms: Hygrovetine分式: CHNO分量: 527.52作靶点: Bacterial; Fungal作通路: Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 H2O : 30 mg/mL (56.

2、87 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.8957 mL 9.4783 mL 18.9566 mL5 mM 0.3791 mL 1.8957 mL 3.7913 mL10 mM 0.1896 mL 0.9478 mL 1.8957 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Hygromycin B抑制原核和真核细胞的氨 糖苷类抗素。IC50 &

3、Target Target：Antibacterial; Antifungal 1体外研究Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEby Streptomyces hygroscopicus 1. Hygromycin B at 0.38 mM concentration completely halts yeast cellg

4、rowth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptidesynthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by lowconcentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast poly

5、somes bypreventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin Bmight result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site 2.体内研究 Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causi

6、ng significantmisreading in vivo 3. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confersresistance to hygromycin B 4.PROTOCOLAnimal Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR areAdministration 4 treated with a sing

7、le dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% foreach consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline.Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10co

8、nsecutive days 4.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Metab. 2018 Oct.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygr

9、oscopicus-the producing organism. J GenMicrobiol. 1985 Jun;131(6):1289-98.2. Gonzlez A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta.1978 Dec 21;521(2):459-69.3. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003May;47(5):1496-502.McePdfHeightCaution: Product has not been fully validated for m